hyphenation; biotechnology of phytopharmaceuticals; phytochemical analysis; High Performance Thin Layer Chromatography (HPTLC ; plant tissue culture; medicinal plants; bioactivity screening; pharmacognosy; bioassay-guided cultivation; ex-situ conservation; in-vitro cultivation
Todorova Milka, Trendafilova Antoaneta, Danova Kalina, Simmons Luke, Wolfram Evelyn, Meier Beat, Riedl Rainer, Evstatieva Luba (2015), Highly oxygenated sesquiterpenes in Artemisia alba Turra., in Phytochemistry
, 110, 140-149.
Koleva P, Wolfram E, Pedrussio S, Raynova Y, Estatieva L, Danova K (2015), In vitro culture development and polyphenolics production of Artemisia alba Turra., in Bio. Sci. Biotechol.
, SE/Online, 131-136.
Danova K (2015), Potential of the Balkan Flora as a Source of Prospective Hypericum Genotypes for the Conventional and Biotechnological Delivery of Phytopharmaceuticals., in Davis Howard R (Ed) (ed.), Nova Science Publishers, USA, 19-52.
Wolfram E, Danova K, Könye R, Pedrussio S, Aneva L, Evstatieva, Bräm S, Meier B (2014), Bioautographic screening of Xanthine Oxidase inhibition and antioxidant activities for bioprocess control of in vitro cultivated medicinal and aromatic plants of the Balkan region, in Planta medica
, 80, P2B62.
Treneva G., Markovska Y., Wolfram E., Danova K. (2014), Effect of plant growth regulators on growth patterns and enzymatic antioxidant activities in Hypericum calycinum shoot cultures, in Bulgarian Journal of Agricultural Science
, 20(1), 46-50.
Y Markovska Y Raynova K Idakieva M Todorova E Wolfram K Danova (2013), Enzymatic activity and physiological status affect essential oils and polyphenolics production in Artemisia alba tissue cultures, in Planta medica
, 79, PN20.
M Todorova A Trendafilova L Evstatieva L Simmons E Wolfram K Danova (2013), Highly oxygenated sesquiterpenes from the aerial parts of Artemisia alba Turra, in Planta medica
, 79, PI104.
Mehandzhiyski A Batovska D Dimitrov D Evstatieva L Danova K (2013), Nitric Oxide-Scavenging Activity Of In Vitro Cultured Balkan Medicinal And Aromatic Plants, in BJAS Bulgarian Journal of Agricultural Science
, 19(2), 31-34.
K Danova L Evstatieva M Todorova A Trendafilova E Wolfram (2013), Optimization of in vitro culture system for biomass and polyphenolics production in Inula britannica and Sideritis scardica Sofia 2 cultivar, in Planta medica
, 79, PN21.
E Wolfram L Hostettler S Peter M Todorova A Trendafilova K Danova (2013), Phytochemical assessment of the effect of stimulated in vitro multiplication on the metabolic profile of in vitro cultured Hypericum richeri and Artemisia alba, in Planta medica
, 79, PN119.
Raynova Y Markovska Y Idakieva K Wolfram E Danova K. (2013), Relations between enzymatic and non-enzymatic antioxidant defence involved in polyphenolics production of Artemisia alba in vitro, in Proceedings of Workshop of Ecology, Union of Scientists
, SofiaBulgarian Academy of Sciences, Sofia.
Krumova S Motyka V Dobrev P Todorova M Trendafilova A Evstatieva L Danova K (2013), Terpenoid profile of Artemisia alba is related to endogenous cytokinins in vitro, in Bulgarian Journal of Agricultural Science
, 19(2), 26-30.
Danova Kalina, Biotechnological Utilization of the Indigenous Biosynthetic Capacity of Medicinal and Aromatic Plants: Experience in the Genera Hypericum Pulsatilla and Essential Oil Bearing Artemisia alba Characteri, in Govil J.N. (ed.), Studium Press LLC, Houston, USA, 355-392.
Todorova M, Trendafilova A, Krumova S, Idakieva K, Genova V, Markovska Y, Raynova Y, Estatieva L, Wolfram E, Danova K, Interdisciplinary interaction for the biotechnological development of Balkan medicinal plant species, in Proceedings of Seminar of Ecology, Union of Scientists,
, Sofia, BulgarienBulgarian Academy of Sciences, Sofia.
Raynova Y, Markovska Y, Idakieva K, Wolfram E, Danova K, Relations between enzymatic and non-enzymatic antioxidant defence involved in polyphenolics production of Artemisia alba in vitro, in Proceedings of Seminar of Ecology, Union of Scientists,
, Sofia, BulgariaBulgarian Academy of Sciences, Sofia.
Medicinal plants are considered to be a group of special interest due to their importance for human survival and potential of overexploitation.According to the World Health Organization, today about 80% of Earth population still relies mainly on medicinal plants as traditional treatment method. Also, about 25% of the prescribed medications and 50% of the over-the-counter drugs in the industrialized countries are of plant origin (including 52% of the anti-inflammatory and 51% of the anti-tumor products). In the era of increasing drug resistance of pathogenic microorganisms towards the long-lived applied traditional antibiotics, much hope is given to plant derived active constituents due to their huge structural diversity and high number of pharmacologically active functional groups in the molecule, making it less possible to develop resistance. Although a great number of active molecules are nowadays derived through the means of chemical synthesis, for a large number of products this is still impossible, due to their complicated nature, or synthetic processes are far too expensive and economically disadvantageous. Therefore, pharmaceutical industry still relies largely on substances derived from plant raw material by extraction and purification processes. Crucial point in industrial scale production is the dependence of results on the quality of the plant raw material which can vary due to variations of natural parameters such as soil, precipitation, climate as well as agricultural manipulations and post-harvest treatment. This is the reason, why especially the pharmaceutical industry is seeking sources of natural products, which offer raw materials of defined and constant quality.Europe’s varied geography and climate provide a vast range of habitats that support more than 12’500 vascular plants. Many major and minor crops have wild relatives in Europe and have been developed and domesticated in the region. Thus, Europe’s flora is one of the best known in the world and has been shaped by human interventions for many hundreds of years. Threats facing medicinal and aromatic plant species in Europe are very similar across the world: largely unmonitored trade, over-exploitation, destructive harvesting techniques, as well as habitat loss and habitat changes, resulting in diminution of population sizes, decrease in genetic diversity and finally to the extinction of the species. An additional impact in the countries of the former Eastern Bloc has been the deregulation of state-controlled commerce resulting in the increase of wild collection. PhytoBalk will support a broad interdisciplinary research to establish ex situ germplasm collection of valuable Balkan medicinal plant species. This will enable the production of biologically active plant extracts, fractions and individual compounds away from the wild habitats in a flexible and standardized manner through modification of culture conditions and establishment of highly productive cell, tissue or organ lines in vitro. A broad in vitro screening of the therapeutic potential for the treatment of an array of socially significant ailments such as inflammation, aging, gout, dementia, Alzheimer’s disease, diabetes and obesity will be performed. The estrogenic and anti-tumor activity will also be tested. The bacteriostatic/bactericidal and fungicidal potential of the obtained products will be estimated on pathogenic indicator microorganisms. Plant species, which will be investigated in PhytoBalk, include representatives of several genera and were chosen as a result of an ethnobotanic survey and are expected to yield plant derived products with defined pharmacological potential. They include species of conservational interest not only for the Balkan region, but for Switzerland as well. Experimental work of PhytoBalk will result in establishment of standardized protocols for the ex situ conservation of the investigated species and biotechnological derivation of the most prospective therapeutically active products selected by a wide array of bioassays foreseen in this project. These acquisitions will bring scientifically based new knowledge and serve as a model for also other valuable medicinal plant species, representative of the floras of the two countries in future collaborations.Thus, the planned research sets premises for the initial steps of prospective drug candidate discovery and hence falls into the scope of the “Drug development” topic, but is also concerning ex situ conservational issues of the “Ecological forestry/farming and waste management” topic of the Bulgarian-Swiss Research Programme 2011-2016 call. HypothesisPlant metabolic profile is genetically pre-determined, but its qualitative and quantitative characteristics are dynamic and can vary strikingly depending on the complex interrelations of the plant organism with its surrounding environment. Therefore, by controlling culture conditions in vitro, it is possible to target the production of plant biomass with desired properties. Reliable bioprocess control will be granted by the utilization of rapid and efficient analytical and bioassay methods.