EEG; sleep; TMS; optogenetic; neuroplasticity; stroke recovery; slow wave activity; stroke; cortex; bistability
Colombo Michele Angelo, Napolitani Martino, Boly Melanie, Gosseries Olivia, Casarotto Silvia, Rosanova Mario, Brichant Jean-Francois, Boveroux Pierre, Rex Steffen, Laureys Steven, Massimini Marcello, Chieregato Arturo, Sarasso Simone (2019), The spectral exponent of the resting EEG indexes the presence of consciousness during unresponsiveness induced by propofol, xenon, and ketamine, in NeuroImage
, 189, 631-644.
Mensen Armand, Pigorini Andrea, Facchin Laura, Schöne Cornelia, D'Ambrosio Sasha, Jendoubi Jasmine, Jaramillo Valeria, Chiffi Kathrin, Eberhard-Moscicka Aleksandra K., Sarasso Simone, Adamantidis Antoine, Müri René M., Huber Reto, Massimini Marcello, Bassetti Claudio (2019), Sleep as a model to understand neuroplasticity and recovery after stroke: Observational, perturbational and interventional approaches, in Journal of Neuroscience Methods
, 313, 37-43.
CattaniAnna (2018), Abstracts - Cortical perturbations reveal local sleep-like down states in cortical perilesional area, in Journal of Sleep Research
, 27, e12751-e12751.
Bola Michał, Barrett Adam B., Pigorini Andrea, Nobili Lino, Seth Anil K., Marchewka Artur (2018), Loss of consciousness is related to hyper-correlated gamma-band activity in anesthetized macaques and sleeping humans, in NeuroImage
, 167, 130-142.
Rosanova Mario, Sarasso Simone, Pigorini Andrea, Massimini Marcello (2018), Sleep-like bistability, loss of causality and complexity in the brain of Unresponsive Wakefulness Syndrome patients, in bioRxiv
Fecchio Matteo, Pigorini Andrea, Comanducci Angela, Sarasso Simone, Casarotto Silvia, Premoli Isabella, Derchi Chiara-Camilla, Mazza Alice, Russo Simone, Resta Federico, Ferrarelli Fabio, Mariotti Maurizio, Ziemann Ulf, Massimini Marcello, Rosanova Mario (2017), The spectral features of EEG responses to transcranial magnetic stimulation of the primary motor cortex depend on the amplitude of the motor evoked potentials., in PloS one
, 12(9), 0184910-0184910.
Pincherle Alessandro, Pace Marta, Sarasso Simone, Facchin Laura, Dreier Jens P., Bassetti Claudio L. (2017), Sleep, Preconditioning and Stroke, in Stroke
, 48(12), 3400-3407.
Schartner Michael M., Pigorini Andrea, Gibbs Steve A., Arnulfo Gabriele, Sarasso Simone, Barnett Lionel, Nobili Lino, Massimini Marcello, Seth Anil K., Barrett Adam B. (2017), Global and local complexity of intracranial EEG decreases during NREM sleep, in Neuroscience of Consciousness
Major advances were made in stroke prevention and acute management. Conversely, recovery from stroke can be promoted only by neurorehabilitation. Animal and human data have shown that this occurs thank to neuroplasticity processes involving ipsi- and contralesional areas. Since sleep promotes learning and memory, and more generally neuroplasticity, we postulate that sleep-related mechanisms may modulate stroke recovery. Specifically, we hypothesize that perilesional slow waves -which reflect neuronal bistability (an alternation between depolarized ON and hyperpolarized OFF states)- may have a favorable function in the initial phase post-stroke promoting homeostatic adjustments of cortical excitability, while beeing detrimental in the chronic phase because disrupting intracortical information processing.In order to test these hypotheses, we designed a three-steps research strategy:1) observational approach: ipsi- and contralesional sleep-wake high density EEG changes will be assessed in rodents and human with hemispheric stroke from the acute to the chronic stage and correlated with functional outcome;2) perturbational approach: the reactivity and connectivity of the perilesional cortex -as measures of cortical bistability- will be assessed by transcranial magnetic stimulation (TMS) during functional recovery after stroke in humans; 3) interventional approach: manipulations of cortical bistability will be performed in mice (with optogenetics) and humans (with TMS) after stroke and assessed in terms of their effects on functional outcome.The multimodal and translational approach chosen for this proposal has never been used previously in this context. The methodological feasibility of the animal and clinical experiments and the ability to recruite stroke patients for clinical-neurophysiological projects were repeatedly proven in the past by the four research teams. The demonstration that sleep related processes may play a role in neuroplasticity and functional outcome after stroke and the understanding of the network and cellular mechanisms involved are expected to have major neuroscientific and clinical implications.