nanoparticles; encapsulation; toxicity; cellular uptake; pharmacokinetics; biodistribution; implant
Wiedmer Linda, Ducray Angélique D., Frenz Martin, Stoffel Michael H., Widmer Hans-Rudolf, Mevissen Meike (2019), Silica nanoparticle-exposure during neuronal differentiation modulates dopaminergic and cholinergic phenotypes in SH-SY5Y cells, in Journal of Nanobiotechnology
, 17(1), 46-46.
Ducray Angélique D, Felser Andrea, Zielinski Jana, Bittner Aniela, Bürgi Julia V, Nuoffer Jean-Marc, Frenz Martin, Mevissen Meike (2017), Effects of silica nanoparticle exposure on mitochondrial function during neuronal differentiation., in Journal of nanobiotechnology
, 15(1), 49-49.
Ducray Angélique D, Stojiljkovic Ana, Möller Anja, Stoffel Michael H, Widmer Hans-Rudolf, Frenz Martin, Mevissen Meike (2017), Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models., in Nanomedicine : nanotechnology, biology, and medicine
, 13(3), 1195-1204.
Zielinski Jana, Möller Anja-Maria, Frenz Martin, Mevissen Meike (2016), Evaluation of endocytosis of silica particles used in biodegradable implants in the brain., in Nanomedicine : nanotechnology, biology, and medicine
, 12(6), 1603-13.
Möller Anja Maria, Mevissen Meike, Frenz Martin (2015), Nanoparticles for laser tissue soldering in the brain - chances and risks. An interdisciplinary research project, in Bulletin VSH-AEU
, 41(1/2), 80-83.
Koch Franziska, Möller Anja Maria, Frenz Martin, Pieles Uwe, Kuehni-Boghenbor Kathrin, Mevissen Meike (2013), An in vitro toxicity evaluation of gold-, PLLA- and PCL-coated silica nanoparticles in neuronal cells for nanoparticle-assisted laser-tissue soldering, in Toxicology in Vitro
, 28(5), 990-998.
Schönbächler Andrea, Gleied Olfa, Huwyler Jörg, Frenz Martin, Pieles Uwe (2013), Indocyanine green loaded biocompatible nanoparticles: Stabilization of indocyanine green (ICG) using biocompatible silica-poly(ε-caprolactone) grafted nanocomposites, in Journal of Photochemistry and Photobiology A: Chemistry
, 261, 12-19.
Bogni Serge, Schöni Daniel, Constantinescu Mihai, Wirth Amina, Vajtai Istvan, Bregy Amadé, Raabe Andreas, Pieles Uwe, Frenz Martin, Reinert Michael (2012), Tissue fusion: a new opportunity for sutureless bypass surgery, in Tetsuya TsukaharaLuca RegliDaniel HänggiBernd TurowskiHans-Jakob Steiger (ed.), Springer, Wien, New York, 45-52.
Schönbächler Andrea, Andereggen Lukas, Möller Anja, Marti Dominik, Guldimann Claudia, Widmer Hans-Rudolf, Mevisen Meike, Frenz Martin, Reinert Michael, Polycapsulated Silica Core Nanoparticles for Laser Tissue Soldering, in J Neurol Surg A Cent Eur Neurosurg
Nanotechnology is an enabling technology with an enormous impact on many of the currently emerging medical applications such as diagnosis, therapy and prevention of human disease and disorders. Nanotechnology is foreseen to change health care in a fundamental way. One of such application is laser tissue soldering, a kind of tissue fusion which allows to tightly seal surgical wounds and in particular vascular lesions. It is based on a heat induced denaturation process of proteins like bovine serum albumin (BSA), providing the necessary acute tissue strength. This novel tissue fusion technique is perceived as a minimally invasive alternative to the classical use of suture or stitches to close lacerations, which is a powerful perspective in many open and endoscopic surgical applications. It provides essential advantages over traditional suturing including speed, immediate liquid tightness, reduced tissue trauma and faster healing thus reducing the exposure of the patients. This novel laser assisted anastomoses technique will open doors for new avenues of surgical applications especially in neurosurgery where operation time is one crucial parameter. As one major application tissue soldering will be used for cerebral bypass surgery as well as cranial closure techniques. Laser tissue soldering involves the combination of near-infrared radiation, which deeply penetrates tissue, with a biodegradable scaffold in which an exogenous chromophore as heat transducer is embedded e.g. gold nanoparticles (10-80 nm in diameter) or core shell silica nanoparticles (30 -100 nm) containing an encapsulated, ICG dye. The chromophore or gold nanoparticles selectively and locally convert the laser radiation into heat. Over time during the healing process, the biodegradable scaffold will break down releasing the embedded nanoparticles into the surrounding tissue and probably also into the blood stream. The main goal of the project therefore is to determine the nanoparticle biodistribution and their toxicokinetic properties in cell cultures, tissue slices and in vivo. Hereto, we will first study the influence of laser irradiation on the stability and on the physicochemical properties of the nanoparticles since they determine the amount and the pathway of the cellular uptake of the nanoparticles. In a second step we will analyze the mobility and biodistribution of the nanoparticle in brain tissue in vivo i.e. the take-up by the surrounding tissue, the blood stream, and different organs such as liver, spleen, thymus and cervical lymph nodes on a cellular level. In order to track the nanoparticles two approaches will be followed.1. Use of highly sophisticated methods e.g. two photon microscopy, fluorescence correlation spectroscopy, confocal microscopy, AFM and transmission electron microscopy (TEM) to fully characterize the nano-particals prior and after laser irradiation and to analyze histological sections.2. Core shell nanoparticles containing a 14C radiotracer and/or sensitive fluorochrome or a spin label, but exhibiting the same surface properties and size distribution like the ICG doped particles will be monitored by scintillation counting, fluorescences or ESR techniques. Together with the above mentioned investigations, follow-up in-vivo experiments will allow evaluating possible health risks and will enable public health officials in Switzerland and elsewhere to better use current results in making decisions about nanoparticles regulations.The project involves partners from five different Institutes having complementary expertise and establishes a close interdisciplinary research cooperation. Following questions will be addressed: Do nanoparticle change their properties under laser irradiation and how does this affect their interaction with biological tissue? How do the nanoparticles distribute in the adjacent tissue, organs, blood stream or cells after degradation of the scaffold and can any cellular inflammatory responses or adverse effects on the surrounding tissue or organs be detected?Within the three years period of the envisaged project, the following discoveries are expected to be delivered:1. Basic information on particle transport mechanisms in extracellular spaces 2. Mobility and biodistribution of nanoparticles after therapeutic intervention 3. Toxicity of the nanoparticles used for laser assisted tissue soldering.