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Assessing metal bioavailability through transcriptome response in aquatic primary producers

Titel Englisch Assessing metal bioavailability through transcriptome response in aquatic primary producers
Gesuchsteller/in Cosio Claudia
Nummer 138254
Förderungsinstrument Projekte
Forschungseinrichtung Institut F.-A. Forel Université de Genève
Hochschule Universität Genf - GE
Hauptdisziplin Andere Gebiete der Umweltwissenschaften
Beginn/Ende 01.03.2012 - 28.02.2015
Bewilligter Betrag 257'063.00
Alle Daten anzeigen

Alle Disziplinen (3)

Disziplin
Andere Gebiete der Umweltwissenschaften
Andere Gebiete der Erdwissenschaften
Molekularbiologie

Keywords (4)

bioavailability,; ecotoxicogenomics; macrophyte; microalgae

Lay Summary (Englisch)

Lead
Lay summary

The proposed project aims to assess the relationship between toxic metal bioavailability and expression of genes in two representative aquatic primary producers: a rooted macrophyte and a green microalgae, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column.

Bioavailability of Cu and two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) will be compared to transcriptome modifications analyzed by RNAseq as well as other endpoints including growth and photosynthesis in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. The specificity of the gene response to each toxicant and the response to their mixtures will be assessed. Genes identified in both organisms in similar conditions will be compared to determine how similar are primary producers’ response to heavy metals.

Direktlink auf Lay Summary Letzte Aktualisierung: 21.02.2013

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Mitarbeitende

Publikationen

Publikation
Towards mechanistic understanding of mercury availability and toxicity to aquatic primary producers
(2014), Towards mechanistic understanding of mercury availability and toxicity to aquatic primary producers, in Chimia, 68, 799-805.
Physiological and proteomic changes in shoots of Elodea nuttallii suggest an important role of cell walls in its high tolerance to Hg and Cd
(2013), Physiological and proteomic changes in shoots of Elodea nuttallii suggest an important role of cell walls in its high tolerance to Hg and Cd, in Journal of Hazardous Materials , 575.
Elodea nuttallii roots effect on bacterial communities and MMHg proportion in an Hg polluted sediment
(2012), Elodea nuttallii roots effect on bacterial communities and MMHg proportion in an Hg polluted sediment, in Plos one, 7(9), e45565.
Effects of macrophytes on the fate of mercury in aquatic systems.
, Effects of macrophytes on the fate of mercury in aquatic systems., in Environmental Toxicology and Chemistry , 1.

Zusammenarbeit

Gruppe / Person Land
Formen der Zusammenarbeit
Swiss Institute fo Bioinformatics Schweiz (Europa)
- vertiefter/weiterführender Austausch von Ansätzen, Methoden oder Resultaten

Wissenschaftliche Veranstaltungen

Aktiver Beitrag

Titel Art des Beitrags Titel des Artikels oder Beitrages Datum Ort Beteiligte Personen
5ème séminaire d’écotoxicologie de l’INRA Vortrag im Rahmen einer Tagung Analyse du transcriptome pour évaluer la biodisponibilité des polluants dans des mélanges chez Elodea nuttallii 25.11.2014 Biarritz, Frankreich Cosio Claudia;
Colloque 2014 de la Société Française d’Ecotoxicologie Fondamentale et Appliquée (SEFA) Vortrag im Rahmen einer Tagung Looking for biomarkers of mercury exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 01.07.2014 Besancon, Frankreich Cosio Claudia;
24rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Interactions between Hg in shallow aquatic environment and macrophytes 11.05.2014 Basel, Schweiz Cosio Claudia;
24rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Uptake kinetics of inorganic and methyl mercury by two representative aquatic primary producers 11.05.2014 Basel, Schweiz Slaveykova-Startcheva Vera; Cosio Claudia; Beauvais-Flueck Rebecca;
24th annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC). Poster Assessment of inorganic and methyl mercury bioaccumulation in two aquatic primary producers 11.05.2014 Basel, Schweiz Cosio Claudia; Beauvais-Flueck Rebecca; Slaveykova-Startcheva Vera;
SWISSPLANT'14 Poster Effects of mercury and UV radiation on the aquatic plant Elodea nuttallii 29.01.2014 Meiringen, Schweiz Cosio Claudia;
DNA watch workshop Vortrag im Rahmen einer Tagung Influence of a macrophyte on bacterial communities in a Hg polluted sediment (Babeni Reservoir, Romania). 05.12.2013 Frasne, Frankreich Cosio Claudia;
International Conference on Mercury as a Global Pollutant (ICMGP) Vortrag im Rahmen einer Tagung Interactions between Hg and the rooted macrophyte Elodea nuttallii in aquatic environment 28.07.2013 Edinburgh, Grossbritannien und Nordirland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Looking for biomarkers of metal exposure in complex environment by transcriptome analysis in the aquatic plant Elodea nuttallii 12.05.2013 Glasgow, Grossbritannien und Nordirland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Hg bioaccumulation in shoots of the macrophyte Elodea nuttallii: an opportunity for phytoremediation or a risk for the trophic chain? 12.05.2013 Glasgow, Grossbritannien und Nordirland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Interactions between Hg in the aquatic environment and macrophytes 12.05.2013 Glasgow, Grossbritannien und Nordirland Cosio Claudia; Beauvais-Flueck Rebecca;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Poster Mercury accumulation in the submersed plant Elodea nuttallii. 12.05.2013 Glasgow, Grossbritannien und Nordirland Slaveykova-Startcheva Vera; Beauvais-Flueck Rebecca; Cosio Claudia;
SWISSPLANT'13 Symposium Vortrag im Rahmen einer Tagung Interactions between Hg in complex aquatic environments and macrophytes 30.01.2013 Meiringen, Schweiz Cosio Claudia;
scientific workshop “Genericity of responses and adaptation strategies to multiple stresses in organisms of aquatic and terrestrial ecosystems” Vortrag im Rahmen einer Tagung Looking for biomarkers of metal exposure in a complex environment by transcriptome analysis in the aquatic plant Elodea nuttallii 21.11.2012 Paimpont, france, Frankreich Cosio Claudia;
33rd annual North America meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Vortrag im Rahmen einer Tagung Looking for biomarkers of Hg exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 11.11.2012 Long Beach, USA, Vereinigte Staaten von Amerika Cosio Claudia;
16th International Conference of Heavy Metals in the Environment (ICHMET) Vortrag im Rahmen einer Tagung Looking for biomarkers of Hg exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 23.09.2012 Roma, Italie, Italien Cosio Claudia;


Kommunikation mit der Öffentlichkeit

Kommunikation Titel Medien Ort Jahr
Referate/Veranstaltungen/Ausstellungen Nuit de la Science Westschweiz 2014
Referate/Veranstaltungen/Ausstellungen November 12th: visit of 60 high scholars from Switzerland and France (ecotox and bio mol laboratory. International Westschweiz 2013
Referate/Veranstaltungen/Ausstellungen Jetez-vous à l'eau! Portes ouvertes à l'Institut Forel Westschweiz 2012

Verbundene Projekte

Nummer Titel Start Förderungsinstrument
157173 Assessing metal bioavailability through transcriptome response in aquatic primary producers (continuation) 01.03.2015 Projektförderung (Abt. I-III)
126244 Identification of INDEHISCENT target genes in Arabidopsis thaliana 01.10.2010 Resource not found: '2b1f21ac-695f-478c-a2aa-ec513ac50b73'
134627 Metal Bioavailability to Aquatic Photosynthetic Organisms in Changing Environment (BioChEn) 01.09.2011 Projektförderung (Abt. I-III)
152897 Phytoplankton responses to trace metals in complex environment 01.01.2015 Projektförderung (Abt. I-III)

Abstract

Contamination in surface water remains of concern in many sites, despite considerable efforts and investments in wastewater treatment and in prevention of emissions. In this context ecotoxicology - the study of organism’s response to toxicants- is used as a tool to predict the effects of pollution so that the most efficient action can be identified for management of ecosystems. Being able to measure bioavailability to organisms accurately is perceived to play a major role to successful attainment of this goal. Indeed, the bioavailability concepts originates from the fact that detrimental effects in exposed organisms are not caused by the entire amount of chemical compounds present in the environment but only by the fraction that is taken up by the organisms.The proposed project aims to assess the relationship between toxic metal bioavailability and expression of genes in two representative aquatic primary producers: a rooted macrophyte and a green microalgae, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column. This difference in the habitat creates substantial differences with respect to the amount and exposure length of trace metals. Bioavailability of Cu and two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) will be compared to transcriptome modifications analyzed by RNAseq as well as other endpoints including growth and photosynthesis in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. The specificity of the gene response to each toxicant and the response to their mixtures will be assessed. Genes identified in both organisms in similar conditions will be compared to determine how similar are primary producers’ response to heavy metals.The proposed approaches will contribute to improve our understanding of bioavailability and detrimental effects of metals to aquatic primary producers. In particular mode of action and uptake of Cu and Hg will be studied and compared in microalgae and macrophyte. Ecotoxicogenomics has been rarely applied to primary producers until now, moreover macrophytes. This fundamental knowledge might help to develop new tools for the field of ecotoxicology. Indeed, ecotoxicogenomics present exciting opportunities for improved methods of identifying and evaluating potential environmental toxicants, as well as to evaluate their bioavailability and monitoring the effects of exposures to these toxicants.
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