membrane protein; cryo-electro tomography; structure; proteoliposome; 2D crystal; transmission electron microscopy
Hirschi S., Fischer N., Kalbermatter D., Laskowski P. R., Ucurum Z., Müller D. J., Fotiadis D. (2019), Design and assembly of a chemically switchable and fluorescently traceable light-driven proton pump system for bionanotechnological applications, in Scientific Reports
, 9(1), 1046-1046.
Jeckelmann Jean-Marc, Fotiadis Dimitrios (2019), Volta Phase Plate Cryo-EM Structure of the Human Heterodimeric Amino Acid Transporter 4F2hc-LAT2, in International Journal of Molecular Sciences
, 20(4), 931-931.
Thoma Johannes, Manioglu Selen, Kalbermatter David, Bosshart Patrick D., Fotiadis Dimitrios, Müller Daniel J. (2018), Protein-enriched outer membrane vesicles as a native platform for outer membrane protein studies, in Communications Biology
, 1(1), 23-23.
Ritzmann Noah, Thoma Johannes, Hirschi Stephan, Kalbermatter David, Fotiadis Dimitrios, Müller Daniel J. (2017), Fusion Domains Guide the Oriented Insertion of Light-Driven Proton Pumps into Liposomes, in Biophysical Journal
, 113(6), 1181-1186.
Low electron-dose imaging of biological samples by cryo-transmission electron microscopy (cryo-TEM) has experienced a major revolution in the last year. Cameras based on a groundbreaking new technology directly detect electrons. This is in contrast to conventional CCD cameras, which convert electrons to light with a scintillator before read-out. The new cameras, termed direct electron detector devices (DDDs), have unprecedented high sensitivity and signal-to-noise ratio. Due to the much improved sensitivity of DDDs, recording of ’movies’ and subsequent correction of electron beam-induced drift are possible. The drift correction together with the high signal-to-noise ratio of DDDs dramatically improves contrast and resolution of electron micrographs making possible the determination of macromolecule structures at near-atomic resolution by cryo-TEM and single particle 3D reconstruction. An additional important improvement of contrast will be achieved by the requested first commercial phase plate that will be made available to the cryo-TEM community by the FEI company at the end of 2014. The phase plate technology is particularly attractive for single particle analysis of relatively small macromolecules and for cryo-electron tomography.The applicants are established and internationally recognized experts in the fields of transmission electron microscopy (TEM) and cryo-electron tomography of biological samples. For them and the University of Bern, the here requested DDD camera and phase plate are essential in order to stay at the forefront in the field of TEM and competitive at the international level. Nine research groups from the Medical, Life and Natural Sciences, and Veterinary Medical Faculties of the University of Bern will directly benefit from these new technologies. The requested equipment will be mounted on the FEI Tecnai F20 field emission gun electron microscope. This high-end electron microscope is located at the Institute of Anatomy, which is the leading house of the interfaculty Microscopy Imaging Centre (MIC) of the University of Bern. Through the MIC, users from the University of Bern as well as external users from other public and private research institutions will have the possibility to access and benefit from this state-of-the art equipment.