biofilm; fermentation; lactic acid; carboxylic acids; lignocellulosic biomass; enzymatic hydrolysis; consolidated bioprocess
Yeap Jher Hau, Héroguel Florent, Shahab Robert L., Rozmysłowicz Bartosz, Studer Michael H., Luterbacher Jeremy S. (2018), Selectivity Control during the Single-Step Conversion of Aliphatic Carboxylic Acids to Linear Olefins, in ACS Catalysis
, 8(11), 10769-10773.
Shahab Robert L., Luterbacher Jeremy S., Brethauer Simone, Studer Michael H. (2018), Consolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal-bacterial consortium, in Biotechnology and Bioengineering
, 115(5), 1207-1215.
Xiros Charilaos, Studer Michael H. (2017), A Multispecies Fungal Biofilm Approach to Enhance the Celluloyltic Efficiency of Membrane Reactors for Consolidated Bioprocessing of Plant Biomass, in Frontiers in Microbiology
, 8, 1930.
Brethauer Simone, Studer Michael (2015), Biochemical Conversion Processes of Lignocellulosic Biomass to Fuels and Chemicals – A Review, in Chimia
, 69(10), 572-581.
In order to relieve the world’s dependence on fossil fuels, alternative sustainable sources for energy and chemicals must be exploited. To this end, biorefinery concepts are under development to integrate the production of fuels, power, materials and chemicals from lignocellulosic waste biomass. The aim of this project is to enhance the possible processing routes of such a future biorefinery by developing efficient biochemical conversion processes to intermediate products that can further be processed by chemocatalytic methods to diesel and jet fuel-like mixtures of alkanes and olefins. Specifically, we aim to produce lactic acid as well as less oxygenated C2 to C6 carboxylic acids from pretreated agricultural waste products by a highly efficient consolidated bioprocess employing the multispecies biofilm membrane (MBM) reactor developed in a previous SNSF Ambizione project. Briefly, the MBM process allows to combine the production of cellulolytic enzymes by a specialised aerobic fungal strain such as Trichoderma reesei, the hydrolysis of the cellulose to mono-sugars and the fermentation of the resulting sugars to e.g. ethanol by an anaerobic strain in one reactor. The targeted volatile fatty acids (VFAs) can be produced by a variety of microorganisms from different feedstocks. We will investigate and compare three different VFA production routes employing 1) a lactic acid production strain in combination with the rumen bacterium M. elsdenii that converts lactic acid to mixed VFAs, 2) the obligate anaerobic C. tyrobutyricum producing butyric acid and 3) an anaerobic undefined mixed consortium. Based on the results achieved and in close collaboration with the catalytic group (J. Luterbacher), the most promising combined reaction scheme to build the desired diesel and jet fuel molecules will be selected and further optimized.