Beauvais-Flück Rebecca, Slaveykova Vera, Cosio Claudia (2017), Cellular toxicity pathways of inorganic and methyl mercury in the green microalga Chlamydomonas reinhardtii, in Sci Rep.
, 7(8034), 1-11.
Beauvais-Flück rebecca, Chaumot Arnaud, Gimbert Frédéric, Quéau Hervé, Geffard Olivier, Slaveykova Vera I, Cosio Claudia (2016), Role of cellular compartmentalization in the trophic transfer of mercury species in a freshwater plant-crustacean food chain, in Journal of Hazardous Materials
, 320, 401-407.
Beauvais-Flück Rebecca, Slaveykova Vera I, Cosio Claudia (2016), Transcriptomic and Physiological Responses of the Green Microalga Chlamydomonas reinhardtii during Short-Term Exposure to Subnanomolar Methylmercury Concentrations, in Environmental Science and Technology
, 50(13), 7126-7134.
This project aims to further assess the relationship between toxic metal bioavailability and gene expression in two representative aquatic primary producers in environmentally realistic conditions, namely in presence of dissolved organic matter (DOM). Mercury and methylmercury bioaccumulation and effects induced in rooted macrophyte Elodea nuttalli and a green microalga Chlamydomonas reinhardtii, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column, will be compared. Indeed, they difference in habitat and morphology creates substantial differences with respect to the exposure length to and amount bioaccumulated of trace metals. Bioavailability of two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) and Cu in presence of DOM putatively affecting bioavailability will be compared as well as other endpoints including growth, photosynthesis and gene expression level in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. Responses will be compared to determine how similar primary producers’ transcriptomics responses to bioavailable metals are. The proposed approaches will contribute to further improve our understanding of bioavailability of metals to aquatic primary producers. In particular speciation and uptake of Hg will be studied and compared to gene expression level of selected set of genes in microalgae and macrophyte. This fundamental knowledge might help to develop new tools for the field of ecotoxicology. Indeed, monitoring bioavailability of toxicants is crucial for efficient environmental risk assessment