Phytophthora ; Chestnut blight; Epidemiology; Invasive forest pathogens; Biological control
Rigling Daniel, Prospero Simone (2018), Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control., in Molecular plant pathology
, 19, 7-12.
Nuskern Lucija, Ježić Marin, Liber Zlatko, Mlinarec Jelena, Ćurković Perica Mirna (2017), Cryphonectria hypovirus 1- Induced Epigenetic Changes in Infected Phytopathogenic Fungus Cryphonectria parasitica, in Microbial Ecology
Meyer Joana B, Trapiello Estefanía, Senn-Irlet Beatrice, Sieber Thomas N, Cornejo Carolina, Aghayeva Dilzara, González Ana J, Prospero Simone (2017), Phylogenetic and phenotypic characterisation of Sirococcus castaneae comb. nov. (synonym Diplodina castaneae), a fungal endophyte of European chestnut., in Fungal biology
, 121(8), 625-637.
Poljak Igor, Idžojtić Marilena, Šatović Zlatko, Ježić Marin, Ćurković Perica Mirna, Simovski Bojan, Acevski Jane, Liber Zlatko (2017), Genetic diversity of the sweet chestnut (Castanea sativa Mill.) in central Europe and the western part of the Balkan Peninsula, and evidence of marron genotype introgression into wild populations, in Tree Genetics and Genomes
, 13(1), 18.
Krstin Ljiljana, Katanić Zorana, Ježić Marin, Poljak Igor, Nuskern Lucija, Matković Ivana, Idžojtić Marilena, Ćurković-Perica Mirna (2017), Biological control of chestnut blight in Croatia: an interaction between host sweet chestnut, its pathogen Cryphonectria parasitica and the biocontrol agent Cryphonectria hypovirus 1, in Pest management science
, 73(3), 582-589.
Supatashvili Archil, Tavadze Bidzina, Rigling Daniel, Sotirovski Kiril (2017), Chestnut blight (Cryphonectria parasitica) and perspective for biocontrol of the disease with hypovirulence, in Goncharvov N. (ed.), 274-277.
Aghayeva Dilzara, Rigling Daniel, Prospero Simone (2017), Low genetic diversequent sexual reproduction of the chestnut blight fungus Cryphonectria parasitica in Azerbaijan, in Forest Pathology
, 47:e12357, 1-7.
Mlinarec Jelena, Nuskern Lucija, Jezic Marin, Rigling Daniel, Curkovic-Perica Mirna (2017), Molecular evolution and invasion pattern of Cryphonectria hypovirus 1 in Europe: Mutation rate, and selection pressure differ between genome domains, in Virology
, 514, 156-164.
Forests and natural ecosystems are increasingly threatened by invasive pests and pathogens that are spread by trade of plants and plant material (e.g. wood). In this proposal, we will tackle two invasive diseases that affect European chestnut trees (Castanea sativa). Chestnut is an important multipurpose tree species in many rural areas. Fruits are used for food - directly or manufactured, as well as for feeding livestock, while wood is used for fuel, timber, poles, stakes, and as a natural source of tannins. Ink disease is caused by two soil-borne Phytophthora species (P. cinnamomi, P. cambivora) that attack the roots of chestnut trees, but also of other plant species, causing severe decline and mortality. The distribution and impact of the two pathogens in chestnut forests in the Balkans and Georgia is largely unknown. We will conduct intensive surveys and samplings in symptomatic and asymptomatic chestnut stands to determine the incidence and impact of these two pathogens. In addition, we will determine which other Phytophthora species are present in these chestnut ecosystems. Isolations will be done from symptomatic tissue and soil samples using baiting methods and Phytophthora isolates will be identified using morphological and molecular methods.Chestnut blight caused by the ascomycete fungus Cryphonectria parasitica is the second invasive disease that will be studied. The research on this disease will be a continuation of previous collaborations and will include an in depth analysis of the clonal chestnut blight epidemic in Eastern Europe. We will use next-generation sequencing technology to analyse single nucleotide polymorphisms (SNP), which will be used to reconstruct the invasion history of a single C. parasitica genotype that spread across south-eastern Europe. Furthermore, we will test the hypothesis that this genotype spread because it has a greater fitness or virulence compared to other genotypes found in Europe (clonal selection hypothesis). In addition, we will characterize novel Cryphonectria hypoviruses that we previously detected in Georgia and determine their potential for biological control of chestnut blight. Finally, we will conduct a common field experiment in all partner countries to evaluate the efficiency of biological control with natural hypovirulence. We hypothesize that (1) naturally occurring hypoviruses will infect artificially initiated cankers, (2) the infection rates correlates with the incidence of the hypovirus in the population, and (3) fungal vegetative incompatibility barriers significantly reduce hypovirus infection.