3R principles of animal experimentation; Time-course analysis; Colon biopsies from live mice; Colorectal cancer ; Colonoscopy in live mice; Replacement of colonic tissue; Inflammatory bowel disease
Mager Lukas Franz, Koelzer Viktor, Stuber Regula, Thoo Lester, Keller Irene, Koeck Ivonne, Langenegger Maya, Simillion Cedric, Pfister Simona, Faderl Martin, Genitsch Vera, Tcymbarevich Irina, Juillerat Pascal, Li Xiaohong, Xia Yu, Karamitopoulou Eva, Lyck Ruth, Zlobec Inti, Hapfelmeier Siegfried, Bruggmann Rémy, McCoy Kathy, MacPherson Andrew J, Mueller Christoph, Beutler Bruce, Krebs Philippe (2017), The ESRP1-GPR137 axis contributes to intestinal pathogenesis, in eLife
, 6, 1.
Brasseit J, Althaus-Steiner E, Faderl M, Dickgreber N, Saurer L, Genitsch V, Dolowschiak T, Li H, Finke D, Hardt W-D, McCoy K D, Macpherson A J, Corazza N, Noti M, Mueller C (2016), CD4 T cells are required for both development and maintenance of disease in a new mouse model of reversible colitis, in Mucosal Immunology
, 9(3), 689-701.
Mertz KD, Mager LF, Wasmer MH, Thiesler T, Koelzer VH, Ruzzante G, Joller S, Murdoch JR, Brümmendorf T, Genitsch V, Lugli A, Cathomas G, Moch H, Weber A, Zlobec I, Junt T, Krebs P (2016), The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice., in Oncoimmunology
, 5(1), 1.
Endoscopy is a widely used technique in patients that allows non-invasive examination of the intestine for the diagnosis of several (gastro)intestinal disorders. Recently, several research groups interested in intestinal pathology have started to use miniature endoscopes in live mice. We apply here for an apparatus for miniature endoscopy in mice that includes the entire setting for high quality live imaging, a miniature forceps for colonic biopsies and an anesthesia apparatus with heated pad, as all manipulations on mice will be done under narcosis.Such equipment will be of invaluable help for the respective projects of the applicants that involve i) the study of the regulation of the intestinal immune responses, both in a normal and pathologic context, and mainly in mouse models of inflammatory bowel disease (IBD); ii) an investigation of the role of a splicing regulator for the development of intestinal inflammation and progression of colorectal cancer in experimental mouse models; iii) the use of a composite biomaterial for the replacement of damaged or removed colonic tissue in mice. An endoscope will thus be particularly advantageous for evaluating, over time, the progression or regression of pathological manifestations or wound healing in single mice. Furthermore, it will permit a delineation of the optimal time point for tissue harvesting (for in-depth cellular or molecular investigations), thereby also allowing a reduction of the number of animals in experimentation.