Chemical Biology; Protein Translocation; Intracellular chemistry; Covalent Protein Tags
Wymann MP, Solinas G (2013), Inhibition of phosphoinositide 3-kinase γ attenuates inflammation, obesity, and cardiovascular risk factors, in Annals of the New York Academy of Sciences
, 1280(1), 44-47.
Wymann MP, Simons K (2013), Membrane dynamics in physiology and disease, in FEBS Journal
, 280(12), 2729-2729.
Schmidt C, Schneble N, Müller JP, Bauer R, Perino A, Marone R, Rybalkin SD, Wymann MP, Hirsch E, Wetzker R (2013), Phosphoinositide 3-kinase γ mediates microglial phagocytosis via lipid kinase-independent control of cAMP, in Neuroscience
, 233, 44-53.
Barthwal Manoj K, Anzinger Joshua J, Xu Qing, Bohnacker Thomas, Wymann Matthias P, Kruth Howard S (2013), Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation., in PloS one
, 8(3), 58054-58054.
Walser Romy, Burke John E, Gogvadze Elena, Bohnacker Thomas, Zhang Xuxiao, Hess Daniel, Küenzi Peter, Leitges Michael, Hirsch Emilio, Williams Roger L, Laffargue Muriel, Wymann Matthias P (2013), PKCβ Phosphorylates PI3Kγ to Activate It and Release It from GPCR Control., in PLoS biology
, 11(6), 1001587-1001587.
Collmann Emilie, Bohnacker Thomas, Marone Romina, Dawson Janet, Rehberg Markus, Stringer Rowan, Krombach Fritz, Burkhart Christoph, Hirsch Emilio, Hollingworth Gregory J, Thomas Matthew, Wymann Matthias P (2013), Transient targeting of phosphoinositide 3-kinase acts as a roadblock in mast cells' route to allergy., in The Journal of allergy and clinical immunology
, 0(0), 0-0.
Erhart Dominik, Zimmermann Mirjam, Jacques Olivier, Wittwer Matthias B., Ernst Beat, Constable Edwin, Zvelebil Marketa, Beaufils Florent, Wymann Matthias P. (2013), Chemical Development of Intracellular Protein Heterodimerizers, in CHEMISTRY & BIOLOGY
, 20(4), 549-557.
Schmidt C, Schneble N, Müller J P, Bauer R, Perino A, Marone R, Rybalkin S D, Wymann M P, Hirsch E, Wetzker R (2013), Phosphoinositide 3-kinase γ mediates microglial phagocytosis via lipid kinase-independent control of cAMP., in Neuroscience
, 233, 44-53.
Harris Stephanie J, Ciuclan Loredana, Finan Peter M, Wymann Matthias P, Walker Christoph, Westwick John, Ward Stephen G, Thomas Matthew J (2012), Genetic ablation of PI3Kγ results in defective IL-17RA signalling in T lymphocytes and increased IL-17 levels., in European journal of immunology
, 42(12), 3394-404.
Wymann Matthias P, Schultz Carsten (2012), The chemical biology of phosphoinositide 3-kinases., in Chembiochem : a European journal of chemical biology
, 13(14), 2022-35.
Fougerat A, Smirnova N F, Gayral S, Malet N, Hirsch E, Wymann M P, Perret B, Martinez L O, Douillon M, Laffargue M (2012), Key role of PI3Kγ in monocyte chemotactic protein-1-mediated amplification of PDGF-induced aortic smooth muscle cell migration., in British journal of pharmacology
, 166(5), 1643-53.
Reichel Christoph A, Puhr-Westerheide Daniel, Zuchtriegel Gabriele, Uhl Bernd, Berberich Nina, Zahler Stefan, Wymann Matthias P, Luckow Bruno, Krombach Fritz (2012), C-C motif chemokine CCL3 and canonical neutrophil attractants promote neutrophil extravasation through common and distinct mechanisms., in Blood
, 120(4), 880-90.
Schaefer T, Zajonz A, Lorentz P, Bohnacker T, Wymann M P, Schweighoffer T (2012), Luminal decoration of blood vessels by activated perivasal mast cells in allergic rhinitis., in Allergy
, 67(4), 510-20.
Anzinger Joshua J, Chang Janet, Xu Qing, Barthwal Manoj K, Bohnacker Thomas, Wymann Matthias P, Kruth Howard S (2012), Murine bone marrow-derived macrophages differentiated with GM-CSF become foam cells by PI3Kγ-dependent fluid-phase pinocytosis of native LDL., in Journal of lipid research
, 53(1), 34-42.
The status of cellular signaling pathways is crucial to switch from normal physiology to disease. Many signaling cascades are triggered by the relocalization of protein and lipid kinases. Based on a novel prototype molecule, we propose here to establish cell permeable, non-interfering, covalently reacting protein dimerizers and suitable translocation systems. The combination of chemical dimerizers, specifically targeted membrane anchors and tagged signaling enzymes will provide a toolbox to investigate the activation of single signaling nodes at defined cellular localizations.