Hippocampus; Neurogenesis; Subventricular zone; Brain; Neural stem cells
Vogt Miriam A., Ehsaei Zahra, Knuckles Philip, Higginbottom Adrian, Helmbrecht Michaela S., Kunath Tilo, Eggan Kevin, Williams Luis A., Shaw Pamela J., Wurst Wolfgang, Floss Thomas, Huber Andrea B., Taylor Verdon (2018), TDP-43 induces p53-mediated cell death of cortical progenitors and immature neurons, in Scientific Reports
, 8(1), 8097-8097.
Engler Anna, Zhang Runrui, Taylor Verdon (2018), Molecular Mechanisms of Notch Signaling
, Springer International Publishing, Cham.
Zhang Runrui, Engler Anna, Taylor Verdon (2018), Notch: an interactive player in neurogenesis and disease, in Cell and Tissue Research
, 371(1), 73-89.
Engler Anna, Rolando Chiara, Giachino Claudio, Saotome Ichiko, Erni Andrea, Brien Callum, Zhang Runrui, Zimber-Strobl Ursula, Radtke Freddy, Artavanis-Tsakonas Spyros, Louvi Angeliki, Taylor Verdon (2018), Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone, in Cell Reports
, 22(4), 992-1002.
Fedele Stefania, Collo Ginetta, Behr Katharina, Bischofberger Josef, Müller Stephan, Kunath Tilo, Christensen Klaus, Gündner Anna Lisa, Graf Martin, Jagasia Ravi, Taylor Verdon (2017), Expansion of human midbrain floor plate progenitors from induced pluripotent stem cells increases dopaminergic neuron differentiation potential, in Scientific Reports
, 7(1), 6036-6036.
Rolando Chiara, Taylor Verdon (2017), Non-canonical post-transcriptional RNA regulation of neural stem cell potential, in Brain Plasticity
, 3(1), 111-116.
Pfurr Sabrina, Chu Yu-Hsuan, Bohrer Christian, Greulich Franziska, Beattie Robert, Mammadzada Könül, Hils Miriam, Arnold Sebastian J., Taylor Verdon, Schachtrup Kristina, Uhlenhaut N. Henriette, Schachtrup Christian (2017), The E2A splice variant E47 regulates the differentiation of projection neurons via p57(KIP2) during cortical development, in Development
, 144(21), 3917-3931.
Boareto Marcelo, Iber Dagmar, Taylor Verdon (2017), Differential interactions between Notch and ID factors control neurogenesis by modulating Hes factor autoregulation, in Development
, 144(19), 3465-3474.
Mansouri Maysam, Ehsaei Zahra, Taylor Verdon, Berger Philipp (2017), Baculovirus-based genome editing in primary cells, in Plasmid
, 90, 5-9.
Mansouri Maysam, Bellon-Echeverria Itxaso, Rizk Aurélien, Ehsaei Zahra, Cianciolo Cosentino Chiara, Silva Catarina S., Xie Ye, Boyce Frederick M., Davis M. Wayne, Neuhauss Stephan C. F., Taylor Verdon, Ballmer-Hofer Kurt, Berger Imre, Berger Philipp (2016), Highly efficient baculovirus-mediated multigene delivery in primary cells, in Nature Communications
, 7(1), 11529-11529.
Rolando Chiara, Erni Andrea, Grison Alice, Beattie Robert, Engler Anna, Gokhale Paul J., Milo Marta, Wegleiter Thomas, Jessberger Sebastian, Taylor Verdon (2016), Multipotency of Adult Hippocampal NSCs In Vivo Is Restricted by Drosha/NFIB, in Cell Stem Cell
, 19(5), 653-662.
Giachino Claudio, Boulay Jean-Louis, Ivanek Robert, Alvarado Alvaro, Tostado Cristobal, Lugert Sebastian, Tchorz Jan, Coban Mustafa, Mariani Luigi, Bettler Bernhard, Lathia Justin, Frank Stephan, Pfister Stefan, Kool Marcel, Taylor Verdon (2015), A Tumor Suppressor Function for Notch Signaling in Forebrain Tumor Subtypes, in Cancer Cell
, 28(6), 730-742.
Lojewski Xenia, Srimasorn Sumitra, Rauh Juliane, Francke Silvan, Wobus Manja, Taylor Verdon, Araúzo-Bravo Marcos J., Hallmeyer-Elgner Susanne, Kirsch Matthias, Schwarz Sigrid, Schwarz Johannes, Storch Alexander, Hermann Andreas (2015), Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation PotentialAdult Human Brain-Derived Pericytes, in STEM CELLS Translational Medicine
, 4(10), 1223-1233.
Wang Yidong, Wu Bingruo, Farrar Emily, Lui Wendy, Lu Pengfei, Zhang Donghong, Alfieri Christina M., Mao Kai, Chu Ming, Yang Di, Xu Di, Rauchman Michael, Taylor Verdon, Conway Simon J., Yutzey Katherine E., Butcher Jonathan T., Zhou Bin (2015), Notch-Tnf signalling is required for development and homeostasis of arterial valves, in European Heart Journal
Azim Kasum, Hurtado-Chong Anahí, Fischer Bruno, Kumar Nitin, Zweifel Stefan, Taylor Verdon, Raineteau Olivier (2015), Transcriptional Hallmarks of Heterogeneous Neural Stem Cell Niches of the Subventricular ZoneRegional Transcriptome of the Subventricular Zone, in STEM CELLS
, 33(7), 2232-2242.
Moretti Francesca, Rolando Chiara, Winker Moritz, Ivanek Robert, Rodriguez Javier, Von Kriegsheim Alex, Taylor Verdon, Bustin Michael, Pertz Olivier (2015), Growth Cone Localization of the mRNA Encoding the Chromatin Regulator HMGN5 Modulates Neurite Outgrowth, in Molecular and Cellular Biology
, 35(11), 2035-2050.
Nato G., Caramello A., Trova S., Avataneo V., Rolando C., Taylor V., Buffo A., Peretto P., Luzzati F. (2015), Striatal astrocytes produce neuroblasts in an excitotoxic model of Huntington's disease, in Development
, 142(5), 840-845.
Boda Enrica, Di Maria Silvia, Rosa Patrizia, Taylor Verdon, Abbracchio Maria P., Buffo Annalisa (2015), Early phenotypic asymmetry of sister oligodendrocyte progenitor cells after mitosis and its modulation by aging and extrinsic factorsSister OPC Asymmetry after Mitosis, in Glia
, 63(2), 271-286.
The regulation of neural stem cell (NSC) maintenance and differentiation is crucial for formation of the central nervous system (CNS). A detailed understanding of NSC biology has important implications for comprehending congenital brain malformations, age-related disorders, neurological diseases and regeneration. Established genetic tools, in vivo and in vitro experimental approaches for manipulation and lineage tracing, make neural progenitors an attractive experimental paradigm. The patterned and structured formation of the mammalian brain and positional fate restriction assists in the analysis of phenotypes and gene functions. Further, congenital brain malformations can often be traced to aberrant proliferation, differentiation or specification of neural progenitors. The production of new neurons also plays important roles in the adult mammalian brain. In rodents, neurons of the olfactory system are continually regenerated from the subventricular zone (SVZ), rebuilding complex multi-neuron circuits. New neurons in the hippocampal dentate gyrus (DG) are important for specific forms of memory and learning in mice. Although the role of neurogenesis in the human brain is debated, links to pathologies including epilepsy, depression and brain tumors underline the importance of understanding the mechanism controlling NSCs. The potential of NSC for regeneration and rejuvenation also emphasize a need to fill the gaps in our basic knowledge of NSC biology.My lab has focused on the role of Notch signaling in NSC maintenance1-4. By generating and combining transgenic mice with analysis of homeostasis, physical activity, degeneration, regeneration aging and in vitro approaches, we determined that NSCs are a heterogeneous cell population even within the same niche1,2,4-6. Recently, we found that the adult SVZ and DG contain active and dormant NSCs1,5-7. Active NSCs express brain lipid binding protein (BLBP), drive neurogenesis, and there loss is one potential cause of the age-dependent decline in neurogenesis. BLBP+ active NSCs have not been studied in detail, either during homeostasis, aging nor regeneration. We have generated tools including transgenic mice to study these active and dormant NSCs in greater detail. We will use these tools to continue to deepen our knowledge of NSCs in the brain. We will lineage trace different NSC populations in vivo, addressing dormant, quiescent and active NSCs and elucidating their roles in homeostasis and regeneration. We will study the effects that pathophysiological stimuli have on these NSC populations. Recently we found that active NSCs depend upon Notch1 but dormant NSCs do not. Dormant NSCs express Notch2 and Notch3, hence, we will assess the functions of Notch1, Notch2 and Notch3 in the different NSCs populations of the brain. The control of NSC activity and dormancy has clinical relevance for regeneration but also for rejuvenation to combat age-related disorders. We have generated transcription profiles of embryonic and adult Hes5::GFP+ NSCs and of Notch1-regulated genes in NSCs. Many components of the microRNA (miRNA) biogenesis pathway were present in these profiles. Therefore, we targeted the root of miRNA biogenesis, conditionally ablating key components of the miRNA microprocessor (MP) in forebrain NSCs. We established that the MP and Drosha, unlike Dicer, play a central role in regulating NSCs during development. An important function of the MP in NSCs is a novel miRNA-independent mechanism to directly target stem-loop hairpin structures in the Ngn2 mRNA. We will extend our previous findings of MP function in neurogenesis, identify novel targets of the MP and extend the analysis of MP function to active and dormant NSCs during aging and regeneration.