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Understanding Dendritic Cell Development and Function

English title Understanding Dendritic Cell Development and Function
Applicant Tussiwand Roxane
Number 150714
Funding scheme SNSF Professorships
Research institution Departement Biomedizin Universität Basel
Institution of higher education University of Basel - BS
Main discipline Immunology, Immunopathology
Start/End 01.10.2014 - 30.09.2018
Approved amount 1'489'124.00
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All Disciplines (2)

Discipline
Immunology, Immunopathology
Molecular Biology

Keywords (4)

Transcription factors; Function; Development; Dendritic cells

Lay Summary (Italian)

Lead
Le cellule dendritiche sono essenziali nella regolazione della risposta immunitaria. Capire il loro sviluppo e il loro funzionamento è fondamentale per capire il nostro sistema immunitario.
Lay summary

Nel 1973 Ralph M. Steinman e Zanvil A. Cohn hanno identificato delle cellule caratterizzate da una morfologia stellata, capaci di stimolare la risposta immunitaria. Questa scoperta ha dato origine al nome di “cellule dendritiche”, ed all’assegnazione del Premio Nobel nel 2011 a R. M. Steinman. Oggi sappiamo che le cellule dendritiche, sono distribuite nella maggior parte dei tessuti e possono essere considerate come guardiani della risposta immunitaria. Equipaggiate di speciali sensori, queste cellule scrutano l’ambiente circostante alla costante ricerca di microrganismi.  In caso d’infezione, le cellule dendritiche si attivano e stimolano la risposta immunitaria, mentre in assenza d’infezione, mantengono sotto controllo il sistema immunitario, preservando cosi l’integrità dei tessuti. Capire il funzionamento delle cellule dendritiche equivale a capire come il delicato equilibrio tra induzione o repressione della risposta immunitaria è regolato. Il seguente progetto di ricerca analizza questa domanda sotto l’aspetto molecolare; partendo dall’idea che la regolazione molecolare di una cellula ne rifletta il comportamento evolutivo e funzionale. 

 




Direct link to Lay Summary Last update: 19.01.2015

Responsible applicant and co-applicants

Name Institute

Employees

Publications

Publication
Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells
Rodrigues Patrick Fernandes, Alberti-Servera Llucia, Eremin Anna, Grajales-Reyes Gary E., Ivanek Robert, Tussiwand Roxane (2018), Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells, in Nature Immunology, 19(7), 711-722.
Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection
Sparber Florian, Dolowschiak Tamas, Mertens Sarah, Lauener Laura, Clausen Björn E., Joller Nicole, Stoitzner Patrizia, Tussiwand Roxane, LeibundGut-Landmann Salomé (2018), Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection, in PLOS Pathogens, 14(5), e1007069-e1007069.
Effective Labeling of Primary Somatic Stem Cells with BaTiO 3 Nanocrystals for Second Harmonic Generation Imaging
Sugiyama Nami, Sonay Ali Y., Tussiwand Roxanne, Cohen Bruce E., Pantazis Periklis (2018), Effective Labeling of Primary Somatic Stem Cells with BaTiO 3 Nanocrystals for Second Harmonic Generation Imaging, in Small, 14(8), 1703386-1703386.
Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF–IRF4 transcription factor complex
Iwata Arifumi, Durai Vivek, Tussiwand Roxane, Briseño Carlos G, Wu Xiaodi, Grajales-Reyes Gary E, Egawa Takeshi, Murphy Theresa L, Murphy Kenneth M (2017), Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF–IRF4 transcription factor complex, in Nature Immunology, 18(5), 563-572.
Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex
Iwata A, Durai V, Tussiwand R, Briseno CG, Wu X, Grajales-Reyes GE, Egawa T, Murphy TM, Murphy KM (2017), Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex, in Nature Immunology, 18(5), 563-72.
Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and monocyte fate
Wu X, Briseno CG, Grajales-Reyes GE, Haldar M, Iwata A, Kretzer NM, KC W, Tussiwand R, Higashi Y, Murphy TM, Murphy KM (2016), Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and monocyte fate, in PNAS, 113(51), 14775-14780.
Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and monocyte fate
Wu Xiaodi, Briseño Carlos G., Grajales-Reyes Gary E., Haldar Malay, Iwata Arifumi, Kretzer Nicole M., KC Wumesh, Tussiwand Roxane, Higashi Yujiro, Murphy Theresa L., Murphy Kenneth M. (2016), Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and monocyte fate, in Proceedings of the National Academy of Sciences, 113(51), 14775-14780.
RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells.
Kretzer NM, Theisen DJ, Tussiwand R, Briseño CG, Grajales-Reyes GE, Wu X, Durai V, Albring J, Bagadia P, Murphy TL, Murphy KM (2016), RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells., in Journal of Experimental Medicine, 2013(13), 2871-2883.
Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12.
Everts Bart, Tussiwand Roxane, Dreesen Leentje, Fairfax Keke C, Huang Stanley Ching-Cheng, Smith Amber M, O'Neill Christina M, Lam Wing Y, Edelson Brian T, Urban Joseph F, Murphy Kenneth M, Pearce Edward J (2016), Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12., in The Journal of experimental medicine, 213(1), 35-51.
Transcriptional Control of Dendritic Cell Development.
Murphy Theresa L, Grajales-Reyes Gary E, Wu Xiaodi, Tussiwand Roxane, Briseño Carlos G, Iwata Arifumi, Kretzer Nicole M, Durai Vivek, Murphy Kenneth M (2016), Transcriptional Control of Dendritic Cell Development., in Annual review of immunology, 34, 93-119.
Commensal–dendritic-cell interaction specifies a unique protective skin immune signature
Naik Shruti, Bouladoux Nicolas, Linehan Jonathan L., Han Seong-Ji, Harrison Oliver J., Wilhelm Christoph, Conlan Sean, Himmelfarb Sarah, Byrd Allyson L., Deming Clayton, Quinones Mariam, Brenchley Jason M., Kong Heidi H., Tussiwand Roxanne, Murphy Kenneth M., Merad Miriam, Segre Julia A., Belkaid Yasmine (2015), Commensal–dendritic-cell interaction specifies a unique protective skin immune signature, in Nature, 520(7545), 104-108.
A stromal cell free culture system generates mouse pro-T cells that can reconstitute T-cell compartments in vivo
Gehre Nadine, Nusser Anja, von Muenchow Lilly, Tussiwand Roxane, Engdahl Corinne, Capoferri Giuseppina, Bosco Nabil, Ceredig Rhodri, Rolink Antonius G. (2015), A stromal cell free culture system generates mouse pro-T cells that can reconstitute T-cell compartments in vivo, in EUROPEAN JOURNAL OF IMMUNOLOGY, 45(3), 932-942.
Batf3 maintains autoactivation of Irf8 for commitment of a CD8 alpha(+) conventional DC clonogenic progenitor
Grajales-Reyes Gary E., Iwata Arifumi, Albring Joern, Wu Xiaodi, Tussiwand Roxane, Wumesh K. C., Kretzer Nicole M., Briseno Carlos G., Durai Vivek, Bagadia Prachi, Haldar Malay, Schoenheit Joerg, Rosenbauer Frank, Murphy Theresa L., Murphy Kenneth M. (2015), Batf3 maintains autoactivation of Irf8 for commitment of a CD8 alpha(+) conventional DC clonogenic progenitor, in NATURE IMMUNOLOGY, 16(7), 708-708.
Commensal-dendritic-cell interaction specifies a unique protective skin immune signature
Naik Shruti, Bouladoux Nicolas, Linehan Jonathan L., Han Seong-Ji, Harrison Oliver J., Wilhelm Christoph, Conlan Sean, Himmelfarb Sarah, Byrd Allyson L., Deming Clayton, Quinones Mariam, Brenchley Jason M., Kong Heidi H., Tussiwand Roxanne, Murphy Kenneth M., Merad Miriam, Segre Julia A., Belkaid Yasmine (2015), Commensal-dendritic-cell interaction specifies a unique protective skin immune signature, in NATURE, 520(7545), 104-104.
Klf4 Expression in Conventional Dendritic Cells Is Required for T Helper 2 Cell Responses
Tussiwand Roxane, Everts Bart, Grajales-Reyes Gary E., Kretzer Nicole M., Iwata Arifumi, Bagaitkar Juhi, Wu Xiaodi, Wong Rachel, Anderson David A., Murphy Theresa L., Pearce Edward J., Murphy Kenneth M. (2015), Klf4 Expression in Conventional Dendritic Cells Is Required for T Helper 2 Cell Responses, in IMMUNITY, 42(5), 916-928.
Transcriptional Regulation of Mononuclear Phagocyte Development.
Tussiwand Roxane, Gautier Emmanuel L. (2015), Transcriptional Regulation of Mononuclear Phagocyte Development., in Front Immunology, 6(00533 ), 1-11.
Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging.
Sugiyama N, Sonay AY, Tussiwand R, Cohen BE, Pantazis P, Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging., in Small.

Datasets

Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells

Author Rodrigues, Patrick Fernandes; Alberti-Servera, Llucia; Eremin, Anna; Grajales-Reyes, Gary E.; Ivanek, Robert; Tussiwand, Roxane
Publication date 20.07.2018
Persistent Identifier (PID) https://orcid.org/0000-0002-1712-868X
Repository GEO


Collaboration

Group / person Country
Types of collaboration
Marco Colonna United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
Kenneth Murphy/HHMI Washington University in St. Louis United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Salome Leibundgut, University of Zurich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Immunology intrest group Individual talk When Origin matters 02.11.2018 London, The francis Crick institute, Great Britain and Northern Ireland Tussiwand Roxane;
5th ECI 2018 Individual talk Chair 02.09.2018 Amsterdam, Netherlands Tussiwand Roxane; Eremin Anna;
SOHC_Swiss Experimental Hematology Workshop Talk given at a conference Redefining plasmacytoid dendritic cell development 27.06.2018 Zurich, Switzerland Tussiwand Roxane;
15th International Symposium on Dendritic Cells Talk given at a conference The Origin and True Nature of Plasmacytoid Dendritic Cell 10.06.2018 Aachen, Germany Tussiwand Roxane; Eremin Anna; Fernandes Rodrigues Patrick;
II. European Mononuclear Phagocyte Workshop Talk given at a conference Retreat 04.02.2018 Engelberg, Switzerland Tussiwand Roxane; Fernandes Rodrigues Patrick; Eremin Anna; Simoncini Jacqueline;
“get - together” with the LIMES WiS (Women in Science) group Individual talk Development of Dendritic cells 30.11.2017 Bonn, Germany Tussiwand Roxane;
King' College external Seminar Individual talk Redefining pDC development 23.11.2017 London, Great Britain and Northern Ireland Tussiwand Roxane;
Development of Tissue- and Pathogen-specific Cellular Innate Immunity Basic and Translational Poster The Expression of IRF8 Defines Plasmacytoid Dendritic Cell Commitment 27.09.2017 Freiburg, Germany Eremin Anna; Tussiwand Roxane; Fernandes Rodrigues Patrick;
National Institute of Health site visit Individual talk Dendritic Cell Development 24.07.2017 NIH, Bethesda, United States of America Tussiwand Roxane;
Immunological Symposium Talk given at a conference Dendritic cells: From Development to Function 29.06.2017 Wurzburg, Germany Tussiwand Roxane;
Mini-Symposium: Immunity against Infection and Cancer Talk given at a conference Dendritic Cells: “From Development to Function” 29.06.2017 Wurzburg, Germany Tussiwand Roxane;
Invited speaker at the Centre d'Immunologie de Marseille-Luminy Individual talk Dendritic cells: From Development to Function 27.06.2017 Marseille, France Tussiwand Roxane;
Collaborative Research Center (CRC) 1054, funded by the German Research Foundation (Deutsche Forschungsgemeinschaft - DFG) „Control and Plasticity of Cell-Fate Decisions in the Immune System Individual talk Dendritic cells: From Development to Function 01.06.2017 Munich, Germany Tussiwand Roxane;
TriRhena meeting Talk given at a conference The Expression of IRF8 Defines Plasmacytoid Dendritic Cell Commitment 19.05.2017 Strasbourg, France Tussiwand Roxane;
Keystone Symposia on Mononuclear Phagocytes in Health, Immune Defense and Disease Talk given at a conference The Expression of IRF8 Defines Plasmacytoid Dendritic Cell Commitment 30.04.2017 Austin, United States of America Tussiwand Roxane;
Immunology North East group Individual talk Dendritic Cells from Development to Function 06.04.2017 Newcastle, Great Britain and Northern Ireland Tussiwand Roxane;
interview process for the Earl Stadtman Tenure-Track Investigator Individual talk Dendritic Cells from Development to Function 13.03.2017 NIH, Bethesda, United States of America Tussiwand Roxane;
Experimental and Transplantation Immunology Symposium Talk given at a conference Transcriptional Regulation of Dendritic Cell Biology 29.11.2016 Basel, Switzerland Tussiwand Roxane;
Immunological Diseases and Translation Research Forum 2016 Talk given at a conference Dendritic cells: holding the balance of immunity and tissue integrity 19.10.2016 Shanghai, China Tussiwand Roxane;
The 14th International Symposium on Dendritic Cells (DC 2016) Poster Dendritic cells: holding the balance of immunity and tissue integrity 14.10.2016 Shanghai, China Tussiwand Roxane;
International DC meeting and Dendritic cell workshop Talk given at a conference Dendritic cells: holding the balance of immunity and tissue integrity 14.10.2016 Shanghai, China Fernandes Rodrigues Patrick; Eremin Anna; Tussiwand Roxane;
Leukocyte Biology annual meeting Individual talk Dendritic cells: holding the balance of immunity and tissue integrity 15.09.2016 Verona, Italy Fernandes Rodrigues Patrick; Tussiwand Roxane; Eremin Anna;
DBM Research Day Talk given at a conference Transcriptional Regulation of Dendritic Cell Biology 26.01.2016 Basel, Switzerland Fernandes Rodrigues Patrick; Tussiwand Roxane; Eremin Anna;
4th European Congress of immunology Talk given at a conference Klf4 expression in conventional dendritic cells is required for T helper 2 cell responses 06.09.2015 Vienna, Austria Tussiwand Roxane;
ANNUAL CONGRESS SGAI/SSAI Talk given at a conference Transcriptional Network of DC Development 12.03.2015 Basel, Switzerland Fernandes Rodrigues Patrick; Tussiwand Roxane;
Cell Symposia: the multifaceted Roles of Th2 immunity. Talk given at a conference Klf4 Regulates the development of Th2 priming DCs 10.12.2014 Bruges, Belgium Tussiwand Roxane;


Self-organised

Title Date Place
Next Generation Immunology Cell Symposium 11.02.2018 Tel Aviv, Israel
Fifth Basel Immunology Focus Symposium – BIFS5 28.08.2017 Basel, Switzerland
UBICO Retreat 31.10.2016 Grindelwald, Switzerland
J. Tschopp memorial and annual Swiss immunology society meeting 27.04.2016 Montreux, Switzerland
Ed Palmer retirement symposium 26.03.2016 Basel, Switzerland
European Mononuclear Phagocyte Workshop 06.03.2016 Engelberg, Switzerland
SFB1054 Symposium 02.03.2016 München, Germany
DBM Immunology Retreat 02.11.2015 Grindelwald, Switzerland
DC2014 : 13th International Symposium on Dendritic Cells 14.09.2015 Tours, France
Fourth Basel Immunology Focus Symposium. 31.08.2015 Basel, Switzerland

Awards

Title Year
Poster Prize 2017
G. Jeanette Thorbecke Award (Society for Leukocyte Biology) 2016

Associated projects

Number Title Start Funding scheme
179056 Understanding Dendritic Cell Development and Function 01.10.2018 SNSF Professorships
179056 Understanding Dendritic Cell Development and Function 01.10.2018 SNSF Professorships

Abstract

Dendritic cells (DCs) are professional antigen presenting cells that play a key role in the immune system. Under steady state conditions immature DCs promote tolerance and induction of regulatory cells. Upon pathogen recognition through specific patter recognition receptors (PRR), DCs undergo maturation and initiate the adaptive immune response by priming T cells. DCs dictate the type of adaptive response towards a particular pathogen by secreting different type of cytokines, maintaining an appropriate inflammatory milieu and recruiting specialized effector cells. Such a complex and coordinated response requires DC subset specialization, which is obtained through lineage as well as functional specificity controlled by complex transcriptional networks. It is therefore essential to understand the transcriptional regulation of existing subsets through the characterization of their developmental cues, as well as identify the transcriptional signature required for the functional properties of specific subsets.
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