transcription factor; ChIP-SEQ; motility; compartmentalization; G1-phase; asymmetric division; cell-cycle control; pho system; Caulobacter
Joshi Kamal Kishore, Berge Matthieu, Radhakrishnan Sunish Kumar, Viollier Patrick Henri, Chien Peter (2015), An Adaptor Hierarchy Regulates Proteolysis during a Bacterial Cell Cycle, in CELL
, 163(2), 419-431.
De Nisco Nicole J., Ferri Lorenzo, Penterman Jon, Fioravanti Antonella, Brilli Matteo, Mengoni Alessio, Bazzicalupo Marco, Viollier Patrick H., Walker Graham C., Biondi Emanuele G. (2015), Cell Cycle Control by the Master Regulator CtrA in Sinorhizobium meliloti, in PLOS GENETICS
, 11(5), 1-24.
Sanselicio Stefano, Viollier Patrick (2015), Convergence of Alarmone and Cell Cycle Signaling from Trans-Encoded Sensory Domains., in MBio
, 6(5), 1-10.
Sanselicio Stefano, Berge Matthieu, Theraulaz Laurence, Radhakrishnan Sunish Kumar, Viollier Patrick H. (2015), Topological control of the Caulobacter cell cycle circuitry by a polarized single-domain PAS protein, in NATURE COMMUNICATIONS
, 6, 1-14.
Murray Sean R., Viollier Patrick H. (2015), Versatility of global transcriptional regulators in alpha-Proteobacteria: from essential cell cycle control to ancillary functions, in FEMS MICROBIOLOGY REVIEWS
, 39(1), 120-133.
Fiebig Aretha, Herrou Julien, Fumeaux Coralie, Radhakrishnan Sunish K., Viollier Patrick H., Crosson Sean (2014), A Cell Cycle and Nutritional Checkpoint Controlling Bacterial Surface Adhesion, in PLOS GENETICS
, 10(1), 1-14.
Ardissone Silvia, Fumeaux Coralie, Bergé Matthieu, Beaussart Audrey, Théraulaz Laurence, Radhakirishnan Sunish, Viollier Patrick (2014), Cell cycle constraints on capsulation and bacteriophage susceptibility, in Elife
Fumeaux Coralie, Radhakrishnan Sunish Kumar, Ardissone Silvia, Theraulaz Laurence, Frandi Antonio, Martins Daniel, Nesper Jutta, Abel Soeren, Jenal Urs, Viollier Patrick H. (2014), Cell cycle transition from S-phase to G1 in Caulobacter is mediated by ancestral virulence regulators, in NATURE COMMUNICATIONS
, 5, 1-14.
Murray Sean M., Panis Gael, Fumeaux Coralie, Viollier Patrick H., Howard Martin (2013), Computational and Genetic Reduction of a Cell Cycle to Its Simplest, Primordial Components, in PLOS BIOLOGY
, 11(12), 1-16.
Davis Nicole J., Cohen Yaniv, Sanselicio Stefano, Fumeaux Coralie, Ozaki Shogo, Luciano Jennifer, Guerrero-Ferreira Ricardo C., Wright Elizabeth R., Jenal Urs, Viollier Patrick. H. (2013), De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle (vol 27, pg 2049, 2013), in GENES & DEVELOPMENT
, 27(20), 2292-2292.
Fioravanti Antonella, Fumeaux Coralie, Bompard Coralie, Brilli Matteo, Frandi Antonio, Castric Vincent, Villeret Vincent, Viollier Patrick H., Biondi Emanuele G. (2013), DNA Binding of the Cell Cycle Transcriptional Regulator GcrA Depends on N6-Adenosine Methylation in Caulobacter crescentus and Other Alphaproteobacteria, in PLOS GENETICS
, 9(5), 1-16.
Panis Gael, Lambert Christophe, Viollier Patrick H. (2012), Complete Genome Sequence of Caulobacter crescentus Bacteriophage phi CbK, in JOURNAL OF VIROLOGY
, 86(18), 10234-10235.
In contrast to common belief, bacteria coordinate transcription of selected genes with cell cycle progression. One cell cycle event exploited for coordination is the physical act of cell compartmentalization that allows different transcriptional programs to be activated in each daughter cell compartment. Despite potentially being wide-spread in the a-proteobacteria and perhaps bacteria in general, the underlying molecular and regulatory mechanisms are not well understood. Here we identify and study the molecular determinants that control the activation of G1-phase (and repression of S-phase) promoters in the Caulobacter crescentus swarmer cell chamber after compartmentalization. C. crescentus is an ideal model system for such studies because of its genetic tractability and physical synchronizability. Moreover, at the end of each cell cycle, the closure of the division septum gives rise to two cellular chambers in which different transcriptional programs are activated. While in the swarmer cell chamber G1-phase specific promoters fire, in the stalked cell chamber S-phase promoters are activated. We identify(ied) factors that control this promoter selectivity and we will explore the genetic relationships among these determinants to understand how the individual regulatory layers integrate into a robust system for compartment-specific transcriptional activation. Included in this analysis is a phosphate sensory system that interacts genetically with a master transcriptional regulator of the cell cycle. We will characterize the functional interplay amongst these transcriptional regulators at the biochemical level with wild-type and mutant proteins. These experiments will primarily rely on a combination of powerful forward genetics, cutting-edge chromatin-immunoprecipitation/deep-sequencing (ChIP-SEQ) and in vitro competition experiment assayed by electrophoretic mobility shift and footprinting.