Projekt

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Expanding Compound-Specific Isotope Analysis Towards Polar Organic Micropollutants and Chlorine Isotopes

Titel Englisch Expanding Compound-Specific Isotope Analysis Towards Polar Organic Micropollutants and Chlorine Isotopes
Gesuchsteller/in Hofstetter Thomas
Nummer 139111
Förderungsinstrument R'EQUIP
Forschungseinrichtung Eawag
Hochschule Eidg. Anstalt für Wasserversorgung, Abwasserreinigung und Gewässerschutz - EAWAG
Hauptdisziplin Andere Gebiete der Umweltwissenschaften
Beginn/Ende 01.09.2012 - 31.08.2013
Bewilligter Betrag 240'000.00
Alle Daten anzeigen

Alle Disziplinen (2)

Disziplin
Andere Gebiete der Umweltwissenschaften
Andere Gebiete der Erdwissenschaften

Keywords (8)

stable isotopes; compound-specific isotope analyis; organic micropollutant; biodegradation; isotope fractionation; isotope ratio mass spectrometry; contaminated site; aquatic system

Lay Summary (Englisch)

Lead
Lay summary

Compound-specific isotope analysis is one of the key techniques for the identification of contaminant sources and assessment of formation and degradation processes of organic micropollutants. While conservative stable isotope ratios measured in individual compounds allow one to infer their precursor materials and (bio)synthesis pathways, stable isotope fractionation is indicative for the reaction mechanisms and extent of contaminant transformation. CSIA therefore contributes to a profound understanding of the reactive processes determining the fate of chemicals and thus to a proper assessment of the temporal and spatial variability of chemical pollution. Applications of CSIA have been particularly successful if isotope ratios were evaluated for two or more isotopic elements simultaneously. However, instrumental restrictions of the most widespread devices for CSIA, gas chromatography coupled to isotope ratio mass spectrometry (GC/IRMS), largely confine the use of CSIA to the elements C, N, H, and O as well as to compounds amenable to gas chromatography. As a consequence, more comprehensive applications that include more polar and ionic micropollutants and halogen isotopes are currently lacking.

With a multifunctional isotope ratio mass spectrometer, a team of environmental chemists and microbiologists will pursue new avenues for the coupling of liquid chromatography coupled to isotope ratio mass spectrometry (LC/IRMS) and for the analysis of chlorine isotope fractionation in volatile organic contaminants. The LC/IRMS option will be used for the isotopic analysis of polar organic micropollutants and their transformation products as well as for the exploration of isotope fractionation during oxidative transformations of pharmaceuticals and biocides. Chlorine isotope analysis with the dual-inlet IRMS option will enable us to generate reference materials for establishing new procedures of Cl isotope analysis by non-specialized benchtop quadrupol mass spectrometers. The latter will expand our abilities to carry out isotopic analyses beyond the capacity of the requested equipment. To this end, we will investigate the enzymatic mechanisms of isomer-specific hexachlorocyclohexane dechlorination pathways on the basis of C and Cl isotope fractionation analysis. The same isotopic elements will also enable us to use isotope effect as probes for precursor materials and mechanisms responsible for the formation of toxic chlorinated organic disinfection by-products during drinking water treatment.

With the requested instrumentation we will be able to make innovative contributions to emerging applications of CSIA. Moreover, our activities will provide a diverse group of researchers with access to stable-isotope based methods and help to establish new national and international collaborations.

Direktlink auf Lay Summary Letzte Aktualisierung: 21.02.2013

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Publikationen

Publikation
Isotope fractionation associated with the simultaneous biodegradation of multiple nitrophenol isomers by Pseudomonas putida B2
Wijker Reto S., Zeyer Josef, Hofstetter Thomas B. (2017), Isotope fractionation associated with the simultaneous biodegradation of multiple nitrophenol isomers by Pseudomonas putida B2, in Environ. Sci.: Processes Impacts, 19(5), 775-784.
Characterization of Substrate, Cosubstrate, and Product Isotope Effects Associated With Enzymatic Oxygenations of Organic Compounds Based on Compound- Specific Isotope Analysis
Pati Sarah G., Kohler Hans-Peter E., Hofstetter Thomas B., Characterization of Substrate, Cosubstrate, and Product Isotope Effects Associated With Enzymatic Oxygenations of Organic Compounds Based on Compound- Specific Isotope Analysis, in Harris Michael, Anderson Vernon (ed.), Elsevier, Amsterdam.

Zusammenarbeit

Gruppe / Person Land
Formen der Zusammenarbeit
University of Neuchâtel / Daniel Hunkeler Schweiz (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Publikation
- Forschungsinfrastrukturen
- Austausch von Mitarbeitern
Lodz University of Technology / Piotr Paneth Polen (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Austausch von Mitarbeitern
Stockholm University / Örjan Gustafsson, Henry Holmstrand Schweden (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Austausch von Mitarbeitern
Helmholtz Zentrum München / Martin Elsner Deutschland (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Publikation
- Forschungsinfrastrukturen
- Austausch von Mitarbeitern
University of California Davis / Rebakka Parles Vereinigte Staaten von Amerika (Nordamerika)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten
- Publikation
- Austausch von Mitarbeitern

Wissenschaftliche Veranstaltungen

Aktiver Beitrag

Titel Art des Beitrags Titel des Artikels oder Beitrages Datum Ort Beteiligte Personen
Isotopes 2013 - Isotope Effects Across Disciplines Vortrag im Rahmen einer Tagung Compound-Specific Isotope Analysis of the Oxidative Degradation of Nitroaromatic Contaminants by Nitrobenzene Dioxygenase 16.06.2013 Sopot, Polen Hofstetter Thomas; Kohler Hans-Peter;


Kommunikation mit der Öffentlichkeit

Kommunikation Titel Medien Ort Jahr
Print (Buch, Brochuren, Infoblätter) International Innovation International 2013

Auszeichnungen

Titel Jahr
Best poster award, Isotopes 2013 conference 2013

Verbundene Projekte

Nummer Titel Start Förderungsinstrument
104965 Isotope effects in abiotic reduction reactions of chlorinated groundwater contaminants 01.10.2004 Stipendien für fortgeschrittene Forschende
141805 Assessment of micropollutant degradation using multi-element compound-specific isotope analysis 01.07.2013 Sinergia
134720 Using nitrogen isotope fractionation to assess redox reactions of organic contaminants 01.04.2011 Projektförderung (Abt. I-III)
172950 Biodegradation of organic pollutants by mono- and dioxygenation: Insights into rates and mechanisms from isotope effects of oxygen activation and substrate oxygenation 01.03.2018 Projektförderung (Abt. I-III)
153534 Tracking biotransformation of hexachlorocyclohexane isomers by compound specific isotope analysis 01.07.2014 Projektförderung (Abt. I-III)

Abstract

Compound-specific isotope analysis is one of the key techniques for the identification of contam-inant sources and assessment of formation and degradation processes of organic micropollu-tants. While conservative stable isotope ratios measured in individual compounds allow one to infer their precursor materials and (bio)synthesis pathways, stable isotope fractionation is indic-ative for the reaction mechanisms and extent of contaminant (trans)formation. CSIA therefore contributes to a profound understanding of the reactive processes determining the fate of chem-icals and thus to a proper assessment of the temporal and spatial variability of chemical pollu-tion. Applications of CSIA have been particularly successful if isotope ratios were evaluated for two or more isotopic elements simultaneously. However, instrumental restrictions of the most widespread devices for CSIA, gas chromatography coupled to isotope ratio mass spectrometry (GC/IRMS), largely confine the use of CSIA to the elements C, N, H, and O as well as to com-pounds amenable to gas chromatography. As a consequence, more comprehensive applications that include more polar and ionic micropollutants and halogen isotopes are currently lacking.We request funding for a multifunctional isotope ratio mass spectrometer that enables a team of environmental chemists and microbiologists to overcome these instrumental limitations and pursue new avenues for the coupling of liquid chromatography coupled to isotope ratio mass spectrometry (LC/IRMS) and for the analysis of chlorine isotope fractionation in volatile organic contaminants. Our strategy for the use of the requested instrumentation is based on the devel-opment of new analytical methods and their application in new projects. The LC/IRMS option will be used for the isotopic analysis of polar organic micropollutants and their transformation products as well as for the exploration of isotope fractionation during oxidative transformations of pharmaceuticals and biocides. Chlorine isotope analysis with the dual-inlet IRMS option will enable us to generate reference materials for establishing new procedures of Cl isotope analysis by non-specialized benchtop quadrupol mass spectrometers. The latter will expand our abilities to carry out isotopic analyses beyond the capacity of the requested equipment. To this end, we will investigate the enzymatic mechanisms of isomer-specific hexachlorocyclohexane dechlorin-ation pathways on the basis of C and Cl isotope fractionation analysis. The same isotopic ele-ments will also enable us to use isotope effect as probes for precursor materials and mechanisms responsible for the formation of toxic chlorinated organic disinfection by-products during drink-ing water treatment.With the requested instrumentation we will be able to make innovative contributions to emerging applications of CSIA. Moreover, our activities will provide a diverse group of re-searchers with access to stable-isotope based methods and help to establish new national and international collaborations.
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