Project

Back to overview

Assessing metal bioavailability through transcriptome response in aquatic primary producers (continuation)

English title Assessing metal bioavailability through transcriptome response in aquatic primary producers (continuation)
Applicant Slaveykova-Startcheva Vera
Number 157173
Funding scheme Project funding (Div. I-III)
Research institution Institut F.-A. Forel Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Other disciplines of Environmental Sciences
Start/End 01.03.2015 - 31.07.2016
Approved amount 75'797.00
Show all

Keywords (5)

macrophyte; bioavailability; microalgae; DOM; genomics

Lay Summary (French)

Lead
Évaluation de la biodisponibilité des métaux par l’analyse du transcriptome chez les producteurs primaires aquatiques
Lay summary

Ce projet a pour objectif de poursuivre l’évaluation du lien entre la biodisponibilité des métaux toxiques et l’expression des gènes chez deux producteurs primaires aquatiques représentatifs, dans des conditions environnementales pertinentes, notamment en présence de matière organique dissoute (MOD). La bioaccumulation ainsi que les effets induits du mercure inorganique et du méthylmercure seront comparés chez le macrophyte submergé Elodea nuttallii et la microalgue Chlamydomonas reinhardtii. En effet, leurs différences d’habitat et de morphologie créent des divergences notables en termes de durée d’exposition aux métaux traces et de la quantité bioaccumulée de ces derniers. La biodisponibilité de deux spéciations du mercure (mercure inorganique HgCl2 et mercure organique CH3HgCl) et celle du cuivre en présence de MOD, affectant potentiellement celle-ci, ainsi que les effets sur d’autres paramètres tels que la croissance, la photosynthèse et l’expression des gènes seront comparées pour chaque organisme. La biodisponibilité sera déterminée par la mesure des concentrations de métaux adsorbés et internalisés à différents temps d'exposition. La comparaison des résultats permettra de déterminer le degré de similarité des réponses du transcriptome des producteurs primaires face aux métaux biodisponibles. Les approches proposées contribueront à l’amélioration de notre compréhension de la biodisponibilité des métaux chez les producteurs primaires aquatiques. En particulier, la spéciation et l’assimilation du mercure seront étudiées et comparées au niveau d’expression d’un groupe de gènes sélectionnés chez le macrophyte et la microalgue. Ces connaissances fondamentales pourraient aider à développer de nouveaux outils dans le domaine de l’écotoxicologie. Effectivement, la surveillance de la biodisponibilité des métaux toxiques est cruciale pour une évaluation du risque environnemental efficace.


Direct link to Lay Summary Last update: 19.12.2014

Responsible applicant and co-applicants

Employees

Publications

Publication
Towards early-warning gene signature of Chlamydomonas reinhardtii exposed to Hg-containing complex media
Beauvais-Flück Rébecca, Slaveykova Vera I., Ulf Skyllberg, Cosio Claudia (2019), Towards early-warning gene signature of Chlamydomonas reinhardtii exposed to Hg-containing complex media, in Aquatic Toxicology, 214, 105259-105259.
Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii
Beauvais-Flück Rebecca, Slaveykova Vera I., Cosio Claudia (2019), Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii, in Environmental Pollution, 250, 331-337.
Molecular Effects, Speciation, and Competition of Inorganic and Methyl Mercury in the Aquatic Plant Elodea nuttallii
Beauvais-Flück Rébecca, Slaveykova Vera I., Skyllberg Ulf, Cosio Claudia (2018), Molecular Effects, Speciation, and Competition of Inorganic and Methyl Mercury in the Aquatic Plant Elodea nuttallii, in Environmental Science & Technology, 52(15), 8876-8884.
Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii
Beauvais-Flück Rebecca, Slaveykova Vera, Cosio Claudia (2017), Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii, in Sci Rep., 7(8034), 1-11.
Role of cellular compartmentalization in the trophic transfer of mercury species in a freshwater plant-crustacean food chain
Beauvais-Flück rebecca, Chaumot Arnaud, Gimbert Frédéric, Quéau Hervé, Geffard Olivier, Slaveykova Vera I, Cosio Claudia (2016), Role of cellular compartmentalization in the trophic transfer of mercury species in a freshwater plant-crustacean food chain, in Journal of Hazardous Materials, 320, 401-407.
Transcriptomic and Physiological Responses of the Green Microalga Chlamydomonas reinhardtii during Short-Term Exposure to Subnanomolar Methylmercury Concentrations
Beauvais-Flück Rebecca, Slaveykova Vera I, Cosio Claudia (2016), Transcriptomic and Physiological Responses of the Green Microalga Chlamydomonas reinhardtii during Short-Term Exposure to Subnanomolar Methylmercury Concentrations, in Environmental Science and Technology, 50(13), 7126-7134.

Collaboration

Group / person Country
Types of collaboration
Genomic platform UniGE Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Interfaces against pollution Talk given at a conference Mercury species bioavailability to aquatic primary producers: from the cell to the community level 05.09.2016 Lleida, Spain Slaveykova-Startcheva Vera;
XXXIII congress of the International scoiety of limnology Poster Uptake and effects of mercury compounds to phytoplankton: from molecule to cell 31.07.2016 Torino, Italy Slaveykova-Startcheva Vera;
26th annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Dietary transfer of Hg from Elodea nuttallii to Gammarus fossarum 23.05.2016 Nantes, France Cosio Claudia; Slaveykova-Startcheva Vera;
26th annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Poster Mercury bioavailability and toxicity to green microalga 22.05.2016 Nantes, France Slaveykova-Startcheva Vera; Cosio Claudia;
20. SETAC-GLB Jahrestagung Ökotoxikologie und Umweltchemie – von der Forschung in die Praxis Talk given at a conference Effects of methyl-mercury on aquatic primary producers: a transcriptomic approach 07.09.2015 Zurich, Switzerland Beauvais-Flueck Rebecca;
9th Symposium for European Freshwater Sciences Talk given at a conference Transcriptomic response of the aquatic macrophyte Elodea nuttallii to Hg exposure under enhanced UV radiation 05.07.2015 Geneva, Switzerland Beauvais-Flueck Rebecca; Cosio Claudia;
25th annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Linking transcriptome response with mercury bioaccumulation in the microalga Chlamydomonas reinhardti 03.05.2015 Barcelona, Spain Slaveykova-Startcheva Vera; Cosio Claudia; Beauvais-Flueck Rebecca;


Communication with the public

Communication Title Media Place Year
Media relations: radio, television Le mercure peut-il altérer l’expression des gènes? Radiocite Western Switzerland 2017
Media relations: radio, television Même à faible dose, le mercure altère les gènes, selon une étude de l'UNIGE RTS Western Switzerland 2017

Associated projects

Number Title Start Funding scheme
142228 Mercury threat in industrially impacted surface water bodies in Romania - integrated approach (MERCURO) 01.01.2013 Romanian-Swiss Research Programme (RSRP)
175721 Elucidating the role of PHYToplankton in Aquatic Mercury (Hg) Biotic and Abiotic transformations (PHYTAMBA) 01.03.2018 Project funding (Div. I-III)
138254 Assessing metal bioavailability through transcriptome response in aquatic primary producers 01.03.2012 Project funding (Div. I-III)
134627 Metal Bioavailability to Aquatic Photosynthetic Organisms in Changing Environment (BioChEn) 01.09.2011 Project funding (Div. I-III)
166089 Bioavailability of Mercury Species to Phytoplankton (BioMer) 01.10.2016 Project funding (Div. I-III)

Abstract

This project aims to further assess the relationship between toxic metal bioavailability and gene expression in two representative aquatic primary producers in environmentally realistic conditions, namely in presence of dissolved organic matter (DOM). Mercury and methylmercury bioaccumulation and effects induced in rooted macrophyte Elodea nuttalli and a green microalga Chlamydomonas reinhardtii, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column, will be compared. Indeed, they difference in habitat and morphology creates substantial differences with respect to the exposure length to and amount bioaccumulated of trace metals. Bioavailability of two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) and Cu in presence of DOM putatively affecting bioavailability will be compared as well as other endpoints including growth, photosynthesis and gene expression level in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. Responses will be compared to determine how similar primary producers’ transcriptomics responses to bioavailable metals are. The proposed approaches will contribute to further improve our understanding of bioavailability of metals to aquatic primary producers. In particular speciation and uptake of Hg will be studied and compared to gene expression level of selected set of genes in microalgae and macrophyte. This fundamental knowledge might help to develop new tools for the field of ecotoxicology. Indeed, monitoring bioavailability of toxicants is crucial for efficient environmental risk assessment
-