Membrane biosynthesis; Orientation de site actif; Analyse structurelle; Acyltransférase; Dérivatisation chimique; Spectrometrie de masse; Marquage isotopique
Debelyy Mykhaylo O., Waridel Patrice, Quadroni Manfredo, Schneiter Roger, Conzelmann Andreas (2017), Chemical crosslinking and mass spectrometry to elucidate the topology of integral membrane proteins, in PLOS ONE
, 12(10), e0186840-e0186840.
Vazquez Hector M (2016), Chemogenetic E-MAP in Saccharomyces cerevisiae for Identification of Membrane Transporters Operating Lipid Flip Flop., in PLoS Genet.
Mallela kumar Shamroop (2016), Functions of Ceramide Synthase Paralogs YPR114w and YJR116w of Saccharomyces cerevisiae., in PLoS One
Rovillos MJ (2016), Structural characterization of suppressor lipids by high-resolution mass spectrometry, in Rapid Commun Mass Spectrom
Bavdek Andrej (2015), Enzyme-coupled assays for flip-flop of acyl-Coenzyme A in liposomes., in Biochim Biophys Acta
Voynova Natalia S (2015), Saccharomyces cerevisiae Is Dependent on Vesicular Traffic between the Golgi Apparatus and the Vacuole When Inositolphosphorylceramide Synthase Aur1 Is Inactivated., in Eukaryot Cell
Bochud Arlette (2015), The active site of yeast phosphatidylinositol synthase Pis1 is facing the cytosol., in Biochim Biophys Acta
Vazquez Hector M (2014), Cdc1 removes the ethanolamine phosphate of the first mannose of GPI anchors and thereby facilitates the integration of GPI proteins into the yeast cell wall., in Mol Biol Cell
Voynova Natalia S (2014), Characterization of yeast mutants lacking alkaline ceramidases YPC1 and YDC1., in FEMS Yeast Res.
Bochud Arlette, Ramachandra Nagaraju, Conzelmann Andreas (2013), Adaptation of low-resolution methods for the study of yeast microsomal polytopic membrane proteins: a methodological review., in Biochem Soc Trans.
Ramachandra Nagaraju, Conzelmann Andreas (2013), Membrane topology of yeast alkaline ceramidase YPC1., in Biochem J.
The proposed experiments aim at a better understanding of the regulation of cell growth and ER maintenance through lipid biosynthetic enzymes. The Endoplasmic reticulum (ER) is a very large intracellular organelle devoted mainly to the biosynthesis of membrane and soluble proteins as well as of lipids for the secretory organelles and the extracellular compartment. A major effort will be made to locate the catalytic sites of several enzymes involved in glycerophospholipid-, triacylglycerol-sphingolipid and GPI anchor biosynthesis to the cytosol or ER lumen. For enzymes using cytosolically made substrates and having the catalytic site in the ER we need to understand how these substrates are transported across the ER membrane.