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Assessing metal bioavailability through transcriptome response in aquatic primary producers

English title Assessing metal bioavailability through transcriptome response in aquatic primary producers
Applicant Cosio Claudia
Number 138254
Funding scheme Project funding (Div. I-III)
Research institution Institut F.-A. Forel Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Other disciplines of Environmental Sciences
Start/End 01.03.2012 - 28.02.2015
Approved amount 257'063.00
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All Disciplines (3)

Discipline
Other disciplines of Environmental Sciences
Other disciplines of Earth Sciences
Molecular Biology

Keywords (4)

bioavailability,; ecotoxicogenomics; macrophyte; microalgae

Lay Summary (English)

Lead
Lay summary

The proposed project aims to assess the relationship between toxic metal bioavailability and expression of genes in two representative aquatic primary producers: a rooted macrophyte and a green microalgae, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column.

Bioavailability of Cu and two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) will be compared to transcriptome modifications analyzed by RNAseq as well as other endpoints including growth and photosynthesis in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. The specificity of the gene response to each toxicant and the response to their mixtures will be assessed. Genes identified in both organisms in similar conditions will be compared to determine how similar are primary producers’ response to heavy metals.

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Towards mechanistic understanding of mercury availability and toxicity to aquatic primary producers
Dranguet Perrine, Flueck Rebecca, Regier Nicole, Le Faucheur Severine, Cosio Claudia, Slaveykova Vera (2014), Towards mechanistic understanding of mercury availability and toxicity to aquatic primary producers, in Chimia, 68, 799-805.
Physiological and proteomic changes in shoots of Elodea nuttallii suggest an important role of cell walls in its high tolerance to Hg and Cd
Larras Florianne, Regier Nicole, Planchon Sébastien, Poté John, Renaut Jenny, Cosio Claudia (2013), Physiological and proteomic changes in shoots of Elodea nuttallii suggest an important role of cell walls in its high tolerance to Hg and Cd, in Journal of Hazardous Materials , 575.
Elodea nuttallii roots effect on bacterial communities and MMHg proportion in an Hg polluted sediment
Regier Nicole, Frey Beat, Converse Brandon, Roden Eric, Grosse-Honebrink Alexander, Garcia Bravo Andrea, Cosio Claudia (2012), Elodea nuttallii roots effect on bacterial communities and MMHg proportion in an Hg polluted sediment, in Plos one, 7(9), e45565.
Effects of macrophytes on the fate of mercury in aquatic systems.
Cosio Claudia, Flueck Rebecca, Regier Nicole, Slaveykova Vera, Effects of macrophytes on the fate of mercury in aquatic systems., in Environmental Toxicology and Chemistry , 1.

Collaboration

Group / person Country
Types of collaboration
Swiss Institute fo Bioinformatics Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
5ème séminaire d’écotoxicologie de l’INRA Talk given at a conference Analyse du transcriptome pour évaluer la biodisponibilité des polluants dans des mélanges chez Elodea nuttallii 25.11.2014 Biarritz, France Cosio Claudia;
Colloque 2014 de la Société Française d’Ecotoxicologie Fondamentale et Appliquée (SEFA) Talk given at a conference Looking for biomarkers of mercury exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 01.07.2014 Besancon, France Cosio Claudia;
24rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Interactions between Hg in shallow aquatic environment and macrophytes 11.05.2014 Basel, Switzerland Cosio Claudia;
24rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Uptake kinetics of inorganic and methyl mercury by two representative aquatic primary producers 11.05.2014 Basel, Switzerland Slaveykova-Startcheva Vera; Cosio Claudia; Beauvais-Flueck Rebecca;
24th annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC). Poster Assessment of inorganic and methyl mercury bioaccumulation in two aquatic primary producers 11.05.2014 Basel, Switzerland Cosio Claudia; Beauvais-Flueck Rebecca; Slaveykova-Startcheva Vera;
SWISSPLANT'14 Poster Effects of mercury and UV radiation on the aquatic plant Elodea nuttallii 29.01.2014 Meiringen, Switzerland Cosio Claudia;
DNA watch workshop Talk given at a conference Influence of a macrophyte on bacterial communities in a Hg polluted sediment (Babeni Reservoir, Romania). 05.12.2013 Frasne, France Cosio Claudia;
International Conference on Mercury as a Global Pollutant (ICMGP) Talk given at a conference Interactions between Hg and the rooted macrophyte Elodea nuttallii in aquatic environment 28.07.2013 Edinburgh, Great Britain and Northern Ireland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Looking for biomarkers of metal exposure in complex environment by transcriptome analysis in the aquatic plant Elodea nuttallii 12.05.2013 Glasgow, Great Britain and Northern Ireland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Hg bioaccumulation in shoots of the macrophyte Elodea nuttallii: an opportunity for phytoremediation or a risk for the trophic chain? 12.05.2013 Glasgow, Great Britain and Northern Ireland Cosio Claudia;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Interactions between Hg in the aquatic environment and macrophytes 12.05.2013 Glasgow, Great Britain and Northern Ireland Cosio Claudia; Beauvais-Flueck Rebecca;
23rd annual Europe meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Poster Mercury accumulation in the submersed plant Elodea nuttallii. 12.05.2013 Glasgow, Great Britain and Northern Ireland Slaveykova-Startcheva Vera; Beauvais-Flueck Rebecca; Cosio Claudia;
SWISSPLANT'13 Symposium Talk given at a conference Interactions between Hg in complex aquatic environments and macrophytes 30.01.2013 Meiringen, Switzerland Cosio Claudia;
scientific workshop “Genericity of responses and adaptation strategies to multiple stresses in organisms of aquatic and terrestrial ecosystems” Talk given at a conference Looking for biomarkers of metal exposure in a complex environment by transcriptome analysis in the aquatic plant Elodea nuttallii 21.11.2012 Paimpont, france, France Cosio Claudia;
33rd annual North America meeting of the Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Looking for biomarkers of Hg exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 11.11.2012 Long Beach, USA, United States of America Cosio Claudia;
16th International Conference of Heavy Metals in the Environment (ICHMET) Talk given at a conference Looking for biomarkers of Hg exposure by transcriptome analysis in the aquatic plant Elodea nuttallii 23.09.2012 Roma, Italie, Italy Cosio Claudia;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Nuit de la Science Western Switzerland 2014
Talks/events/exhibitions November 12th: visit of 60 high scholars from Switzerland and France (ecotox and bio mol laboratory. International Western Switzerland 2013
Talks/events/exhibitions Jetez-vous à l'eau! Portes ouvertes à l'Institut Forel Western Switzerland 2012

Associated projects

Number Title Start Funding scheme
157173 Assessing metal bioavailability through transcriptome response in aquatic primary producers (continuation) 01.03.2015 Project funding (Div. I-III)
126244 Identification of INDEHISCENT target genes in Arabidopsis thaliana 01.10.2010 Fellowships for advanced researchers
134627 Metal Bioavailability to Aquatic Photosynthetic Organisms in Changing Environment (BioChEn) 01.09.2011 Project funding (Div. I-III)
152897 Phytoplankton responses to trace metals in complex environment 01.01.2015 Project funding (Div. I-III)

Abstract

Contamination in surface water remains of concern in many sites, despite considerable efforts and investments in wastewater treatment and in prevention of emissions. In this context ecotoxicology - the study of organism’s response to toxicants- is used as a tool to predict the effects of pollution so that the most efficient action can be identified for management of ecosystems. Being able to measure bioavailability to organisms accurately is perceived to play a major role to successful attainment of this goal. Indeed, the bioavailability concepts originates from the fact that detrimental effects in exposed organisms are not caused by the entire amount of chemical compounds present in the environment but only by the fraction that is taken up by the organisms.The proposed project aims to assess the relationship between toxic metal bioavailability and expression of genes in two representative aquatic primary producers: a rooted macrophyte and a green microalgae, representing the aquatic plants and the phytoplankton typically found in the benthic environment and the water column. This difference in the habitat creates substantial differences with respect to the amount and exposure length of trace metals. Bioavailability of Cu and two speciation of Hg (inorganic HgCl2 and organic CH3HgCl) will be compared to transcriptome modifications analyzed by RNAseq as well as other endpoints including growth and photosynthesis in each organism. Bioavailability will be determined by measurement of the adsorbed and internalized metal concentrations at different exposure times. The specificity of the gene response to each toxicant and the response to their mixtures will be assessed. Genes identified in both organisms in similar conditions will be compared to determine how similar are primary producers’ response to heavy metals.The proposed approaches will contribute to improve our understanding of bioavailability and detrimental effects of metals to aquatic primary producers. In particular mode of action and uptake of Cu and Hg will be studied and compared in microalgae and macrophyte. Ecotoxicogenomics has been rarely applied to primary producers until now, moreover macrophytes. This fundamental knowledge might help to develop new tools for the field of ecotoxicology. Indeed, ecotoxicogenomics present exciting opportunities for improved methods of identifying and evaluating potential environmental toxicants, as well as to evaluate their bioavailability and monitoring the effects of exposures to these toxicants.
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