Dicer; RNA interference; non-coding RNA; Epigenetics; Genome regulation; Gene silencing
Tropberger Philipp, Pott Sebastian, Keller Claudia, Kamieniarz-Gdula Kinga, Caron Matthieu, Richter Florian, Li Guohong, Mittler Gerhard, Liu Edison T., Buehler Marc, Margueron Raphael, Schneider Robert (2013), Regulation of Transcription through Acetylation of H3K122 on the Lateral Surface of the Histone Octamer, in CELL
, 152(4), 859-872.
Stunnenberg Rieka, Bühler Marc (2012), A detour to mature., in Molecular cell
, 46(6), 719-21.
Bühler M, Hiller S (2012), Dynamic nature of heterochromatin highlighted by a HP1Swi6- dependent gene silencing mechanism, in Cell Cycle
, 11(21), 3907-3908.
Keller Claudia, Adaixo Ricardo, Stunnenberg Rieka, Woolcock Katrina J, Hiller Sebastian, Bühler Marc (2012), HP1(Swi6) mediates the recognition and destruction of heterochromatic RNA transcripts., in Molecular cell
, 47(2), 215-27.
Woolcock KJ, Stunnenberg R, Gaidatzis D, Hotz H-R, Emmerth S, Barraud P, Bühler M (2012), RNAi keeps Atf1-bound stress response genes in check at nuclear pores, in Genes and Development
, 26(7), 683-692.
Shimada Y, Bühler M (2012), Schizosaccharomyces pombe reporter strains for relative quantitative assessment of heterochromatin silencing, in Yeast
, 29(8), 335-341.
Woolcock Katrina J, Bühler Marc, Nuclear organisation and RNAi in fission yeast., in Current opinion in cell biology
I am applying for a 2 year prolongation of my SNF professorship to follow up on our previous work on the fission yeast Dicer protein. Dicer is a key enzyme in the RNAi pathway and is required for formation of heterochromatin at fission yeast centromeres. The proposed experiments are based on our observations that nuclear retention of Dicer is a prerequisite for a functional heterochromatin assembly pathway and that Dicer accumulates at the nuclear periphery in association with pores. I am proposing yeast genetics, proteomics and genomics experiments that focus on elucidating the molecular details of the nuclear retention mechanism and to investigate the functional relevance of the perinuclear localization of Dicer. This project is a logic continuation of our previous work and will further our understanding of the spatial regulation of RNAi pathways in general.