Microfluidics; Pulmonary fibrosis; Hypoxia; Lung on Chip; Hepatic growth factor; Alveolar-capillary model; In-vitro model; Wound-healing; Bioartificial membrane; Air-liquid interface; Epithelial microinjuries
Felder Marcel, Sallin Pauline, Barbe Laurent, Haenni Beat, Gazdhar Amiq, Geiser Thomas, Guenat Olivier (2012), Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium., in Lab Chip
, 12, 640.
Stucki AO1 Stucki JD Hall SR Felder M Mermoud Y Schmid RA Geiser T Guenat OT., A lung-on-a-chip array with an integrated bio-inspired respiration mechanism, in Lab Chip
Wick Peter, Chortarea Savvina, Guenat Olivier, Roesslein Matthias, Stucki Janick, Hirn Stephanie, Petri-Fink Alke, Rothe-Rutishauser Barbara, In vitro-ex vivo model systems for nanosafety assessment, in European Journal of Nanomedicine
, 7(3), 169-179.
Felder Marcel, Stucki Andreas, Stucki Janick, Geiser Thomas, Guenat Olivier, Potential of microfluidic lung epithelial wounding: towards in vivo-like alveolar microinjuries, in Integrative Biology
Pulmonary fibrosis is a chronic and progressive lung disease, whose deadliest and most common form is called idiopathic pulmonary fibrosis (IPF). Although the pathogenesis of IPF is incompletely understood, it is suggested that it is primarily a disease of abnormal alveolar wound repair and remodeling after repeated epithelial microinjuries. Fundamental understanding of the reepithelialization requires advanced alveolar-capillary in vitro models that best reproduce the in vivo microenvironment. The objective of this project is therefore to develop a microfluidic based lung-on-chip device that has the capability to mimic the in vivo conditions of the alveolar-capillary barrier, including the simulation of the respiratory movements of the lung. This novel tool is particularly designed to analyze lung injury, regeneration and repair. For this, the epithelial layer of the bioartificial alveolar membrane, ultimately made of human primary cells, will be chemically injured to imitate in vivo fibrosis. The restoration by the epithelial layer will be investigated by accurate delivery of hepatic growth factor (HGF) and/or by co-culturing bone marrow stromal cells, which are expected to contribute to alveolar repair in the injured lung.