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Structure, dynamics and interactions of paramagnetic centers characterised by Electrn Paramagnetic Resonance

English title Structure, dynamics and interactions of paramagnetic centers characterised by Electrn Paramagnetic Resonance
Applicant Palivan Cornelia
Number 139133
Funding scheme R'EQUIP
Research institution Physikalische Chemie Departement Chemie Universität Basel
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.12.2011 - 30.11.2012
Approved amount 225'000.00
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Keywords (5)

electron paramagnetic resonance; metal complexes; free radicals; spin labels; paramagnetic centers

Lay Summary (English)

Lead
Paramagnetic centers play a key role in a large variety of chemical, biological or electronic systems and processes, as exemplified by metal complexes, free radicals, biradicals, and valence centers. We characterise paramagnetic centers in complex systems/reactions by Electron Paramagnetic Resonance (EPR) with a new CW EPR spectrometer of Bruker, which completed the Magnetic Resonance research platform of the University of Basel.
Lay summary

Paramagnetic centers play a key role in a large variety of chemical, biological or electronic systems and processes, as exemplified by metal complexes, free radicals, biradicals, and valence centers. A paramagnetic center forms when a system has one or more unpaired electrons, either in a stable state or in an intermediate state generated during a reaction process. Electron Paramagnetic Resonance, EPR allows to characterize in detail paramagnetic centers, such as the metal region in various systems ranging from small complexes to proteins, supramolecular hybrid structures, radical species formed under a multitude of conditions (irradiation processes, metabolic pathways, or chemical reactions) and  even to evaluate the local dynamics in complex systems via spin labeling techniques.

We developed the magnetic resonance platform of the Chemistry Department of the University of Basel with an EPR spectrometer that permits the characterization of paramagnetic centers in materials in all physical states and throughout a range of temperatures from 4 K to 400 K. This spectrometer will facilitate us to shed light on the structure and dynamics of paramagnetic centers in complex systems such as enzyme-based nanoreactors, hierarchically arranged proteins networks, metal-copolymer hybrids or interfaces, or nano- and micro-functional materials.

Direct link to Lay Summary Last update: 30.12.2012

Responsible applicant and co-applicants

Publications

Publication
Specific His6‑tag Attachment to Metal-Functionalized Polymersomes Relies on Molecular Recognition
Tanner Pascal, Ezhevskaya Maria, Nehring Rainer, Van Doorslaer Sabine, Meier Wolfgang, Palivan Cornelia (2012), Specific His6‑tag Attachment to Metal-Functionalized Polymersomes Relies on Molecular Recognition, in The Jounal of Physical Chemistry B, 116(33), 10113-10124.
Light-responsive polymer nanoreactors: a source of reactive oxygen species on demand
Baumann Patric, Balasubramania Vimalkumar, Onaca-Fischer Ozana, Sienkiewicz Andrzej, Palivan Cornelia, Light-responsive polymer nanoreactors: a source of reactive oxygen species on demand, in Nanoscale, 1-8.

Collaboration

Group / person Country
Types of collaboration
Prof. A. Taubert, University of Potsdam Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. K. Fromm, Fribourg University Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
NanoMedicine International Conference 01.11.2012 Shenzhen, China
Swiss soft Days Workshop 29.10.2012 Nestle Research center, Lausanne
TNT Conference 09.09.2012 Madrid, Spain
Swiss soft Days Workshop 01.06.2012 Geneva
Swiss Nanoconvention 23.05.2012 Lausanne
Swiss Soft Days Workshop 13.02.2012 Lausanne, EPFL


Associated projects

Number Title Start Funding scheme
130171 In situ dynamics and fluidics of biological matter 01.04.2010 Project funding (Div. I-III)
172604 BIOINSPIRED FUNCTIONAL PROTEIN-POLYMER SUPRAMOLECULAR NANOASSEMBLIES 01.07.2017 Project funding (Div. I-III)
165859 DESIGN OF NANOREACTORS AND ARTIFICIAL ORGANELLES BASED ON POLYMER SUPRAMOLECULAR ASSEMBLIES 01.05.2016 Project funding (Div. I-III)
126366 Designed Evolution of Artificial Metalloenzymes Based on Streptavidin or Carbonic Anhydrase as Protein Scaffold 01.10.2009 Project funding (Div. I-III)
124406 ANTIOXIDANT NANOREACTORS BASED ON CO-ENCAPSULATION OF ENZYMES IN POLYMERIC VESICLES 01.06.2009 Project funding (Div. I-III)
132498 Applied supramolecular and nanoscale chemistry 01.10.2010 Project funding (Div. I-III)
140302 PROTEIN-POLYMER SUPRAMOLECULAR ASSEMBLIES IN THE DESIGN OF ANTIOXIDANT NANOREACTORS AND PROCESSORS 01.06.2012 Project funding (Div. I-III)

Abstract

Use of Electron Paramagnetic Resonance (EPR) to characterize in detail the geometry and conformation, or the dynamics and interactions of paramagnetic centers is essential to understanding the magnetic comportment of the overall system to which the paramagnetic centers belong. We developed the magnetic resonance platform of the Chemistry Department of the University of Basel with an EPR spectrometer that permits the characterization of paramagnetic centers in materials in all physical states and throughout a range of temperatures from 4 K to 400 K. This spectrometer facilitates us to shed light on the structure and dynamics of paramagnetic centers in complex systems such as enzyme-based nanoreactors, hierarchically arranged proteins networks, metal-copolymer hybrids or interfaces, or nano- and micro-functional materials.
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