Interneuron; Neuronal computation; Olfaction; Zebrafish; Optogenetics; Paleocortex; Memory; Olfactory cortex
Wanner Adrian A., Friedrich Rainer W. (2020), Whitening of odor representations by the wiring diagram of the olfactory bulb, in Nature Neuroscience
, 23(3), 433-442.
Huang Kuo-Hua, Rupprecht Peter, Frank Thomas, Kawakami Koichi, Bouwmeester Tewis, Friedrich Rainer W. (2020), A virtual reality system to analyze neural activity and behavior in adult zebrafish, in Nature Methods
, 17(3), 343-351.
Frank Thomas, Mönig Nila R., Satou Chie, Higashijima Shin-ichi, Friedrich Rainer W. (2019), Associative conditioning remaps odor representations and modifies inhibition in a higher olfactory brain area, in Nature Neuroscience
, 22(11), 1844-1856.
Namekawa Iori, Moenig Nila R., Friedrich Rainer W. (2018), Rapid olfactory discrimination learning in adult zebrafish, in Experimental Brain Research
, 236(11), 2959-2969.
Rupprecht Peter, Friedrich Rainer W. (2018), Precise Synaptic Balance in the Zebrafish Homolog of Olfactory Cortex, in Neuron
, 100(3), 669-683.e5.
Jacobson Gilad, Rupprecht Peter, Friedrich Rainer W. (2018), Experience-dependent plasticity of odor representations in the telencephalon of zebrafish, in Current Biology
, 28, 1-14.
Wanner Adrian A., Genoud Christel, Friedrich Rainer W. (2016), 3-dimensional electron microscopic imaging of the zebrafish olfactory bulb and dense reconstruction of neurons, in Scientific Data
, 3, 160100.
Wanner Adrian A., Genoud Christel, Masudi Tafheem, Siksou L., Friedrich Rainer W. (2016), Dense EM-based reconstruction of the interglomerular projectome in the zebrafish olfactory bulb, in Nature Neuroscience
, 19, 816-825.
Rupprecht Peter, Prendergast Andrew, Wyart Claire, Friedrich Rainer W. (2016), Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy, in Biomed. Optics Expr.
, 7(5), 1656-1671.
Background and general aim: The central nervous system of vertebrates contains a diverse spectrum of inhibitory interneurons that are associated with specific microcircuits. The goal of this project is to analyze the computational functions of such microcircuits by studying and manipulating defined interneurons. The project takes advantage of zebrafish as a small genetic vertebrate model and focuses on a higher olfactory brain area (Dp) that is homologous to paleocortex (olfactory cortex). Dp is thought to establish Gestalt representations of olfactory objects and to function as an auto-associative network that stores odor-encoding activity patterns in memory. We will analyze the elementary cortical computations underlying such higher brain functions by genetic targeting of defined interneurons, measurements of neuronal activity patterns, functional manipulations of defined interneurons by opto- and pharmacogenetics, and behavioral experiments. The following specific aims will be addressed:1.Elementary computations in olfactory cortex/Dp. Genetically defined interneurons will be characterized and manipulated in order to define the associated microcircuits, to examine their canonical computational functions, and to explore their role in olfactory processing. 2.Neuromodulation of microcircuits. Using optogenetic approaches we will examine how neuromodulatory inputs, particularly dopaminergic and cholinergic inputs, control the functions of microcircuits. We will specifically test the hypothesis that neuromodulation regulates inhibitory microcircuits to control neuronal plasticity and information storage in autoassociative networks. 3.Behavioral functions of elementary cortical computations. Opto- and pharmacogenetic manipulations of defined interneurons will be combined with behavioral assays to examine how elementary cortical computations influence behavior. We will specifically test the hypothesis that inhibitory microcircuits stabilize odor perception by stabilizing odor-encoding activity patterns.4.Abnormal microcircuit function in genetic disease models. We will explore the possibility to study dysfunctions of microcircuits in zebrafish carrying mutations that have been associated with specific diseases in humans.Expected value: We expect to uncover direct relationships between microcircuit structure, function and dysfunction that provide quantitative insights into elementary cortical computations. The combination of functional manipulations and behavioral experiments is expected to establish direct links between neuronal circuit function and defined behaviors. The general goal is to provide systematic and mechanistic insights into cortical computations underlying higher brain functions.