early-onset obsessive-compulsive disorder; serotonin; HTR2A polymorphism; histone deacetylase inhibition; histone methylation; HTR1A; child and adolescent psychiatry
Marinova Zoya, Walitza Susanne, Grünblatt Edna (2017), Effects of oxytocin and arginine vasopressin on the proliferation and differentiation of a serotonergic cell line, in Journal of Neural Transmission
Marinova Zoya, Walitza Susanne, Grünblatt Edna (2014), Real-time impedance-based cell analyzer as a tool to delineate molecular pathways involved in neurotoxicity and neuroprotection in neuronal cell line., in Journal of Visualized Experiments
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Marinova Zoya, Walitza Susanne, Grünblatt Edna (2013), 5-HT2A serotonin receptor agonist DOI alleviates cytotoxicity in neuroblastoma cells: Role of the ERK pathway, in Progress in Neuro-Psychopharmacology & Biological Psychiatry
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Serotonin signaling, including HTR2A and HTR1A, is implicated in obsessive-compulsive disorder (OCD) and depression. The HTR2A -1438G/A polymorphism was shown to be associated with early-onset OCD, but its functional significance is unclear. HTR1A can be functionally antagonistic to HTR2A and its deletion is associated with increased anxiety in mice. Furthermore, epigenetic modifications may affect serotonin signaling. We will test the hypothesis that epigenetic modifications and the -1438G/A HTR2A promoter polymorphism influence serotonin signaling, focusing on HTR2A and HTR1A. We aim to identify downstream targets regulated by HTR2A and HTR1A in rat cortical neurons and determine the role of histone deacetylase inhibition on them. We will also test the -1438G/A HTR2A polymorphism effect on HTR2A target molecules expression and on HTR2A promoter histone acetylation and methylation in lymphocyte cultures from early-onset OCD patients. We expect our results to enrich knowledge about dysregulation of serotonergic signaling and genetic vulnerability to early-onset OCD.