Metabarcoding; Distribution; Phylogeography; Protist; Diversity gradient; Variable marker
Macumber Andrew L., Blandenier Quentin, Todorov Milcho, Duckert Clément, Lara Enrique, Lahr Daniel J.G., Mitchell Edward A.D., Roe Helen M. (2020), Phylogenetic divergence within the Arcellinida (Amoebozoa) is congruent with test size and metabolism type, in European Journal of Protistology
, 72, 125645-125645.
Metz Sebastian, Singer David, Domaizon Isabelle, Unrein Fernando, Lara Enrique (2019), Global distribution of Trebouxiophyceae diversity explored by high‐throughput sequencing and phylogenetic approaches, in Environmental Microbiology
, 21(10), 3885-3895.
Singer David, Mitchell Edward A. D., Payne Richard J., Blandenier Quentin, Duckert Clément, Fernández Leonardo D., Fournier Bertrand, Hernández Cristián E., Granath Gustaf, Rydin Håkan, Bragazza Luca, Koronatova Natalia G., Goia Irina, Harris Lorna I., Kajukało Katarzyna, Kosakyan Anush, Lamentowicz Mariusz, Kosykh Natalia P., Vellak Kai, Lara Enrique (2019), Dispersal limitations and historical factors determine the biogeography of specialized terrestrial protists, in Molecular Ecology
, 28(12), 3089-3100.
Duckert Clément, Blandenier Quentin, Kupferschmid Fanny A.L., Kosakyan Anush, Mitchell Edward A.D., Lara Enrique, Singer David (2018), En garde! Redefinition of Nebela militaris (Arcellinida, Hyalospheniidae) and erection of Alabasta gen. nov., in European Journal of Protistology
, 66, 156-165.
Singer David, Kosakyan Anush, Seppey Christophe V. W., Pillonel Amandine, Fernández Leonardo D., Fontaneto Diego, Mitchell Edward A. D., Lara Enrique (2018), Environmental filtering and phylogenetic clustering correlate with the distribution patterns of cryptic protist species, in Ecology
, 99(4), 904-914.
Geisen Stefan, Mitchell Edward A D, Adl Sina, Bonkowski Michael, Dunthorn Micah, Ekelund Flemming, Fernández Leonardo D, Jousset Alexandre, Krashevska Valentyna, Singer David, Spiegel Frederick W, Walochnik Julia, Lara Enrique (2018), Soil protists: a fertile frontier in soil biology research, in FEMS Microbiology Reviews
, 42(3), 293-323.
Pérez-Juárez Horacio, Serrano-Vázquez Angélica, Kosakyan Anush, Mitchell Edward A.D., Rivera Aguilar Víctor M., Lahr Daniel J.G., Hernández Moreno Mayra M., Cuellar Humberto Macías, Eguiarte Luis E., Lara Enrique (2017), Quadrulella texcalense sp. nov. from a Mexican desert: An unexpected new environment for hyalospheniid testate amoebae, in European Journal of Protistology
, 61, 253-264.
Geisen Stefan, Mitchell Edward A.D., Wilkinson David M., Adl Sina, Bonkowski Michael, Brown Matthew W., Fiore-Donno Anna Maria, Heger Thierry J., Jassey Vincent E.J., Krashevska Valentyna, Lahr Daniel J.G., Marcisz Katarzyna, Mulot Matthieu, Payne Richard, Singer David, Anderson O. Roger, Charman Dan J., Ekelund Flemming, Griffiths Bryan S., Rønn Regin, Smirnov Alexey, Bass David, Belbahri Lassaâd, Berney Cédric, et al. (2017), Soil protistology rebooted: 30 fundamental questions to start with, in Soil Biology and Biochemistry
, 111, 94-103.
Recent environmental barcoding studies are showing that microbial eukaryotes may represent the largest part of the world’s diversity. We are now aware of the existence of previously unsuspected deep branching and ultradiverse protist phyla, which are regularly inflating our estimations on diversity. However, knowledge of diversity at fine taxonomical level (i.e. species level) is still very uneven among groups. This is a major burden for our understanding of their macroecology and biogeography, as all theories have been developed on macroscopic multicellular organisms (mainly plants and animals) and are based on data set with resolution at the species level. As a consequence, we are unable to understand the patterns that govern protist distribution from the landscape to continental scales. In this project, we propose to study the diversity patterns of three clades of protists from a group for which we have a long standing working experience and which is an ideal model for microbial macroecology and biogeography: the hyalospheniid testate amoebae (Amoebozoa; Arcellinida; Hyalospheniidae). We focus specifically at the species level using molecular methods we developed to target specifically this taxonomic resolution level.Our aim in this project is to test how classical macroecological paradigms apply to the distribution of hyalospheniid testate amoebae from the local to the global scale. We will investigate the molecular species-level diversity of three groups that differ deeply in their lifestyles: (A) the Alocodera-Padaungiella group, which has broad ecological tolerance from wet oligotrophic peatlands to forest litter (B) the Nebela tincta complex, which is mainly restricted to peatlands, and (C) the Hyalosphenia papilio species complex, which is mixotrophic and is restricted to oligotrophic habitats in Sphagnum peatlands. For these three groups, we will (I) evaluate the influence of short-term dynamics on the spatial variation of diversity using different classical ecological hypotheses (Rapoport effect, species-energy, water-energy balance and mid-domain effect) and (II) test historical hypotheses to account for the possible influence of long-term dynamic in shaping patterns of geographic distribution. These hypotheses will be tested on the relevant groups in an altitudinal gradient (at the Swiss scale), a circumboreal extensive sampling and a broad sampling in the Southern Neotropics.