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Evaluation of environmental impacts in water ecosystems with genetic based bacteriological and physicochemical aspects guided sustainable water resource management in developing countries; Case of south India and sub-Saharan Africa

English title Evaluation of environmental impacts in water ecosystems with genetic based bacteriological and physicochemical aspects guided sustainable water resource management in developing countries; Case of south India and sub-Saharan Africa
Applicant Poté Wembonyama John
Number 150163
Funding scheme Project funding (Div. I-III)
Research institution Institut F.-A. Forel Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Medical Microbiology
Start/End 01.05.2014 - 30.09.2017
Approved amount 293'744.00
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All Disciplines (2)

Discipline
Medical Microbiology
Hydrology, Limnology, Glaciology

Keywords (7)

Lake Geneva, Bay of Vidy; Hospital effluents - latrines - open water ecosyst; soil sediment - heavy metals and organic pollutant; pyrosequencing - antibiotic resistance, ; raw vegetables, - human risks, - water resource; latrine and wells, bacterial diversity, - pathogen; strengthening the capacity - India - Congo DR

Lay Summary (French)

Lead
La résistance aux antibiotiques est une problématique inquiétante pour la santé publique mondiale, d’autant plus exacerbée dans les pays en voie de développement sous climat tropical (comme en Asie du sud et en Afrique sub-saharienne). Plusieurs pays de ces régions ne disposent pas encore de traitements appropriés des effluents provenant des hôpitaux et des eaux usées domestiques et on constate de problèmes épidémiologiques importants. Cette recherche aborde les effets de la dissémination des souches de bactéries pathogènes et de résistance aux antibiotiques, issues des effluents hospitaliers non traités; elle traite de leur propagation dans les eaux de boisson et de baignade ainsi que dans les aliments. Nous allons également procéder à l’évaluation des impacts potentiels sur la santé de la population ainsi exposée.
Lay summary

Les taux élevés de bactéries pathogènes et de bactéries résistantes aux antibiotiques observées dans les écosystèmes aquatiques proviennent essentiellement des effluents des stations d’épurations des eaux domestiques et des rejets des eaux hospitalières non ou partiellement traitées. Dans plusieurs régions du monde, ces effluents sont rejetés dans l’environnement sans traitement préalable. Dans la plupart des pays en voie de développement, les eaux de rivières et des nappes recevant ces effluents sont souvent utilisées comme eaux de consommation, de baignade et pour l’irrigation des cultures maraîchères. La situation est plus préoccupante dans les grandes villes des pays d’Asie du sud, de l’Amérique du sud et de l’Afrique sub-saharienne. De plus en plus de problèmes épidémiologiques ainsi qu’une forte mortalité infantile liés à l’utilisation de ces eaux y sont observées.  Cette recherche poursuit les objectifs suivants:

  • Evaluation de la dissémination et de persistance des bactéries pathogènes et des gènes de résistances aux antibiotiques dans les rivières et les puits recevant les effluents hospitaliers non traitées, sous climat tropical
  • Analyses bactériologues des produits de consommation issus des cultures utilisant ces eaux pour l’irrigation
  • Evaluation des risques pour la santé de la population et mise en place de possibilités de remédiation.
Les méthodes et les scénarios qui seront développés comprendront entre autres les stratégies pour le développement de la nouvelle pratique de gestion de l'eau et de la production alimentaire durable, ainsi que de la prévention des risques sur la santé humaine. Ce projet propose aussi le renforcement de capacités via des enseignements aux chercheurs locaux et des modalités de transfert de technologies appropriées à cette problématique. L’étude sera réalisée dans certains sites au sud de l’Inde, dans la province de Tamil-Nadou, en République Démocratique du Congo et dans le site de Vidy, Lac Léman (Lausanne, Suisse).
Direct link to Lay Summary Last update: 03.02.2014

Responsible applicant and co-applicants

Employees

Name Institute

Publications

Publication
Accumulation of toxic metals and organic micro-pollutants in sediments from tropical urban rivers, Kinshasa, Democratic Republic of the Congo
Kilunga Pitchouna I., Sivalingam Periyasamy, Laffite Amandine, Grandjean Dominique, Mulaji Crispin K., de Alencastro Luiz Felippe, Mpiana Pius T., Poté John (2017), Accumulation of toxic metals and organic micro-pollutants in sediments from tropical urban rivers, Kinshasa, Democratic Republic of the Congo, in Chemosphere, 179, 37-48.
Antibiotic resistant Pseudomonas spp. in the aquatic environment: A prevalence study under tropical and temperate climate conditions
Devarajan Naresh, Köhler Thilo, Sivalingam Periyasamy, van Delden Christian, Mulaji Crispin K., Mpiana Pius T., Ibelings Bastiaan W., Poté John (2017), Antibiotic resistant Pseudomonas spp. in the aquatic environment: A prevalence study under tropical and temperate climate conditions, in Water Research, 115, 256-265.
Microbiological quality of water in a city with persistent and recurrent waterborne diseases under tropical sub-rural conditions: The case of Kikwit City, Democratic Republic of the Congo
Nienie Alexis B., Periyasamy Sivalingam, Laffite Amandine, Ngelinkoto Patience, Otamonga Jean-Paul, Matand Alphonse, Mulaji Crispin K., Biey Emmanuel M., Mpiana Pius T., Pote-Wembonyama John (2017), Microbiological quality of water in a city with persistent and recurrent waterborne diseases under tropical sub-rural conditions: The case of Kikwit City, Democratic Republic of the Congo, in International Journal of Hygiene and Environmental Health, 220(5), 820-828.
Seasonal variability of water quality by physicochemical indexes and traceable metals in suburban area in Kikwit, Democratic Republic of the Congo
Nienie Alexis B., Periyasamy Sivalingam, Laffite Amandine, Ngelinkoto Patience, Otamonga Jean-Paul, Matand Alphonse, Mulaji Crispin K., Mubedi Josué I., Mpiana Pius T., Pote-Wembonyama John (2017), Seasonal variability of water quality by physicochemical indexes and traceable metals in suburban area in Kikwit, Democratic Republic of the Congo, in International Soil and Water Conservation Research, 5(2), 158-165.
Evaluation of Water Quality from Suburban Shallow Wells Under Tropical Conditions According to the Seasonal Variation, Bumbu, Kinshasa, Democratic Republic of the Congo
Kapembo Michel L., Laffite Amandine, Bokolo Mathieu K., Mbanga Aimé L., Maya-Vangua Marc M., Otamonga Jean-Paul, Mulaji Crispin K., Mpiana Pius T., Wildi Walter, Poté John (2016), Evaluation of Water Quality from Suburban Shallow Wells Under Tropical Conditions According to the Seasonal Variation, Bumbu, Kinshasa, Democratic Republic of the Congo, in Exposure and Health, 8(4), 487-496.
Hospital Effluents Are One of Several Sources of Metal, Antibiotic Resistance Genes, and Bacterial Markers Disseminated in Sub-Saharan Urban Rivers
Laffite Amandine, Kilunga Pitchouna I., Kayembe John M., Devarajan Naresh, Mulaji Crispin K., Giuliani Gregory, Slaveykova Vera I., Poté John (2016), Hospital Effluents Are One of Several Sources of Metal, Antibiotic Resistance Genes, and Bacterial Markers Disseminated in Sub-Saharan Urban Rivers, in Frontiers in Microbiology, 7, 1128.
The impact of hospital and urban wastewaters on the bacteriological contamination of the water resources in Kinshasa, Democratic Republic of Congo
Kilunga Pitchouna I., Kayembe John M., Laffite Amandine, Thevenon Florian, Devarajan Naresh, Mulaji Crispin K., Mubedi Josué I., Yav Zéphirin G., Otamonga Jean-Paul, Mpiana Pius T., Poté John (2016), The impact of hospital and urban wastewaters on the bacteriological contamination of the water resources in Kinshasa, Democratic Republic of Congo, in Journal of Environmental Science and Health, Part A, 51(12), 1034-1042.
Occurrence of Antibiotic Resistance Genes and Bacterial Markers in a Tropical River Receiving Hospital and Urban Wastewaters
Devarajan Naresh, Laffite Amandine, Mulaji Crispin Kyela, Otamonga Jean-Paul, Mpiana Pius Tshimankinda, Mubedi Josué Ilunga, Prabakar Kandasamy, Ibelings Bastiaan Willem, Poté John (2016), Occurrence of Antibiotic Resistance Genes and Bacterial Markers in a Tropical River Receiving Hospital and Urban Wastewaters, in PLOS ONE, 11(2), e0149211-e0149211.
Hospital and urban effluent waters as a source of accumulation of toxic metals in the sediment receiving system of the Cauvery River, Tiruchirappalli, Tamil Nadu, India
Devarajan Naresh, Laffite Amandine, Ngelikoto Patience, Elongo Vicky, Prabakar Kandasamy, Mubedi Josué I., Piana Pius T. M., Wildi Walter, Poté John (2015), Hospital and urban effluent waters as a source of accumulation of toxic metals in the sediment receiving system of the Cauvery River, Tiruchirappalli, Tamil Nadu, India, in Environmental Science and Pollution Research, 22(17), 12941-12950.
Accumulation of Clinically Relevant Antibiotic-Resistance Genes, Bacterial Load, and Metals in Freshwater Lake Sediments in Central Europe
Devarajan Naresh, Laffite Amandine, Graham Neil D., Meijer Maria, Prabakar Kandasamy, Mubedi Josué I., Elongo Vicky, Mpiana Pius T., Ibelings Bastiaan Willem, Wildi Walter, Poté John (2015), Accumulation of Clinically Relevant Antibiotic-Resistance Genes, Bacterial Load, and Metals in Freshwater Lake Sediments in Central Europe, in Environmental Science & Technology, 49(11), 6528-6537.
Trace metal distributions in the sediments from river-reservoir systems: case of the Congo River and Lake Ma Vallée, Kinshasa (Democratic Republic of Congo)
Mwanamoki Paola M., Devarajan Naresh, Niane Birane, Ngelinkoto Patience, Thevenon Florian, Nlandu José W., Mpiana Pius T., Prabakar Kandasamy, Mubedi Josué I., Kabele Christophe G., Wildi Walter, Poté John (2015), Trace metal distributions in the sediments from river-reservoir systems: case of the Congo River and Lake Ma Vallée, Kinshasa (Democratic Republic of Congo), in Environmental Science and Pollution Research, 22(1), 586-597.
Assessment of pathogenic bacteria in water and sediment from a water reservoir under tropical conditions (Lake Ma Vallée), Kinshasa Democratic Republic of Congo
Mwanamoki Paola M., Devarajan Naresh, Thevenon Florian, Atibu Emmanuel K., Tshibanda Joseph B., Ngelinkoto Patience, Mpiana Pius T., Prabakar Kandasamy, Mubedi Josué I., Kabele Christophe G., Wildi Walter, Poté John (2014), Assessment of pathogenic bacteria in water and sediment from a water reservoir under tropical conditions (Lake Ma Vallée), Kinshasa Democratic Republic of Congo, in Environmental Monitoring and Assessment, 186(10), 6821-6830.
Microbiological and physicochemical characterization of water and sediment of an urban river: N’Djili River, Kinshasa, Democratic Republic of the Congo
Tshibanda Joseph B., Devarajan Naresh, Birane Niane, Mwanamoki Paola M., Atibu Emmanuel K., Mpiana Pius T., Prabakar Kandasamy, Mubedi Ilunga Josué, Wildi Walter, Poté John (2014), Microbiological and physicochemical characterization of water and sediment of an urban river: N’Djili River, Kinshasa, Democratic Republic of the Congo, in Sustainability of Water Quality and Ecology, 3-4, 47-54.
Trace metals and persistent organic pollutants in sediments from river-reservoir systems in Democratic Republic of Congo (DRC): Spatial distribution and potential ecotoxicological effects
Mwanamoki Paola M., Devarajan Naresh, Thevenon Florian, Birane Niane, de Alencastro Luiz Felippe, Grandjean Dominique, Mpiana Pius T., Prabakar Kandasamy, Mubedi Josué I., Kabele Christophe G., Wildi Walter, Poté John (2014), Trace metals and persistent organic pollutants in sediments from river-reservoir systems in Democratic Republic of Congo (DRC): Spatial distribution and potential ecotoxicological effects, in Chemosphere, 111, 485-492.

Collaboration

Group / person Country
Types of collaboration
Profs. Vera Slaveykova and Walter Wildi, Forel department Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Kandasamy Prabakar/Bharathidasan University India (Asia)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Prof. Josué Mubedi Ilunga/National Pedagogical University of Congo Congo Democratic Republic (formerly Zaire) (Africa)
- Publication
- Research Infrastructure
- Exchange of personnel
PD, Dr. TONOLLA, Mauro Amedeo, Istituto Cantonale di Microbiologia Switzerland (Europe)
- Research Infrastructure
Prof. MPIANA TSHIMANKINDA/University of Kinshasa Congo Democratic Republic (formerly Zaire) (Africa)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Seminar on the Problematic of antibiotic resistance Talk given at a conference Dissemination of Antibiotic Resistance Bacteria and their Resistance Genes in non-clinical Environments 14.10.2016 Global health Institute (Genève), Switzerland Poté Wembonyama John; Laffite Amandine;
Exploring research in aquatic environment: Interdisciplinary research performed in collaboration between University of Geneva, University of Kinshasa and National Pedagogic University Talk given at a conference Dissemination of antibiotic resistance genes in tropical rivers subjected to different urban waters, Kinshasa, RD Congo 18.06.2016 Kinshasa, Congo Democratic Republic (formerly Zaire) Laffite Amandine; Poté Wembonyama John; Wildi Walter;
SME 2015 – 6th Swiss Microbial Ecology Meeting Poster Effect of hospital effluents discharge on accumulation of toxic metals and antibiotic resistance genes in aquatic sub-saharian sediments 10.09.2015 Asconà, Switzerland Laffite Amandine;


Self-organised

Title Date Place
Prevalence of antibiotic resistance genes in clinical and environmental populations 20.09.2017 University of Geneva, Switzerland
La communication environnementale dans les pays en voie de développement 27.06.2014 Kinshasa , Congo Democratic Republic (formerly Zaire)

Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Formation sur la Gestion Intégrée des Ressources en Eau en Afrique : Pour une gouvernance de l’eau qui intègre les Objectifs de Développement Durable et les droits humains à l’eau et à l’assainissement Talk 01.12.2016 Waterlex OMM (Genève), Switzerland Poté Wembonyama John;
Dissemination of antibiotic resistance bacteria and their resistance genes in non-clinical environment Talk 14.10.2016 Genève, Switzerland Poté Wembonyama John; Laffite Amandine;
Exploring research in aquatic environment: Interdisciplinary research performed in collaboration between University of Geneva, University of Kinshasa and National Pedagogic University Talk 18.06.2016 Kinshasa, Congo Democratic Republic (formerly Zaire) Poté Wembonyama John; Laffite Amandine; Wildi Walter;
Groupe de travail antibioresistance Talk 14.03.2016 Genève, Switzerland Poté Wembonyama John; Laffite Amandine;


Communication with the public

Communication Title Media Place Year
Media relations: print media, online media Les bactéries font de la résistance Journal “Lémaniques” 103/3-2017 Western Switzerland 2017
Media relations: print media, online media A travers un grand séminaire : UNIGE-UPN-UNIKIN se coalisent pour la sensibilisation sur la pollutio Sangoya Congo International 2016
Media relations: radio, television Antibiotiques pour animaux : on en paie le prix 36.9 RTS Western Switzerland 2016
Media relations: print media, online media Dans les eaux troubles du Congo Journal universitaire Campus n°122 Western Switzerland 2016
New media (web, blogs, podcasts, news feeds etc.) Ecosystem Interface: Interdisciplinary Overview MOOCs International 2016
Media relations: print media, online media La coopération Suisse en action. RDC : l’UNIKIN et l’UPN bénéficient de laboratoires Hi-Tech 7sur7 International 2016

Awards

Title Year
Young Scientist Awards for the Best Poster Presentation during the 27th SETAC Europe Annual Meeting in Brussels, Belgium 2017

Associated projects

Number Title Start Funding scheme
173281 Evaluation of environmental impacts in water ecosystems with genetic based bacteriological and physicochemical aspects guided sustainable water resource management in developing countries; Case of south India and sub-Saharan Africa 01.10.2017 Project funding (Div. I-III)

Abstract

Building and strengthening of capacity in developing countries can be considered as one of majors aspects of sustainable development in the World. The challenging water contamination, natural resources management as well as food production have the global consequences. For this proposal, the approaches are chosen to identify the most critical problems related to water contamination in general, particularly in developing countries such as India and Congo DR. The objective of this present study is to study the effect of hospital effluents on the distribution and prevalence of antibiotic resistant bacteria and their resistance genes from the surface waters and sediments of receiving systems under tropical conditions and the impact of population and latrines on fecal contamination in ponds and wells (both receiving system) will be assessed. Both aspects are highly relevant from a global perspective for the economic, social and environmental development throughout the world. This project will also examine the environmental exposure pathways particularly anthropogenic influences associated with aquatic environment receiving systems, irrigation water and crops, and drinking water supplies. Different hypotheses and scenarios will be considered in this project:•How do hospital effluents affect the quality of water in south India river, Cauvery basin with respect to the prevalence of Antibiotic Resistance Genes (ARGs) in bacterial population of surface and sediments?•How the river ecosystem contaminated with hospital effluents for multidrug resistant bacteria with antibiotic resistance genes act as potential reservoir?•To assess the transfer ability of ARGs to environmental commensal bacteria through gene transfer mechanism.•Impacts of environmental factors on the persistence of pathogens under tropical conditions; •Assessment of pathogens and adenovirus related to the number of latrines around the pond ecosystem, as well as the types of latrine with respect to prevalence of antibiotic resistant bacteria associated with local population density receiving water.•Impact of population and latrines on pathogens contamination of ponds and wells: prevalence of ARGs associated pathogens, human risk assessment and environmental pathways. •Understanding the potential risk associated with raw vegetables and to establish whether irrigation contaminated water is a possible source of pathogens (such as Enterococcus, E. coli, E. coli somatic, Clostridium spores, Salmonella paratyphi A, S. typhii, and Vibrio cholerea) in fresh produce. The novelty and originality of this project reside in a combination of socio-cultural aspects and other aspects which cause epidemics and environmental impacts of contaminated aquatic ecosystem. The methods and scenarios which will be developed include the potential strategy for the development of new sustainable water management and food production as well as to prevent the human risks in Asia and Sub-Sahara Africa. The key innovation of the project and methodologies applied is the combination of water quality variation frequency and the impact magnitude function to quantify the risk related with water quality variation in developing countries under tropical condition. The results of the present project will be disseminated and shared to the public with the hope to catalyze the consciousness and responsibilities of the public opinion to understand the importance and interrelation between human and environment impacts with regard to water management.
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