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Regulation of spindle pole asymmetry

Gesuchsteller/in Simanis Viesturs
Nummer 132392
Förderungsinstrument Sinergia
Forschungseinrichtung Institut suisse de recherche expérimentale sur le cancer EPFL SV ISREC
Hochschule EPF Lausanne - EPFL
Hauptdisziplin Zellbiologie, Zytologie
Beginn/Ende 01.01.2011 - 31.12.2013
Bewilligter Betrag 750'000.00
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Keywords (10)

asymmetry; yeast; modelling; image analysis; spindle poles; septation initiation network; cell cycle; cytokinesis; mitosis; S.pombe

Lay Summary (Englisch)

Lead
Lay summary
Asymmetric events within a cell are of fundamental importance in biology. In multicellular organisms, they determine cell fate during development, and are essential for maintaining stem cells in the adult organism; asymmetry of centrosome behaviour and inheritance is also being implicated in an increasing number of cellular and developmental processes. Asymmetry is also important in unicellular organisms; for example, differences in mRNA segregation between cells, or imprinting of individual DNA strands, determine the pattern of mating type switching. The yeast spindle pole body (SPB) is the functional counterpart of the centrosome in mammalian cells. In addition to its role as a microtubule organising center, it also serves as a coordination point for signalling molecules that govern cell division.The fission yeast S. pombe has provided many insights into how cell division is regulated. Cellular division, or cytokinesis, is regulated by a signalling network called the SIN, which is associated with the SPB. Though it may have cytoplasmic roles, all SIN functions require SPB-associated scaffold proteins. In normal cells, the signalling proteins are distributed asymmetrically, associating with the new SPB in late mitosis. This active, asymmetric, configuration of the SIN on the SPBs is generated from an early mitotic, visually symmetrical state. Understanding the rules that govern this in a normal cell, and how this asymmetry is perturbed in mutant cells, or in response to physiological changes in the environment should provide important insights into how SPB asymmetry is generated in this organism. As with other studies of complex processes that have been performed in model systems, our hope is that the insights provided by relatively simple organisms will provide important pointers for how asymmetry of centrosome function is generated in complex organisms. The goal of the research proposed here is to understand the "rules" that govern when SIN proteins, and by implication SPBs, become asymmetric during anaphase B.
Direktlink auf Lay Summary Letzte Aktualisierung: 21.02.2013

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Mitarbeitende

Publikationen

Publikation
An Extended, Boolean Model of the Septation Initiation Network in S.Pombe Provides Insights into Its Regulation.
Chasapi Anastasia, Wachowicz Paulina, Niknejad Anne, Collin Philippe, Krapp Andrea, Cano Elena, Simanis Viesturs, Xenarios Ioannis (2015), An Extended, Boolean Model of the Septation Initiation Network in S.Pombe Provides Insights into Its Regulation., in PloS one, 10(8), 0134214-0134214.
Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN.
Wachowicz Paulina, Chasapi Anastasia, Krapp Andrea, Cano Del Rosario Elena, Schmitter Daniel, Sage Daniel, Unser Michael, Xenarios Ioannis, Rougemont Jacques, Simanis Viesturs (2015), Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN., in Journal of cell science, 128(4), 741-54.
A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis.
Schmitter Daniel, Wachowicz Paulina, Sage Daniel, Chasapi Anastasia, Xenarios Ioannis, Simanis Viesturs, Unser Michael (2013), A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis., in Cell division, 8(1), 6-6.

Wissenschaftliche Veranstaltungen

Aktiver Beitrag

Titel Art des Beitrags Titel des Artikels oder Beitrages Datum Ort Beteiligte Personen
21st International Conference on Intelligent Systems for Molecular Biology and 12th European Conference on Computational Biology (ISMB/ECCB) Poster Modeling the Septation Initiation Network in fission yeast 21.07.2013 Berlin, Deutschland Chasapi Anastasia;
7th international fission yeast meeting Vortrag im Rahmen einer Tagung Analysis of the factors governing the asymmetry of SIN proteins during mitosis 24.06.2013 London, Grossbritannien und Nordirland Simanis Viesturs;
Cell Cycle and Cancer Meeting Poster Characterization of spindle pole asymmetry of the Schizosaccharomyces pombe Septation Initiation Network (SIN) 02.04.2013 Montpellier, Frankreich Wachowicz Paulina;
European Conference of Computational Biology Poster Modeling the septation initiation network in fission yeast 09.09.2012 Basel, Schweiz Chasapi Anastasia;
Unil D-day 2012 Poster Modeling the septation initiation in fission yeast 19.04.2012 Lausanne, Schweiz Chasapi Anastasia;


Selber organisiert

Titel Datum Ort
SIB PhD training network annual retreat 05.03.2011 Bern, Schweiz

Verbundene Projekte

Nummer Titel Start Förderungsinstrument
156769 Regulation of the S.pombe SIN in mitosis and meiosis. 01.04.2015 Projektförderung (Abt. I-III)
138176 Regulation of the S.pombe SIN in mitosis and meiosis. 01.01.2012 Projektförderung (Abt. I-III)
122101 The coordination of mitosis and cytokinesis 01.01.2009 Projektförderung (Abt. I-III)

Abstract

Asymmetric events within a cell are of fundamental importance in biology. In multicellular organisms, they determine cell fate during development, and are essential for maintaining stem cells in the adult organism; asymmetry of centrosome behaviour and inheritance is also being implicated in an increasing number of cellular and developmental processes. Asymmetry is also important in unicellular organisms; for example, differences in mRNA segregation between cells, or imprinting of individual DNA strands, determine the pattern of mating type switching. The yeast spindle pole body (SPB) is the functional counterpart of the centrosome in mammalian cells. In addition to its role as a microtubule organising center, it also serves as a coordination point for signalling molecules that govern cell cycle progression.The fission yeast SIN, which controls the initiation of cytokinesis, represents a relatively simple protein kinase signalling network that is associated with the SPB. Though it may have cytoplasmic roles, all its functions require SPB-associated scaffold proteins. In wild-type cells, the signalling proteins are distributed asymmetrically, associating with the new SPB in anaphase. This active, asymmetric, late-mitotic configuration of the SIN on the SPBs is generated from an early mitotic, visually symmetrical state. Understanding the rules that govern this in a wild-type cell, and how this asymmetry is perturbed in mutant cells, or in response to physiological changes in the environment may provide important insights into how SPB asymmetry is generated in this organism. As with other studies of complex processes that have been performed in model systems, for example regulation of the cell cycle, the expectation is that the insights provided by relatively simple organisms will provide important pointers for how asymmetry of centrosome function is generated in complex organisms. The goal of the research proposed here is to understand the “rules” that govern when SIN proteins, and by implication SPBs, become asymmetric during anaphase B. This study will be undertaken as a collaboration between the laboratories of Professor M. Unser (EPFL), who will develop the tracking and analysis programs to enable analysis of the images, the group of Dr. Ioannis Xenarios (Swiss Institute for Bioinformatics, Vital-IT center), who will develop a qualitative model of the behaviour of the SIN and Professor Viesturs Simanis, who will undertake the “wet-lab” analysis of fission yeast.
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