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3D Ultramicroscopy for biological imaging

English title 3D Ultramicroscopy for biological imaging
Applicant Stein Jens Volker
Number 139232
Funding scheme R'EQUIP
Research institution Theodor Kocher Institut Medizinische Fakultät Universität Bern
Institution of higher education University of Berne - BE
Main discipline Immunology, Immunopathology
Start/End 01.12.2011 - 30.11.2012
Approved amount 90'800.00
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All Disciplines (2)

Discipline
Immunology, Immunopathology
Neurophysiology and Brain Research

Keywords (1)

Laser sheet microscopy

Lay Summary (English)

Lead
Lay summary

Ultramicroscopy (also known as selective plane illumination microscopy, or SPIM) is a novel technique that allows optical sectioning and 3D reconstruction of large, centimeter sized, fluorescently labeled biological specimen with (sub)micrometer resolution. It is based on light sheet illumination and perpendicular fluorescence detection in biological specimen that are made transparent by a clearing procedure. This technique is very versatile and can be applied in any biological specimen. We have set up the first commercially available Ultramicroscope in Switzerland and adapted it to biological questions in immunology and neurobiology. The Ultramicroscope is uniquely suited for the investigation of rare immune cells during adaptive immune responses, and the anatomy of neuronal networks in whole brains. The Ultramicroscopy setup will form part of the “Microscopy Imaging Center” (MIC) platform already established at the University of Bern, thus being available to interested researchers inside and outside of the University of Bern.

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Publications

Publication
Light sheet fluorescence microscopy for in situ cell interaction analysis in mouse lymph nodes.
Abe Jun, Ozga Aleksandra J, Swoger Jim, Sharpe James, Ripoll Jorge, Stein Jens V (2016), Light sheet fluorescence microscopy for in situ cell interaction analysis in mouse lymph nodes., in Journal of immunological methods, 431, 1-10.
pMHC affinity controls duration of CD8+ T cell-DC interactions and imprints timing of effector differentiation versus expansion.
Ozga Aleksandra J, Moalli Federica, Abe Jun, Swoger Jim, Sharpe James, Zehn Dietmar, Kreutzfeldt Mario, Merkler Doron, Ripoll Jorge, Stein Jens V (2016), pMHC affinity controls duration of CD8+ T cell-DC interactions and imprints timing of effector differentiation versus expansion., in The Journal of experimental medicine, 10.

Collaboration

Group / person Country
Types of collaboration
Prof. Burkhard Ludewig, Kantonsspital St. Gallen Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. James Sharpe, Centre for Genomic Regulation Spain (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Imaging the Immune System Talk given at a conference 3D Lymph node structure 24.09.2016 Rehovot, Israel Stein Jens Volker;
1st European chemokine conference Talk given at a conference Imaging the Immune system 25.06.2015 Villars-sur-Ollon, Switzerland Stein Jens Volker;
Imaging the Immune System Talk given at a conference Lymphoid organ imaging 30.10.2014 Milan, Italy Stein Jens Volker;
French Society of Immunology Meeting Talk given at a conference 3D lymphoid organ imaging 15.11.2013 Paris, France Stein Jens Volker;
3rd International Lymphoid tissue Meeting, Rotterdam, Netherlands Talk given at a conference 3D Lymph node imaging 25.09.2013 Rotterdam, Netherlands Stein Jens Volker;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Gentage Talk 05.12.2012 Schaffhausen, Switzerland Stein Jens Volker;


Awards

Title Year
SNF postdoctoral fellowship for Aleksandra J. Ozga (now at Harvard Medical School) 2016

Associated projects

Number Title Start Funding scheme
128415 Dendritic excitability and synaptic plasticity of cortical neurons under physiological and pathological conditions of neuropathic pain 01.07.2010 SNSF Professorships
125447 Examining the function of lymphoid organ structure during antiviral immune responses using microscopic and mesoscopic imaging 01.06.2009 Sinergia
135649 Investigating the molecular factors controlling lymphocyte motility and activation by in vivo imaging 01.04.2011 Project funding (Div. I-III)

Abstract

Ultramicroscopy (also known as selective plane illumination microscopy, or SPIM) is a novel technique that allows optical sectioning and 3D reconstruction of large, centimeter sized, fluorescently labeled biological specimen with (sub)micrometer resolution. It is based on light sheet illumination and perpendicular fluorescence detection in biological specimen that are made transparent by a clearing procedure. This technique is very versatile and can be applied to any biological specimen. We propose to set up the first commercially available Ultramicroscope in Switzerland and to adapt it to biological questions in immunology and neurobiology. The Ultramicroscope is uniquely suited for the investigation of rare immune cells during adaptive immune responses, and the anatomy of neuronal networks in whole brains. The Ultramicroscopy setup will form part of the “Microscopy Imaging Center” (MIC) platform already established at the University of Bern, thus being available to interested researchers on a local, national and international level.
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