Heart; Liver; Skeletal muscle; Insulin resistance; Magnetic resonance imaging; Magnetic resonance spectroscopy
Rosset Robin, Lecoultre Virgile, Egli Léonie, Cros Jérémy, Dokumaci Ayse Sila, Zwygart Karin, Boesch Chris, Kreis Roland, Schneiter Philippe, Tappy Luc (2017), Postexercise repletion of muscle energy stores with fructose or glucose in mixed meals., in The American journal of clinical nutrition
, [ePub ahead], N/A.
Christ Emanuel R, Egger Andrea, Allemann Sabin, Buehler Tania, Kreis Roland, Boesch Chris (2016), Effects of aerobic exercise on ectopic lipids in patients with growth hormone deficiency before and after growth hormone replacement therapy., in Scientific reports
, 6, 19310-19310.
Valenzuela Waldo, Ferguson Stephen J, Ignasiak Dominika, Diserens Gaëlle, Häni Levin, Wiest Roland, Vermathen Peter, Boesch Chris, Reyes Mauricio (2016), FISICO: Fast Image SegmentatIon COrrection., in PloS one
, 11(5), 0156035-0156035.
Boesch Chris, Kreis Roland (2016), Handbook of Magnetic Resonance Spectroscopy In Vivo, Chapter Muscle Studies by H-1 MRS, in Griffiths John and Bottomley Paul (ed.), John Wiley & Sons, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA , 1097-1108.
MacMillan Erin Leigh, Bolliger Christine Sandra, Boesch Chris, Kreis Roland (2016), Influence of muscle fiber orientation on water and metabolite relaxation times, magnetization transfer, and visibility in human skeletal muscle., in Magnetic resonance in medicine
, 75(4), 1764-70.
Bally Lia, Zueger Thomas, Buehler Tania, Dokumaci Ayse S, Speck Christian, Pasi Nicola, Ciller Carlos, Paganini Daniela, Feller Katrin, Loher Hannah, Rosset Robin, Wilhelm Matthias, Tappy Luc, Boesch Chris, Stettler Christoph (2016), Metabolic and hormonal response to intermittent high-intensity and continuous moderate intensity exercise in individuals with type 1 diabetes: a randomised crossover study., in Diabetologia
, 59(4), 776-84.
Buehler Tania, Bally Lia, Dokumaci Ayse Sila, Stettler Christoph, Boesch Chris (2016), Methodological and physiological test-retest reliability of (13) C-MRS glycogen measurements in liver and in skeletal muscle of patients with type 1 diabetes and matched healthy controls., in NMR in biomedicine
, 29(6), 796-805.
Dokumaci Ayse Sila, Pouymayou Bertrand, Kreis Roland, Boesch Chris (2016), Motion-insensitive determination of B1+ amplitudes based on the bloch-siegert shift in single voxels of moving organs including the human heart., in Magnetic resonance in medicine
, 75(5), 1867-74.
Zueger Thomas, Loher Hannah, Egger Andrea, Boesch Chris, Christ Emanuel (2016), Regulation of fuel metabolism during exercise in hypopituitarism with growth hormone-deficiency (GHD)., in Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the Inte
, 29, 39-44.
Pouymayou Bertrand, Buehler Tania, Kreis Roland, Boesch Chris (2016), Test-retest analysis of multiple (31) P magnetization exchange pathways using asymmetric adiabatic inversion., in Magnetic resonance in medicine
, [ePub ahead], N/A.
Loher Hannah, Kreis Roland, Boesch Chris, Christ Emanuel (2016), The Flexibility of Ectopic Lipids., in International journal of molecular sciences
, 17(9), E1554.
Buehler Tania, Kreis Roland, Boesch Chris (2015), Comparison of (31)P saturation and inversion magnetization transfer in human liver and skeletal muscle using a clinical MR system and surface coils., in NMR in biomedicine
, 28(2), 188-99.
Valenzuela Waldo, Ferguson Stephen J, Ignasiak Dominika, Diserens Gaelle, Vermathen Peter, Boesch Chris, Reyes Mauricio (2015), Correction tool for Active Shape Model based lumbar muscle segmentation., in Conf Proc IEEE Eng Med Biol Soc.
, IEEE, New York, NY 10016-5997 USA.
Broskey Nicholas T, Boss Andreas, Fares Elie-Jacques, Greggio Chiara, Gremion Gerald, Schlüter Leo, Hans Didier, Kreis Roland, Boesch Chris, Amati Francesca (2015), Exercise efficiency relates with mitochondrial content and function in older adults., in Physiological reports
, 3(6), e12418.
Bally L, Buehler T, Dokumaci A S, Boesch C, Stettler C (2015), Hepatic and intramyocellular glycogen stores in adults with type 1 diabetes and healthy controls., in Diabetes research and clinical practice
, 109(1), 1-3.
Lee SoJung, Rivera-Vega Michelle, Alsayed Hany Mohamed Abdel Aal, Boesch Chris, Libman Ingrid (2015), Metabolic inflexibility and insulin resistance in obese adolescents with non-alcoholic fatty liver disease., in Pediatric diabetes
, 16(3), 211-8.
Brandejsky Vaclav, Boesch Chris, Kreis Roland (2015), Proton diffusion tensor spectroscopy of metabolites in human muscle in vivo., in Magnetic resonance in medicine
, 73(2), 481-7.
Campos Vanessa, Despland Camille, Brandejsky Vaclav, Kreis Roland, Schneiter Philippe, Chiolero Arnaud, Boesch Chris, Tappy Luc (2015), Sugar- and artificially sweetened beverages and intrahepatic fat: A randomized controlled trial., in Obesity (Silver Spring, Md.)
, 23(12), 2335-9.
Ith Michael, Stettler Christoph, Xu Jian, Boesch Chris, Kreis Roland (2014), Cardiac lipid levels show diurnal changes and long-term variations in healthy human subjects., in NMR in biomedicine
, 27(11), 1285-92.
Broskey Nicholas T, Greggio Chiara, Boss Andreas, Boutant Marie, Dwyer Andrew, Schlueter Leopold, Hans Didier, Gremion Gerald, Kreis Roland, Boesch Chris, Canto Carles, Amati Francesca (2014), Skeletal muscle mitochondria in the elderly: effects of physical fitness and exercise training., in The Journal of clinical endocrinology and metabolism
, 99(5), 1852-61.
Background:Insulin resistance (IR) is a common denominator of a complex of endemic diseases. This includes obesity, the “metabolic syndrome”, and overt Type 2 diabetes mellitus, all with severe cardiovascular consequences. The related non-alcoholic fatty liver disease (NAFLD) can lead to steatohepatitis (NASH) and subsequently to hepatocellular carcinoma. Lipotoxicity, mitochondrial impairment, inflammation, and oxidative stress are possible culprits leading to IR. Since most of these mechanisms are related to the complex of lipid droplets and mitochondria, these organelles are increasingly studied. In particular lipid organelles are organized to a higher degree and perform more complex functions than assumed previously.Magnetic resonance spectroscopy (MRS) is a non-invasive, non-ionizing tool for in vivo studies in humans and animals that is related to high-resolution nuclear magnetic resonance (HR-NMR) and magic angle spinning (HR-MAS). It can provide information on the complex of lipid droplets and mitochondria and is thus an attractive tool to validate hypotheses in vivo that have been generated in well-defined systems of extracts, cells, and excised tissue.Hypotheses:This proposal includes three sub-projects:1) Characterization of lipid organelles (lipid composition).2) ATP synthase flux measured by 31P-MRS saturation transfer.3) Multinuclear MRS of liver diseases (NAFLD, NASH).We hypothesize that in vivo MRS is sensitive enough to detect:A) Changes of composition of ectopic lipids in muscle, liver, and heart with exercise, diet, and under influence of IR.B) Influences of diet, exercise, and IR on the flux of ATP synthase measured by 31P-MRS saturation transfer.C) Differences between NAFLD and NASH in vivo using multinuclear MRS.Methodology:A feasibility study has shown that in vivo MRS is able to follow changes of liver metabolism (lipid content, glycogen, high-energy phosphates, and ATP synthase flux) in a cohort of healthy subjects. However, it needs further development to overcome the inherently low signal-to-noise-ratio and to distinguish the distinct lipid resonances to determine lipid composition. In addition, in vivo single-voxel MRS will be complemented with 2-(spectral)-dimensional MRS and it will be correlated with HR-MAS of excised tissue from muscle and (clinically indicated) liver biopsies. In a proof-of-principle with small cohorts, effects of exercise, diet, and IR will be shown.Importance and impact:Our network of MR-group, physiologists, and clinicians is working continuously on the development and - equally important - the application of in vivo MRS methods. The truly translational nature of HR-NMR, HR-MAS, and in vivo MRS in animals and humans allows for a stepwise increment of complexity of the examined system. This grant proposal will combine and foster the combination of HR-MAS in excised yet intact tissue with in vivo MRS in human subjects. This will have impact on the understanding of IR and will allow for a validation of hypotheses from well-defined systems in the complex environment of the human body.