structural biology; live cell imaging; RNA localization; RNA-protein complex
Voigt Franka, Eglinger Jan, Chao Jeffrey A. (2018), Detection of the First Round of Translation: The TRICK Assay.
, Springer New York, New York, NY.
Voigt Franka, Zhang Hui, Cui Xianying A., Triebold Désirée, Liu Ai Xin, Eglinger Jan, Lee Eliza S., Chao Jeffrey A., Palazzo Alexander F. (2017), Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum, in Cell Reports
, 21(13), 3740-3753.
Horvathova Ivana, Voigt Franka, Kotrys Anna V., Zhan Yinxiu, Artus-Revel Caroline G., Eglinger Jan, Stadler Michael B., Giorgetti Luca, Chao Jeffrey A. (2017), The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells, in Molecular Cell
, 68(3), 615-625.e9.
Halstead J.M., Wilbertz J.H., Wippich F., Lionnet T., Ephrussi A., Chao J.A. (2016), TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals., in Jaffrey Samie (ed.), Elsevier, USA, 123-157.
The spatial and temporal control of gene expression is an evolutionarily conserved mechanism for restricting protein synthesis to discrete sub-cellular locations at the time when the gene product is needed. Neurons have elaborate cellular morphologies with intricate dendritic arbors and long-ranging axons that make thousands of synaptic connections to other neurons which must be individually modified in order to process information. This precise control of gene expression is achieved, in part, by the transport of specific mRNAs from the soma to these remote sites of cell-to-cell contact where they can then be locally translated. We propose to investigate the molecular mechanisms that regulate such finely tuned gene expression by applying a combination of single-molecule live cell imaging techniques with molecular biology, biochemistry and structural biology. Since defects in regulation of local translation in neurons are thought to contribute to a number of neurological and neuromuscular disorders, our studies may provide a molecular foundation for understanding these diseases.