chromatin structure; nuclear organization; DNA repair; genome stability; heterochromatin
Ahringer Julie, Gasser Susan M (2018), Repressive Chromatin in Caenorhabditis elegans: Establishment, Composition, and Function., in Genetics
, 208(2), 491-511.
Hauer Michael H, Gasser Susan M (2017), Chromatin and nucleosome dynamics in DNA damage and repair., in Genes & development
, 31(22), 2204-2221.
Zeller Peter, Gasser Susan M (2017), The Importance of Satellite Sequence Repression for Genome Stability., in Cold Spring Harbor symposia on quantitative biology
Jeggo Penny A, Downs Jessica A, Gasser Susan M (2017), Chromatin modifiers and remodellers in DNA repair and signalling., in Philosophical transactions of the Royal Society of London. Series B, Biological sciences
, 372(1731), 20160279.
Deshpande Ishan, Seeber Andrew, Shimada Kenji, Keusch Jeremy J, Gut Heinz, Gasser Susan M (2017), Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage., in Molecular cell
, 68(2), 431-445.
Poli Jérôme, Gasser Susan M, Papamichos-Chronakis Manolis (2017), The INO80 remodeller in transcription, replication and repair., in Philosophical transactions of the Royal Society of London. Series B, Biological sciences
, 372(1731), 20160290.
Seeber Andrew, Gasser Susan M (2017), Chromatin organization and dynamics in double-strand break repair., in Current opinion in genetics & development
, 43, 9-16.
Hauer Michael H, Seeber Andrew, Singh Vijender, Thierry Raphael, Sack Ragna, Amitai Assaf, Kryzhanovska Mariya, Eglinger Jan, Holcman David, Owen-Hughes Tom, Gasser Susan M (2017), Histone degradation in response to DNA damage enhances chromatin dynamics and recombination rates., in Nature structural & molecular biology
, 24(2), 99-107.
Shimada Kenji, Gasser Susan M (2017), A game of musical chairs: Pro- and anti-resection factors compete for TOPBP1 binding after DNA damage., in The Journal of cell biology
, 216(3), 535-537.
Amitai Assaf, Seeber Andrew, Gasser Susan M., Holcman David (2017), Visualization of Chromatin Decompaction and Break Site Extrusion as Predicted by Statistical Polymer Modeling of Single-Locus Trajectories, in Cell Reports
, 18(5), 1200-1214.
Gonzalez-Sandoval Adriana, Gasser Susan M (2016), Mechanism of chromatin segregation to the nuclear periphery in C. elegans embryos., in Worm
, 5(3), 1190900-1190900.
Gasser Susan M (2016), Selfish DNA and Epigenetic Repression Revisited., in Genetics
, 204(3), 837-839.
Seeber Andrew, Hegnauer Anna Maria, Hustedt Nicole, Deshpande Ishan, Poli Jérôme, Eglinger Jan, Pasero Philippe, Gut Heinz, Shinohara Miki, Hopfner Karl-Peter, Shimada Kenji, Gasser Susan M (2016), RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to hold Sister Chromatids Together, in Molecular cell
, 64(5), 951-966.
Zeller Peter, Padeken Jan, van Schendel Robin, Kalck Veronique, Tijsterman Marcel, Gasser Susan M (2016), Histone H3K9 methylation is dispensable for Caenorhabditis elegans development but suppresses RNA:DNA hybrid-associated repeat instability., in Nature genetics
, 48(11), 1385-1395.
Horigome Chihiro, Gasser Susan M (2016), SUMO wrestles breaks to the nuclear ring's edge., in Cell cycle (Georgetown, Tex.)
, 15(22), 3011-3013.
Gonzalez-Sandoval Adriana, Gasser Susan M. (2016), On TADs and LADs: Spatial Control Over Gene Expression, in TRENDS IN GENETICS
, 32(8), 485-495.
Gasser Susan M. (2016), Nuclear Architecture: Past and Future Tense, in TRENDS IN CELL BIOLOGY
, 26(7), 473-475.
Cabianca Daphne S, Gasser Susan M (2016), Spatial segregation of heterochromatin: Uncovering functionality in a multicellular organism., in Nucleus (Austin, Tex.)
, 7(3), 301-7.
Horigome Chihiro, Bustard Denise E., Marcomini Isabella, Delgoshaie Neda, Tsai-Pflugfelder Monika, Cobb Jennifer A., Gasser Susan M. (2016), PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL, in GENES & DEVELOPMENT
, 30(8), 931-945.
Iwasaki Daichi, Hayashihara Kayoko, Shima Hiroki, Higashide Mika, Terasawa Masahiro, Gasser Susan M., Shinohara Miki (2016), The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA-Dependent Tel1 Activation, in PLOS GENETICS
, 12(3), 1005942.
Harr Jennifer C., Gonzalez-Sandoval Adriana, Gasser Susan M. (2016), Histones and histone modifications in perinuclear chromatin anchoring: from yeast to man, in EMBO REPORTS
, 17(2), 139-155.
Poli Jerome, Gerhold Christian-Benedikt, Tosi Alessandro, Hustedt Nicole, Seeber Andrew, Sack Ragna, Herzog Franz, Pasero Philippe, Shimada Kenji, Hopfner Karl-Peter, Gasser Susan M. (2016), Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress, in GENES & DEVELOPMENT
, 30(3), 337-354.
Our research focuses on two key lines of research: mechanisms that ensure genomic stability during replication and repair of the genome in yeast, and the mechanisms that spatially organize and stabilize chromatin during C. elegans development. We use live microscopy techniques and Chromatin immunoprecipitation to analyse the long-range organization of chromatin in the nucleus, and we dissect the mechanisms and consequences of its perturbation in mutants. Recent work examines how the position and dynamics of chromatin loci affect the repair of double strand breaks or of endogenous damage that arises during replication. Our work on heterochromatin organization in C. elegans has been ground-breaking and has led to identification of a histone methylation event (H3K9me) as a necessary targeting signal for chromatin binding to the nuclear periphery. Juxtaposition to the nuclear envelope is known to affect DNA repair both in yeast and higher eukaryotes. We are pursuing the mechanism of positioning and the impact that either loss of localization and/or H3K9me has on genome stability in worms. In yeast we are examine the molecular crosstalk of checkpoint kinases, nucleosome remodelers, and the subdiffusive mobility of chromatin (and its ultimate subnuclear position) during various repair events. Our goal is to understand how local and long-range chromatin structures influence genome stability.