chronic infection; bacterial pathogenesis; immune response; type IV secretion ; bacterial effector protein; Bartonella; yeast; Brucella; animal infection model
Andritschke Daniel, Dilling Sabrina, Emmenlauer Mario, Welz Tobias, Schmich Fabian, Misselwitz Benjamin, Rämö Pauli, Rottner Klemens, Kerkhoff Eugen, Wada Teiji, Penninger Josef M, Beerenwinkel Niko, Horvath Peter, Dehio Christoph, Hardt Wolf-Dietrich (2016), A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Invasion., in PloS one
, 11(9), 0161965-0161965.
Harms Alexander, Stanger Frédéric V, Dehio Christoph (2016), Biological Diversity and Molecular Plasticity of FIC Domain Proteins., in Annual review of microbiology
, 70, 341-60.
Stanger Frédéric V, Harms Alexander, Dehio Christoph, Schirmer Tilman (2016), Crystal Structure of the Escherichia coli Fic Toxin-Like Protein in Complex with Its Cognate Antitoxin., in PloS one
, 11(9), 0163654-0163654.
Stanger Frédéric V, Burmann Björn M, Harms Alexander, Aragão Hugo, Mazur Adam, Sharpe Timothy, Dehio Christoph, Hiller Sebastian, Schirmer Tilman (2016), Intrinsic regulation of FIC-domain AMP-transferases by oligomerization and automodification., in Proceedings of the National Academy of Sciences of the United States of America
, 113(5), 529-37.
Casanova Alain, Low Shyan H, Emmenlauer Mario, Conde-Alvarez Raquel, Salcedo Suzana P, Gorvel Jean-Pierre, Dehio Christoph (2016), Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells., in Journal of visualized experiments : JoVE
, (114), 1.
Harms Alexander, Stanger Frédéric Valentin, Scheu Patrick Daniel, de Jong Imke Greet, Goepfert Arnaud, Glatter Timo, Gerdes Kenn, Schirmer Tilman, Dehio Christoph (2015), Adenylylation of Gyrase and Topo IV by FicT Toxins Disrupts Bacterial DNA Topology., in Cell reports
, 12(9), 1497-507.
Kreibich Saskia, Emmenlauer Mario, Fredlund Jennifer, Rämö Pauli, Münz Christian, Dehio Christoph, Enninga Jost, Hardt Wolf-Dietrich (2015), Autophagy Proteins Promote Repair of Endosomal Membranes Damaged by the Salmonella Type Three Secretion System 1., in Cell host & microbe
, 18(5), 527-37.
Kühbacher Andreas, Emmenlauer Mario, Rämo Pauli, Kafai Natasha, Dehio Christoph, Cossart Pascale, Pizarro-Cerdá Javier (2015), Genome-Wide siRNA Screen Identifies Complementary Signaling Pathways Involved in Listeria Infection and Reveals Different Actin Nucleation Mechanisms during Listeria Cell Invasion and Actin Comet Tail Formation., in mBio
, 6(3), 00598-15.
Schmich Fabian, Szczurek Ewa, Kreibich Saskia, Dilling Sabrina, Andritschke Daniel, Casanova Alain, Low Shyan Huey, Eicher Simone, Muntwiler Simone, Emmenlauer Mario, Rämö Pauli, Conde-Alvarez Raquel, von Mering Christian, Hardt Wolf-Dietrich, Dehio Christoph, Beerenwinkel Niko (2015), gespeR: a statistical model for deconvoluting off-target-confounded RNA interference screens., in Genome biology
, 16, 220-220.
Siebourg-Polster Juliane, Mudrak Daria, Emmenlauer Mario, Rämö Pauli, Dehio Christoph, Greber Urs, Fröhlich Holger, Beerenwinkel Niko (2015), NEMix: single-cell nested effects models for probabilistic pathway stimulation., in PLoS computational biology
, 11(4), 1004078-1004078.
Siamer Sabrina, Dehio Christoph (2015), New insights into the role of Bartonella effector proteins in pathogenesis., in Current opinion in microbiology
, 23, 80-5.
Okujava Rusudan, Guye Patrick, Lu Yun-Yueh, Mistl Claudia, Polus Florine, Vayssier-Taussat Muriel, Halin Cornelia, Rolink Antonius G, Dehio Christoph (2014), A translocated effector required for Bartonella dissemination from derma to blood safeguards migratory host cells from damage by co-translocated effectors., in PLoS pathogens
, 10(6), 1004187-1004187.
Pieles Kathrin, Glatter Timo, Harms Alexander, Schmidt Alexander, Dehio Christoph (2014), An experimental strategy for the identification of AMPylation targets from complex protein samples., in Proteomics
, 14(9), 1048-52.
Stekhoven Daniel J, Omasits Ulrich, Quebatte Maxime, Dehio Christoph, Ahrens Christian H (2014), Proteome-wide identification of predominant subcellular protein localizations in a bacterial model organism., in Journal of proteomics
, 99, 123-37.
Rämö Pauli, Drewek Anna, Arrieumerlou Cécile, Beerenwinkel Niko, Ben-Tekaya Houchaima, Cardel Bettina, Casanova Alain, Conde-Alvarez Raquel, Cossart Pascale, Csúcs Gábor, Eicher Simone, Emmenlauer Mario, Greber Urs, Hardt Wolf-Dietrich, Helenius Ari, Kasper Christoph, Kaufmann Andreas, Kreibich Saskia, Kühbacher Andreas, Kunszt Peter, Low Shyan Huey, Mercer Jason, Mudrak Daria, Muntwiler Simone, Pelkmans Lucas (2014), Simultaneous analysis of large-scale RNAi screens for pathogen entry., in BMC genomics
, 15, 1162-1162.
Franceschini Andrea, Meier Roger, Casanova Alain, Kreibich Saskia, Daga Neha, Andritschke Daniel, Dilling Sabrina, Rämö Pauli, Emmenlauer Mario, Kaufmann Andreas, Conde-Álvarez Raquel, Low Shyan Huey, Pelkmans Lucas, Helenius Ari, Hardt Wolf-Dietrich, Dehio Christoph, von Mering Christian (2014), Specific inhibition of diverse pathogens in human cells by synthetic microRNA-like oligonucleotides inferred from RNAi screens., in Proceedings of the National Academy of Sciences of the United States of America
, 111(12), 4548-53.
Stanger Frédéric V, Dehio Christoph, Schirmer Tilman (2014), Structure of the N-terminal Gyrase B fragment in complex with ADP⋅Pi reveals rigid-body motion induced by ATP hydrolysis., in PloS one
, 9(9), 107289-107289.
Alperi Anabel, Larrea Delfina, Fernández-González Esther, Dehio Christoph, Zechner Ellen L, Llosa Matxalen (2013), A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative type IV secretion system., in Journal of bacteriology
, 195(22), 4999-5006.
Omasits Ulrich, Quebatte Maxime, Stekhoven Daniel J, Fortes Claudia, Roschitzki Bernd, Robinson Mark D, Dehio Christoph, Ahrens Christian H (2013), Directed shotgun proteomics guided by saturated RNA-seq identifies a complete expressed prokaryotic proteome., in Genome research
, 23(11), 1916-27.
Québatte Maxime, Dick Mathias S, Kaever Volkhard, Schmidt Alexander, Dehio Christoph (2013), Dual input control: activation of the Bartonella henselae VirB/D4 type IV secretion system by the stringent sigma factor RpoH1 and the BatR/BatS two-component system., in Molecular microbiology
, 90(4), 756-75.
Kühbacher Andreas, Gouin Edith, Mercer Jason, Emmenlauer Mario, Dehio Christoph, Cossart Pascale, Pizarro-Cerdá Javier (2013), Imaging InlC secretion to investigate cellular infection by the bacterial pathogen Listeria monocytogenes., in Journal of visualized experiments : JoVE
, (79), 51043-51043.
Farhan Muhammad, Ruusuvuori Pekka, Emmenlauer Mario, Rämö Pauli, Dehio Christoph, Yli-Harja Olli (2013), Multi-scale Gaussian representation and outline-learning based cell image segmentation., in BMC bioinformatics
, 14 Suppl 10, 6-6.
Stanger Frédéric V, de Beer Tjaart A P, Dranow David M, Schirmer Tilman, Phan Isabelle, Dehio Christoph, The BID Domain of Type IV Secretion Substrates Forms a Conserved Four-Helix Bundle Topped with a Hook., in Structure (London, England : 1993)
In the initial funding period of the SNSF grant 31003A-132979 we used Bartonella and Brucella - two related zoonotic pathogens engaging the widespread type IV secretion (T4S) mechanism for establishing chronic bacterial infection - as models to study the cellular, molecular and evolutionary basis of the subversion of host cell functions by T4S-translocated effector proteins. For the three year prolongation period the established multidisciplinary experimental approach will be extended by adopting yeast as surrogate model for studying conserved eukaryotic processes targeted by T4S effectors. Subproject A will focus on the structure/function analysis of Bartonella effector proteins (Beps) translocated by the VirB T4S system of Bartonella and their physiological consequences on the host, with particular emphasis on studying the subversion of immune signaling processes. Subproject B will focus on the functional analysis of effector proteins translocated by the distinct VirB T4S system of Brucella and their physiological consequences on host cell interaction, with particular emphasis on studying the intracellular trafficking events from a late endosomal compartment, where the VirB system is activated in response to acidification, to the endoplasmic reticulum (ER)-related replicative niche of Brucella. Together, these studies will contribute to establishing a molecular paradigm for the role of T4S effectors in triggering chronic bacterial infection.