bioelectrochemistry; LCP crystallization; molecular transport; membrane proteins; lipid mesophases; lyotropic liquid crystals; lipid synthesis; small angle X-ray scattering
Zabara Alexandru, Chong Josephine Tse Yin, Martiel Isabelle, Stark Laura, Cromer Brett A., Speziale Chiara, Drummond Calum John, Mezzenga Raffaele (2018), Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains, in NATURE COMMUNICATIONS
, 9, 544.
Duss Michael, Manni Livia Salvati, Moser Laurent, Handschin Stephan, Mezzenga Raffaele, Jessen Henning J., Landau Ehud M. (2018), Lipidic Mesophases as Novel Nanoreactor Scaffolds for Organocatalysts: Heterogeneously Catalyzed Asymmetric Aldol Reactions in Confined Water, in ACS APPLIED MATERIALS & INTERFACES
, 10(5), 5114-5124.
Zabara Alexandru, Meikle Thomas G., Trenker Raphael, Yao Shenggen, Newman Janet, Peat Thomas S., Separovic Frances, Conn Charlotte E., Call Melissa J., Call Matthew E., Landau Ehud M., Drummond Calum J. (2017), Lipidic Cubic Phase-Induced Membrane Protein Crystallization: Interplay Between Lipid Molecular Structure, Mesophase Structure and Properties, and Crystallogenesis, in CRYSTAL GROWTH & DESIGN
, 17(11), 5667-5674.
Nazaruk Ewa, Górecka Ewa, Osornio Yazmin M., Landau Ehud M., Bilewicz Renata (2017), Charged additives modify drug release rates from lipidic cubic phase carriers by modulating electrostatic interactions, in Journal of Electroanalytical Chemistry
Speziale Chiara, Zabara Alexandru Florian, Drummond Calum John, Mezzenga Raffaele (2017), Active Gating, Molecular Pumping, and Turnover Determination in Biomimetic Lipidic Cubic Mesophases with Reconstituted Membrane Proteins, in ACS Nano
, 11(11), 11687-11693.
Szlezak Monika, Nieciecka Dorota, Joniec Aleksandra, Pękała Marek, Gorecka Ewa, Emo Mélanie, Stébé Marie J., Krysiński Paweł, Bilewicz Renata (2017), Monoolein Cubic Phase Gels and Cubosomes Doped with Magnetic Nanoparticles–Hybrid Materials for Controlled Drug Release, in ACS Applied Materials & Interfaces
, 9(3), 2796-2805.
Speziale Chiara (2016), A macroscopic H+ and Cl- ions pump via reconstitution of EcClC membrane proteins in lipidic cubic mesophases, in Proc. Natl. Acad. Sci. USA
, 113, 7491.
Jablonowska Elżbieta (2016), Interactions of lipidic cubic phase nanoparticles with lipid membranes, in Langmuir
, 32, 9640.
Aleandri Simone, Bandera Davide, Mezzenga Raffaele, Landau Ehud M. (2015), Biotinylated Cubosomes: A Versatile Tool for Active Targeting and Code livery of Paclitaxel and a Fluorescein-Based Lipid Dye, in LANGMUIR
, 31(46), 12770-12776.
Nazaruk Ewa, Miszta Przemyslaw, Filipek Slawomir, Gorecka Ewa, Landau Ehud M., Bilewicz Renata (2015), Lyotropic Cubic Phases for Drug Delivery: Diffusion and Sustained Release from the Mesophase Evaluated by Electrochemical Methods, in LANGMUIR
, 31(46), 12753-12761.
Matyszewska Dorota, Bilewicz Renata, Su ZhangFei, Abbasi Fatemeh, Leitch J Jay, Lipkowski Jacek, PM-IRRAS studies of DMPC bilayers supported on Au(111) electrodes modified with hydrophilic monolayers of thioglucose., in Langmuir : the ACS journal of surfaces and colloids
Membranes are ubiquitous to all living systems, and progress in membrane biology, specifically membrane structural biology, has witnessed enormous advances in the past years. The classical fluid mosaic model of a membrane, in which hydrophobic integral components such as lipids and membrane proteins are constrained within the plane of the membrane, but are free to diffuse laterally, has been constantly refined, and knowledge and understanding of the structure, function and dynamics of various membrane subsystems is now available at atomic resolution. Whereas production of well-diffracting crystals of membrane protein was considered the most difficult hurdle in molecular membrane biology only two decades ago, conceptual and technological advances in recent years have resulted in a remarkable increase of available high resolution structures of membrane proteins and their complexes, with a concomitant deeper understanding of their function, dynamics and assembly. Among those advances, the concept of utilizing the distinct structural and dynamic material properties of lipidic cubic phases (LCPs) for the solubilization, stabilization and crystallization of membrane proteins, which was introduced by the UZH co-applicant of this proposal yielded about 10% of all membrane protein structures currently available, and all but one of the structures of the pharmacologically important G protein-coupled receptors (GPCRs). Intriguingly, this significant achievement was accomplished using but a few related monoacylglycerols whose chemical structure and function, as well as the ensuing phase behavior, are very similar. Moreover, despite the increased importance of LCPs in membrane studies, the molecular mechanisms underlying LCP-based crystallization, and the underlying molecular interactions between lipids and proteins in such mesophases, are still unclear.Progress in fundamental as well as applied aspects of membrane biology and biophysics thus calls for the design, synthesis and characterization of novel lipids and the judicious use of ensuing lipidic nanomaterials. Advances in this area, which lies at the interface between biophysical chemistry, soft condensed matter physics and technology, and surface and colloidal chemistry, will best be facilitated by an interdisciplinary approach. The current application brings together experts in the fields of lipid design and synthesis, membrane structural biology, soft condensed matter physics, drug delivery, electrochemistry, and surface chemistry, who are committed to address a number of inter-related issues. These include the mechanisms of membrane protein reconstitution, orientation and crystallization within lipidic mesophases; fundamental aspects of transport and dynamics in mesophases, which will lead to applications in drug delivery; and novel stimuli-responsive mesoscopic matrices.Elucidating these fundamental issues will enhance our understanding of the complex structural and dynamic interactions between membrane proteins and lipids within mesoscopic biomaterials, and may have relevance in biomedical science and technology, pharmaceutical science and even food technology.