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Apparatus for Colonoscopy and Endoscopic Manipulations in Mice

English title APPARATUS FOR COLONOSCOPY AND ENDOSCOPIC MANIPULATIONS IN MICE
Applicant Krebs Philippe
Number 145006
Funding scheme R'EQUIP
Research institution Institut für Pathologie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Immunology, Immunopathology
Start/End 01.09.2013 - 31.08.2014
Approved amount 54'000.00
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All Disciplines (6)

Discipline
Immunology, Immunopathology
Pathophysiology
Molecular Biology
Surgery
Cellular Biology, Cytology
Experimental Cancer Research

Keywords (7)

3R principles of animal experimentation; Time-course analysis; Colon biopsies from live mice; Colorectal cancer ; Colonoscopy in live mice; Replacement of colonic tissue; Inflammatory bowel disease

Lay Summary (French)

Lead
Dans le cadre de ce projet, nous allons employer un endoscope miniaturisé pour suivre la progression de pathologies intestinales (inflammatoires ou liées au cancer du côlon) dans des modèles de souris. L’endoscope va aussi nous permettre d’évaluer l’emploi d’un nouveau polymère pour assister la guérison de la paroi intestinale, par exemple après une intervention chirurgicale.
Lay summary

L’endoscopie est une technique très fréquemment utilisée et qui permet d’ausculter l’appareil digestif de patients en vue de déceler certaines pathologies ou de prendre des biopsies in situ. Récemment, des endoscopes miniaturisés ont spécialement été conçus pour un emploi similaire, dans des souris.

Nous comptons employer un endoscope pour souris à la Faculté de Médecine de l’Université de Berne pour les projets suivants : i) étude de la régulation de la réponse immunitaire de l’intestin dans des modèles de maladie inflammatoire de l'intestin ; ii) investigation du rôle d’une protéine impliquée dans la régulation post-transcriptionnelle de l’expression génique dans l’intestin et son influence pour le développement de lésions inflammatoires et/ou cancéreuses ; iii) évaluation de l’emploi d’un nouveau polymère pour assister la guérison de la paroi intestinale.

Du fait que l’endoscopie n’est que très peu invasive, la méthode peut être parfaitement employée pour évaluer le développement de lésions intestinales sur plusieurs jours ou semaines, dans une même souris (préalablement anesthésiée avant toute manipulation). Ainsi, grâce à l’emploi de cet endoscope, nous pensons pouvoir réduire le nombre de souris nécessaires à nos expériences.

Direct link to Lay Summary Last update: 07.08.2013

Lay Summary (English)

Lead
We will use a miniature endoscope to follow the progression of intestinal disease (inflammation and/or colorectal cancer) in mouse models. The endoscope will be also help evaluating the use of a biomaterial for intestinal wound healing in mice. Because endoscopy is minimally invasive and permits to track disease progression or healing process over time and in single (anesthetized) mice, it will also contribute in reducing the number of animals we use in experiments.
Lay summary

Endoscopy is a widely used technique in patients that allows non-invasive examination of the intestine for the diagnosis of several (gastro)intestinal disorders. Recently, miniature devices have been designed to allow endoscopy in live, anesthetized mice, including imaging and the sampling of tissue biopsies.

Such equipment will use for the following projects at the Medical Faculty of the University of Bern i) the study of the regulation of the intestinal immune responses in mouse models of inflammatory bowel disease; ii) an investigation of the role of a protein important for post-transcriptional gene regulation for the development of intestinal inflammation and progression of colorectal cancer in experimental mouse models; iii) the use of a composite biomaterial for the replacement of damaged or removed colonic tissue in mice. An endoscope will thus be particularly advantageous for evaluating, over time, the progression or regression of pathological manifestations or wound healing in single mice. Furthermore, it will permit a delineation of the optimal time point for tissue harvesting (for in-depth cellular or molecular investigations), thereby also allowing a reduction of the number of animals in experimentation.

Direct link to Lay Summary Last update: 07.08.2013

Responsible applicant and co-applicants

Publications

Publication
The ESRP1-GPR137 axis contributes to intestinal pathogenesis
Mager Lukas Franz, Koelzer Viktor, Stuber Regula, Thoo Lester, Keller Irene, Koeck Ivonne, Langenegger Maya, Simillion Cedric, Pfister Simona, Faderl Martin, Genitsch Vera, Tcymbarevich Irina, Juillerat Pascal, Li Xiaohong, Xia Yu, Karamitopoulou Eva, Lyck Ruth, Zlobec Inti, Hapfelmeier Siegfried, Bruggmann Rémy, McCoy Kathy, MacPherson Andrew J, Mueller Christoph, Beutler Bruce, Krebs Philippe (2017), The ESRP1-GPR137 axis contributes to intestinal pathogenesis, in eLife, 6, 1.
CD4 T cells are required for both development and maintenance of disease in a new mouse model of reversible colitis
Brasseit J, Althaus-Steiner E, Faderl M, Dickgreber N, Saurer L, Genitsch V, Dolowschiak T, Li H, Finke D, Hardt W-D, McCoy K D, Macpherson A J, Corazza N, Noti M, Mueller C (2016), CD4 T cells are required for both development and maintenance of disease in a new mouse model of reversible colitis, in Mucosal Immunology, 9(3), 689-701.
The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice.
Mertz KD, Mager LF, Wasmer MH, Thiesler T, Koelzer VH, Ruzzante G, Joller S, Murdoch JR, Brümmendorf T, Genitsch V, Lugli A, Cathomas G, Moch H, Weber A, Zlobec I, Junt T, Krebs P (2016), The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice., in Oncoimmunology, 5(1), 1.

Collaboration

Group / person Country
Types of collaboration
Pascal Juillerat, MD, Gastroenterologist, Bern University Hospital Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Prof. Bruce Beutler / UT Southwestern, Dallas United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Markus Neurath, Medical Clinic, University of Erlangen-Nuremberg, Erlangen Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Macpherson AJ, Gastroenterologist, Bern University Hospital, Switzerland Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Associated projects

Number Title Start Funding scheme
138392 Pathophysiology of intestinal intraepithelial lymphocytes: Functional heterogeneity and plasticity of CD8aa TCRab T cells 01.11.2011 Project funding (Div. I-III)
138188 Molecular dissection of microbe-induced immunopathlogy 01.09.2012 Project funding (Div. I-III)
136286 Recovery of intestinal homeostasis after microbial or immunological challenge 01.12.2011 Sinergia
163086 mRNA splicing and epithelial integrity 01.04.2016 Project funding (special)
134274 Schweizer IBD Kohorten Studie 01.04.2011 Cohort Studies Large

Abstract

Endoscopy is a widely used technique in patients that allows non-invasive examination of the intestine for the diagnosis of several (gastro)intestinal disorders. Recently, several research groups interested in intestinal pathology have started to use miniature endoscopes in live mice. We apply here for an apparatus for miniature endoscopy in mice that includes the entire setting for high quality live imaging, a miniature forceps for colonic biopsies and an anesthesia apparatus with heated pad, as all manipulations on mice will be done under narcosis.Such equipment will be of invaluable help for the respective projects of the applicants that involve i) the study of the regulation of the intestinal immune responses, both in a normal and pathologic context, and mainly in mouse models of inflammatory bowel disease (IBD); ii) an investigation of the role of a splicing regulator for the development of intestinal inflammation and progression of colorectal cancer in experimental mouse models; iii) the use of a composite biomaterial for the replacement of damaged or removed colonic tissue in mice. An endoscope will thus be particularly advantageous for evaluating, over time, the progression or regression of pathological manifestations or wound healing in single mice. Furthermore, it will permit a delineation of the optimal time point for tissue harvesting (for in-depth cellular or molecular investigations), thereby also allowing a reduction of the number of animals in experimentation.
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