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Phylogeny, total-evidence dating, and the impact of parasitoid lifestyle on the diversification of pimpliforme parasitoid wasps (Hymenoptera, Ichneumonidae)

Applicant Klopfstein Seraina
Number 154791
Funding scheme Ambizione
Research institution Abteilung Synökologie Institut für Ökologie und Evolution Universität Bern
Institution of higher education University of Berne - BE
Main discipline Zoology
Start/End 01.03.2015 - 30.09.2018
Approved amount 600'000.00
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All Disciplines (2)

Discipline
Zoology
Paleontology (biol.)

Keywords (8)

parasitoid wasps; fossils; molecular; dated tree; diversification; morphology; transcriptome; phylogeny

Lay Summary (German)

Lead
Mehr als 4.5% aller beschriebener Arten sind parasitoide Wespen. Diese Insektengruppe ist noch sehr unzureichend erforscht, und wir haben kaum eine Erklärung für ihre enorme Artenvielfalt. Wir erstellen deshalb einen molekularen und morphologischen Stammbaum einer Gruppe der Echten Schlupfwespen, schätzen ihr Alter mit Hilfe von Fossilien, und vergleichen es mit dem Alter der Wirtsinsekten, um für evolutionäre Zusammenhänge zu testen.
Lay summary

Inhalt und Ziele

Parasitoide unterscheiden sich von Parasiten dadurch, dass sie den Wirt obligatorisch abtöten. Sie entwickeln sich auf den Larven von anderen Insekten und Spinnen und spielen somit eine Schlüsselrolle in den meisten Ökosystemen. Dieses Projekt befasst sich mit den Pimpliformes, einer Gruppe mit mehr als 2,800 beschriebenen Arten in über 170 Gattungen. Mittels neuer DNA-Sequenziermethoden werden wir den ersten molekulären Stammbaum der Gruppe erstellen. Ca. 50 Fossilien, die ältesten aus der Kreidezeit, liefern Altersschätzungen, die uns helfen zu verstehen, inwiefern Artbildung und Aussterben durch Wirtswechsel oder durch neue Parasitierungsstrategien beeinflusst wurden.

Wissenschaftlicher und gesellschaftlicher Kontext

Diese Studie schliesst eine grosse Lücke in unserem Verständnis, wieso gewisse Gruppen artenreicher sind als andere, und in welchen evolutionären Zeiträumen Parasitoide sich an neu erscheinende oder verschwindende Wirtsgruppen anpassen können. Dies hat potentielle Konsequenzen im Artenschutz, aber auch für die Risikoabschätzung in der biologischen Schädlingsbekämpfung, in der zahlreiche Schlupfwespen eingesetzt werden.

Direct link to Lay Summary Last update: 13.01.2015

Responsible applicant and co-applicants

Employees

Publications

Publication
Illustrating phylogenetic placement of fossils using RoguePlots: An example from ichneumonid parasitoid wasps (Hymenoptera, Ichneumonidae) and an extensive morphological matrix
Klopfstein Seraina, Spasojevic Tamara (2019), Illustrating phylogenetic placement of fossils using RoguePlots: An example from ichneumonid parasitoid wasps (Hymenoptera, Ichneumonidae) and an extensive morphological matrix, in PLOS ONE, 14(4), e0212942-e0212942.
Hybrid capture data unravels a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes)
Klopfstein Seraina, Langille Barabara, Spasojevic Tamara, Broad Gavin R., Cooper Steven J.B., Austin Andrew D., Niehuis Oliver (2019), Hybrid capture data unravels a rapid radiation of pimpliform parasitoid wasps (Hymenoptera: Ichneumonidae: Pimpliformes), in Systematic Entomology, 44, 361-383.
One generalist or several specialist species? Wide host range and diverse manipulations of the hosts’ web-building behaviour in the true spider parasitoid Zatypota kauros (Hymenoptera: Ichneumonidae)
Korenko Stanislav, Spasojevic Tamara, Pekár Stano, Walter Gimme H., Korenková Vlasta, Hamouzová Kateřina, Kolářová Michaela, Kysilková Kristýna, Klopfstein Seraina (2018), One generalist or several specialist species? Wide host range and diverse manipulations of the hosts’ web-building behaviour in the true spider parasitoid Zatypota kauros (Hymenoptera: Ichneumonidae), in Insect Conservation and Diversity, 11(6), 587-599.
New ichneumonids (Hymenoptera, Ichneumonidae) from the Eocene Tadushi Formation, Russian Far East
KOPYLOV D.S., SPASOJEVIC T., KLOPFSTEIN S. (2018), New ichneumonids (Hymenoptera, Ichneumonidae) from the Eocene Tadushi Formation, Russian Far East, in Zootaxa, 4442(2), 319-330.
Seven remarkable new fossil species of parasitoid wasps (Hymenoptera, Ichneumonidae) from the Eocene Messel Pit
Spasojevic Tamara, Wedmann Sonja, Klopfstein Seraina (2018), Seven remarkable new fossil species of parasitoid wasps (Hymenoptera, Ichneumonidae) from the Eocene Messel Pit, in PLOS ONE, 13(6), e0197477-e0197477.
Ichneumonid parasitoid wasps from the Early Eocene Green River Formation: five new species and a revision of the known fauna (Hymenoptera, Ichneumonidae)
Spasojevic Tamara, Broad Gavin R., Bennett Andrew M.R., Klopfstein Seraina (2018), Ichneumonid parasitoid wasps from the Early Eocene Green River Formation: five new species and a revision of the known fauna (Hymenoptera, Ichneumonidae), in Paläontologische Zeitschrift, (1), 35-63.
More on the best evolutionary rate for phylogenetic analysis
Klopfstein Seraina, Massingham Tim, Goldman Nick (2017), More on the best evolutionary rate for phylogenetic analysis, in Systematic Biology, 66(5), 769-785.
Post-Eocene climate change across continental Australia and the diversification of Australasian spiny trapdoor spiders (Idiopidae: Arbanitinae)
Rix Mike, Cooper Steve J.B., Meusemann Karen, Klopfstein Seraina, Harrison Sophie E., Harvey Mark S., Austin Andrew D. (2017), Post-Eocene climate change across continental Australia and the diversification of Australasian spiny trapdoor spiders (Idiopidae: Arbanitinae), in Molecular Phylogenetics and Evolution, 109, 302-320.
Total-Evidence Dating under the Fossilized Birth–Death Process
Zhang Chi, Stadler Tanja, Klopfstein Seraina, Heath Tracy A., Ronquist Fredrik (2016), Total-Evidence Dating under the Fossilized Birth–Death Process, in Systematic Biology, 65(2), 228-249.
Molecular and morphological phylogenetics of chelonine parasitoid wasps (Hymenoptera: Braconidae), with a critical assessment of divergence time estimations
Kittel Rebecca, Austin Andrew, Klopfstein Seraina (2016), Molecular and morphological phylogenetics of chelonine parasitoid wasps (Hymenoptera: Braconidae), with a critical assessment of divergence time estimations, in Molecular Phylogenetics and Evolution, 224-241.
Total-Evidence Dating under the Fossilized Birth-Death Process
Zhang Chi, Stadler Tanja, Klopfstein Seraina, Heath Tracy, Ronquist Fredrik (2016), Total-Evidence Dating under the Fossilized Birth-Death Process, in Systematic Biology, 65(2), 228-249.
A Nonstationary Markov Model Detects Directional Evolution in Hymenopteran Morphology
Klopfstein Seraina, Vilhelmsen Lars, Ronquist Fredrik (2015), A Nonstationary Markov Model Detects Directional Evolution in Hymenopteran Morphology, in Systematic Biology, 64(6), 1089-1103.
Evolutionary history of the Hymenoptera
Peters Ralph S., Krogmann Lars, Mayer Christoph, Donath A., Gunkel S., Meusemann Karen, Kozlov A., Podsiadlowski L., Petersen Malte, Lanfear M:, Diez P.A., Heraty J., Kjer K.M., Klopfstein S., Meier R., Polidori C., Schmitt T., Liu S., Zhou X., Wappler T., Rust J., Misof B., Niehuis O., Evolutionary history of the Hymenoptera, in Current Biology, 27.

Datasets

Morphological matrix in NEXUS format

Author Klopfstein, Seraina; Spasojevic, Tamara
Publication date 07.11.2018
Persistent Identifier (PID) https://doi.org/10.5281/zenodo.1479740
Repository Zenodo
Abstract
Morphological matrix in NEXUS format - from Klopfstein & Spasejovic, Illustrating phylogenetic placement of fossils using RoguePlots: An example from ichneumonid parasitoid wasps (Hymenoptera, Ichneumonidae) and an extensive morphological matrix.Associated with a publication in preprint at bioRxiv: doi: https://doi.org/10.1101/425090

Collaboration

Group / person Country
Types of collaboration
Prof. Ilari Sääksjärvi, University of Turku Finland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Prof. Lars Vilhelmsen, Natural History Museum of Denmark Denmark (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Stanislav Korenko, Czech University of Life Sciences Prague Czech Republic (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Fredrik Ronquist, BIO, Naturhistoriska Riksmuseet Sweden (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Andrew Austin, The University of Adelaide Australia (Oceania)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Andrew Bennett, Canadian National Collection of Insects, Ottawa Canada (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Dmitry Kopylov, Russian Academy of Science Russia (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Gavin Broad, The Natural History Museum, London Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Sonja Wedemann, Senckenberg Frankfurt (Grube Messel) Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Evolution 2018 Montpellier: II Joint Congress on Evolutionary Biology Talk given at a conference Using fossils to date phylogenetic trees 19.08.2018 Montpellier, France Klopfstein Seraina;
Evolution 2018 Montpellier: II Joint Congress on Evolutionary Biology Talk given at a conference From the origin to the present: reconstructing and dating the tree of pimpliform parasitoid wasps 19.08.2018 Montpellier, France Spasojevic Tamara;
9th Congress of International Society of Hymenopterists ISH Talk given at a conference From the origin to the present: reconstructing and dating the tree of pimpliform parasitoid wasps 23.07.2018 Matsuyama, Japan Spasojevic Tamara;
Scientific meeting of the Swiss Systematics Society (SSSDay17) Talk given at a conference How many genes do we need? Phylogenomics of pimpliform parasitoid wasps 10.11.2017 Fribourg, Switzerland Klopfstein Seraina;
Biosyst.EU conference Talk given at a conference Using fossils to date phylogenetic trees 15.08.2017 Göteborg, Sweden Klopfstein Seraina;
biology17 Talk given at a conference Leave no stone unturned: challenges in Ichneumonidae palaeontology (oral contribution) 02.02.2017 Bern, Switzerland Spasojevic Tamara;
Scientific meeting of the Swiss Systematics Society (SSSDay16) Talk given at a conference Leave no stone unturned: challenges in Ichneumonidae palaeontology 10.11.2016 Sion, Switzerland Spasojevic Tamara;
biology16 Poster The hunt for the optimal rate 11.02.2016 Lausanne, Switzerland Klopfstein Seraina;
ПАЛЕОЭНТОМОЛОГИЯ: СООБЩЕСТВА И КРИЗИСЫ (Russian Palaeontology meeting) Talk given at a conference A total-evidence approach to dating with fossils 11.11.2015 Moscow, Russia Klopfstein Seraina;
Systematics Association Biennial Conference Talk given at a conference A total-evidence approach to dating with fossils 26.08.2015 Oxford, Great Britain and Northern Ireland Klopfstein Seraina;


Self-organised

Title Date Place

Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Vortrag an Museumsnacht NMBE: Rätselraten um ausgestorbene Schlupfwespen German-speaking Switzerland 2018

Associated projects

Number Title Start Funding scheme
194088 True or False? Fantastic Stories from our Natural History Collections 01.07.2020 Agora
192544 Total-evidence dating and the impact of the K-Pg mass extinction on Darwin wasps 01.09.2020 Project funding (Div. I-III)

Abstract

Parasitoid wasps constitute 4.5% of all described multicellular organisms, a number that is certainly an underestimate given that they are among the most poorly studied taxa (for comparison, all vertebrates together are only about 2.7%). They feed internally (endoparasitoids) or externally (ectoparasitoids) on immature or adult stages of other insects or arachnids to complete their larval development. Being at the top of the food web, parasitoids play a key role in almost every terrestrial ecosystem, and numerous species are successfully used in the biological control of pest insects. Their extraordinary species richness is often attributed to the parasitic lifestyle; however, our understanding of the relationships between parasitoid life history and rates of diversification is severely hampered by the lack of basic knowledge of species numbers, phylogenetic relationships, host ranges, and the evolutionary ages of the different groups.In this project, we aim to reconstruct the phylogeny of the Pimpliformes (Hymenoptera, Ichneumonidae), a monophyletic assemblage exhibiting various types of parasitoid lifestyles and attacking a range of different host groups. The only available molecular phylogeny is based on a single gene and a limited number of taxa; many of the deeper splits remain unresolved. We aim to sequence the transcriptomes of ten pimpliforme species to obtain a set of candidate markers for phylogenetics. The hundreds of orthologous single-copy genes thus identified are going to be analysed for their predicted phylogenetic informativeness, and a set of 10-20 genes will be chosen for amplification in a densely sampled set of about 300 taxa representing almost all pimpliforme genera.In the next step, we will obtain a dated phylogeny using an approach recently co-developed by the main applicant and termed “total-evidence dating”. This approach treats fossils as terminals instead of relying on secondary interpretations of the fossil record. For fossils to be included, we need to score morphological characters for both extant and fossil taxa into a comprehensive matrix, which will be analysed along-side the molecular data to obtain robust divergence time estimates. Based on the obtained dated tree, we will investigate patterns of diversification in Pimpliformes. In a first step, we are going to conduct the first stochastic biogeographic analysis of this group in a phylogenetic context, and compare paleogeography to divergence times to assess the impact of vicariance and dispersal on the diversification of Pimpliformes. In a second step, we will reconstruct the evolution of host ranges and parasitoid life history strategies using stochastic models of character evolution. And finally, we are going to look for correlations between parasitoid strategies and diversification rates, e.g., examining whether the more specialised endoparasitoids radiated more rapidly than the comparatively generalist ectoparasitoids.
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