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Recombinant, Photosynthetic Bacteria for Photovoltaic Solar Energy Conversion

Applicant Boghossian Ardemis
Number 154269
Funding scheme Assistant Professor (AP) Energy Grants
Research institution Institut des sciences et ingénierie chimiques EPFL - SB - ISIC
Institution of higher education EPF Lausanne - EPFL
Main discipline Chemical Engineering
Start/End 01.09.2015 - 31.12.2019
Approved amount 1'165'828.00
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All Disciplines (3)

Discipline
Chemical Engineering
Electrical Engineering
Material Sciences

Keywords (13)

mtrABC; Shewanella oneidensis MR-1; photovoltaics; protein engineering; electron conduit; cyanobacteria; photosynthesis; mtrA; mtrB; mtrC; multiheme cytochrome c; extracellular electron transfer; cytochrome c machinery (ccm)

Lay Summary (French)

Lead
Les systèmes photovoltaïques à matériaux biologiques renouvelables présentent de nombreux avantages en matière de stockage d’énergie, durabilité, séquestration de CO2, et abondance de matières premières, avec lesquels aucune autre technologie existante ne peut rivaliser. Malheureusement, le développement de ces biotechnologies prometteuses en dehors des laboratoires de recherche est actuellement limité à cause de leur faible rendement énergétique. Bien que les organismes vivants soient capables de capter l’énergie solaire avec des efficacités comparables à celles de cellules photovoltaïques commerciales, l’inefficacité du mécanisme d’extraction d’énergie de ces organismes limite actuellement leur application industrielle.
Lay summary
Contenu et objectifs du travail de recherche
Le but de ce projet et de développer un organisme photosynthétique recombinant, qui soit conçu spécifiquement afin d’exporter l’énergie solaire à travers un conduit électronique à base protéinique. Les objectifs spécifiques sont (i) de concevoir des plateformes de production de protéine à hème synthétique par l’intermédiaire de cyanobactéries, (ii) d’optimiser l’interface entre le système photosynthétique des cyanobactéries et le conduit à électrons extracellulaire, (iii) de comprendre et quantifier les mécanismes fondamentaux, et les contraintes de développement de ce nouveau système recombinant, à l’aide d’un modèle physique élaboré et de simulations du métabolisme.

Contexte scientifique et social du projet de recherche
Ce travail de recherche se place dans le contexte du grand défi scientifique que pose la production durable d’énergie au niveau mondial, en proposant une approche nouvelle, et potentiellement bénéfique, aux techniques de captation d’énergie solaire. Le modèle scientifique et les résultats obtenus seront également une ressource pour la communauté scientifique, afin de mieux comprendre les mécanismes fondamentaux et les contraintes associées au développement de systèmes recombinants, qui sont l’objet d’un intérêt grandissant.




Direct link to Lay Summary Last update: 11.12.2014

Responsible applicant and co-applicants

Employees

Publications

Publication
Templating colloidal sieves for tuning nanotube surface interactions and optical sensor responses
Gillen Alice J., Siefman Daniel J., Wu Shang-Jung, Bourmaud Claire, Lambert Benjamin, Boghossian Ardemis A. (2020), Templating colloidal sieves for tuning nanotube surface interactions and optical sensor responses, in Journal of Colloid and Interface Science, 565, 55-62.
Design of Optimized PEDOT‐Based Electrodes for Enhancing Performance of Living Photovoltaics Based on Phototropic Bacteria
Reggente Melania, Politi Sara, Antonucci Alessandra, Tamburri Emanuela, Boghossian Ardemis A. (2020), Design of Optimized PEDOT‐Based Electrodes for Enhancing Performance of Living Photovoltaics Based on Phototropic Bacteria, in Advanced Materials Technologies, 5(3), 1900931-1900931.
Analytical Approaches for Monitoring DNA–Protein Interactions
Wu Shang-Jung, Boghossian Ardemis A. (2019), Analytical Approaches for Monitoring DNA–Protein Interactions, in CHIMIA International Journal for Chemistry, 73(4), 283-287.
Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)
Zubkovs Vitalijs, Antonucci Alessandra, Schuergers Nils, Lambert Benjamin, Latini Andrea, Ceccarelli Raino, Santinelli Andrea, Rogov Andrii, Ciepielewski Daniel, Boghossian Ardemis A. (2018), Spinning-disc confocal microscopy in the second near-infrared window (NIR-II), in Scientific Reports, 8(1), 13770-13770.
Xeno Nucleic Acid Nanosensors for Enhanced Stability Against Ion-Induced Perturbations
Gillen Alice J., Kupis-Rozmysłowicz Justyna, Gigli Carlo, Schuergers Nils, Boghossian Ardemis A. (2018), Xeno Nucleic Acid Nanosensors for Enhanced Stability Against Ion-Induced Perturbations, in The Journal of Physical Chemistry Letters, 9(15), 4336-4343.
Restriction Enzyme Analysis of Double-Stranded DNA on Pristine Single-Walled Carbon Nanotubes
Wu Shang-Jung, Schuergers Nils, Lin Kun-Han, Gillen Alice, Corminboeuf Clemence, Boghossian Ardemis (2018), Restriction Enzyme Analysis of Double-Stranded DNA on Pristine Single-Walled Carbon Nanotubes, in ACS Applied Materials & Interfaces, 37386.
Mediatorless, Reversible Optical Nanosensor Enabled through Enzymatic Pocket Doping
Zubkovs Vitalijs, Schuergers Nils, Lambert Benjamin, Ahunbay Esra, Boghossian Ardemis A. (2017), Mediatorless, Reversible Optical Nanosensor Enabled through Enzymatic Pocket Doping, in Small, 13(42), 1701654-1701654.
A synthetic biology approach to engineering living photovoltaics
Schuergers Nils, Werlang Caroline, Ajo-Franklin Caroline, Boghossian Ardemis (2017), A synthetic biology approach to engineering living photovoltaics, Ecole Polytechnique Federale de Lausanne (EPFL), Energy & Environmental Science.
Noncovalent Protein and Peptide Functionalization of Single-Walled Carbon Nanotubes for Biodelivery and Optical Sensing Applications
Antonucci Alessandra, Kupis-Rozmysłowicz Justyna, Boghossian Ardemis A. (2017), Noncovalent Protein and Peptide Functionalization of Single-Walled Carbon Nanotubes for Biodelivery and Optical Sensing Applications, in ACS Applied Materials & Interfaces, 9(13), 11321-11331.

Collaboration

Group / person Country
Types of collaboration
Elisabetta Carata, University of Salento Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Emanuela Tamburri, Università degli Studi di Roma “Tor Vergata” Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel
Swagatika Sahoo, Indian Institute of Technology Madras IIT-M India (Asia)
- in-depth/constructive exchanges on approaches, methods or results
Raghunathan Rengaswamy, Indian Institute of Technology Madras IIT-M India (Asia)
- in-depth/constructive exchanges on approaches, methods or results
Luciana Dini, Sapienza University of Rome Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Caroline Ajo-Franklin, Molecular Foundry, Lawrence Berkeley National Laboratory (LBNL) United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Aravind Chandiran, Indian Institute of Technology Madras IIT-M India (Asia)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Materials Research Society (MRS) Talk given at a conference A NanoBioengineering Approach to Developing Living Photovoltaics 01.12.2019 Boston, United States of America Boghossian Ardemis;
American Institute of Chemical Engineers (AIChE) Poster Near-Infrared Confocal Imaging of Single-Walled Carbon Nanotube Uptake in Bacterial Cells 10.11.2019 Orlando, United States of America Schürgers Nils; Boghossian Ardemis; Antonucci Alessandra;
e-Conversion Conference Talk given at a conference A NanoBioengineering Approach to Developing Living Photovoltaics 09.09.2019 Venice, Italy Boghossian Ardemis;
Electrochemical Society (ECS) Talk given at a conference Real-Time Near-Infrared Confocal Imaging of Bacterial Cell Division in the Presence of Single-Walled Carbon Nanotubes 26.05.2019 Dallas, United States of America Antonucci Alessandra; Schürgers Nils; Boghossian Ardemis;
Electrochemical Society (ECS) Talk given at a conference Near-Infrared Confocal Imaging of Single-Walled Carbon Nanotube Uptake in Bacterial Cells 26.05.2019 Dallas, United States of America Antonucci Alessandra; Boghossian Ardemis; Schürgers Nils;
Electrochemical Society (ECS) Talk given at a conference A Nanobioengineering Frontier for Next-Generation Optical Devices 26.05.2019 Dallas, United States of America Boghossian Ardemis;
American Institute of Chemical Engineers (AIChE) Poster Near-Infrared Confocal Imaging of Single-Walled Carbon Nanotube Uptake in Bacteria 28.10.2018 Pittsburgh, United States of America Schürgers Nils; Antonucci Alessandra; Boghossian Ardemis;
Swiss Chemical Society (SCS) Poster Near-Infrared Confocal Imaging of Single-Walled Carbon Nanotube Uptake in Nanobionic Bacterial Cells 07.09.2018 Lausanne, Switzerland Boghossian Ardemis; Schürgers Nils; Antonucci Alessandra;
American Institute of Chemical Engineers (AIChE) Individual talk Interaction of Single-walled Carbon Nanotubes (SWCNTs) with Photosynthetic Organisms 29.10.2017 Minneapolis, United States of America Schürgers Nils; Boghossian Ardemis; Antonucci Alessandra;
231st Electrochemical Society (ECS) Meeting Talk given at a conference Interaction of Single-Walled Carbon Nanotubes (SWCNTs) with Photosynthetic Organisms 28.05.2017 New Orleans, United States of America Schürgers Nils; Antonucci Alessandra; Boghossian Ardemis;
Electrochemical Society (ECS) Individual talk Interaction of single-walled carbon nanotubes (SWCNTs) with photosynthetic organisms 28.05.2017 New Orleans, United States of America Schürgers Nils; Antonucci Alessandra; Boghossian Ardemis;
Trends in Nanotechnology International Conference (TNT 2016) Poster Interaction of single-walled carbon nanotubes (SWCNTs) with photosynthetic systems 05.09.2016 Fribourg, Switzerland Boghossian Ardemis; Antonucci Alessandra; Schürgers Nils;
MP-EPFL Bioinspired Systems Poster Single-walled carbon nanotube uptake in cyanobacteria 13.07.2016 Berlin, Germany Antonucci Alessandra;
MPG-EPFL Bioinspired Systems Talk given at a conference A NanoBioengineering Approach to Developing Living Photovoltaics 13.07.2016 Berlin, Germany Boghossian Ardemis;
Bioinspired nanosystems for energy conversion Talk given at a conference A nanobioengineering approach to developing living photovoltaics 11.07.2016 Berlin, Germany Boghossian Ardemis;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Lab tour for young girls (11-13 years old) interested in science Performances, exhibitions (e.g. for education institutions) 15.07.2019 Lausanne, Switzerland Reggente Melania;
Experimental Demonstration on Rouge FM Performances, exhibitions (e.g. for education institutions) 19.06.2017 Lausanne, Switzerland Kupis-Rozmyslowicz Justyna;


Self-organised

Title Date Place
Buehler Research Overview 12.12.2019 Lausanne, Switzerland

Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Lab tour for young girls interested in science Western Switzerland 2019
Media relations: radio, television Live Radio Demonstration of "Living Photovoltaic" Rouge FM Western Switzerland 2017

Awards

Title Year
ECS travel award 2019
European Research Council (ERC) Starting Grant 2019
Young Investigator in Nano Research Award 2019
ECS travel award 2018
Frontiers in Chemistry "Rising Star" 2018
SCS poster award 2018
Chemistry Travel Award 2017
ECS Travel Award 2017
Keynote Address for AGBU Alex & Marie Manoogian School 2017
MPG-EPFL Student travel award 2016
Roger Taylor Award 2016
Trends in Nanotechnology (TNT) Travel Award 2016

Associated projects

Number Title Start Funding scheme
189631 Integrated Microscope for Live-Cell and Single-Molecule Imaging 01.01.2020 R'EQUIP

Abstract

Biological systems offer inherent advantages in fault tolerance, regeneration, abundance, and energy storage ideally suited for photovoltaic (PV) applications. Despite promising maximum theoretical efficiencies, biological PVs remain an underdeveloped technology hindered by poor electron extraction mechanisms that limit efficiencies. The focus of this proposal is to engineer a recombinant, light-harvesting organism capable of exporting photosynthetically derived electrons for solar energy use. The proposed approach is to couple the electron-exporting mechanism in Shewanella with the light-harvesting mechanism in cyanobacteria to create a recombinant, living cell capable of exporting electrons derived from absorbing light to split water. This approach entails the heterologous expression and optimization of the three proteins, mtrA, mtrB, and mtrC, responsible for exporting electrons in Shewanella in cyanobacteria. Through synthetic biology and protein engineering, this project focuses on engineering and optimizing the interface between the synthetic Shewanella electron conduit and the light-harvesting, cyanobacterial system.
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