Project

Back to overview

Synthetic Biology: NANOCELL (09-EuroSYNBIO-FP-012)

English title Synthetic Biology: NANOCELL (09-EuroSYNBIO-FP-012)
Applicant Fotiadis Dimitrios
Number 131038
Funding scheme Project funding (special)
Research institution Institut für Biochemie und Molekulare Medizin Universität Bern
Institution of higher education University of Berne - BE
Main discipline Molecular Biology
Start/End 01.03.2010 - 31.01.2014
Approved amount 397'429.00
Show all

Keywords (6)

nanocell; Synthetic biology; membrane protein; ATP synthase; transport protein; nanomachine

Lay Summary (English)

Lead
Lay summary
The mission of NANOCELL is to engineer biomimetic molecular machineries of the cell as building blocks that can be robustly and flexibly assembled to synthetic vesicles with controllable functionality not found in nature. To approach this goal, we will take nature's cellular machineries apart and explore their potential to reconstitute them in new ways. The synthetic 'NANOCELL' resembling a molecular factory is one strong vision that drives this collaborative research project (CRP).The CRP will master the control of the following biomolecular machines developed by nature: (i) F1Fo ATP synthases, (ii) ATP driven nucleic acid translocating machines, (iii) F1Fo ATP synthase based nanopropellers, (iv) proton-driven drug, solute and peptide transporters, and (v) spectrally tuned light-driven proton pumps. Most of these machines have in common that either their structure and mechanism and/or their function have been characterized to unprecedented accuracy very recently, which bears the chance to move on now to this engineering approach.In the biological cell, the function of these machines is linked either to proton gradients or/and the energy-transporting carrier ATP. From a synthetic biology approach we will reconstitute these machines into stable synthetic (i.e., blockpolymers, synthetic lipids) vesicles. Proton gradients that either power biomolecular machines directly, or ATP synthesis, will be generated by the spectrally tunable light driven proton pumps bacterio- and proteorhodopsins.Within this CRP consisting of eight European Research laboratories, the Fotiadis group will focus during the first three years on the modification, engineering and characterization of bacterial proton-driven drug/solute/peptide transporters and spectrally tuned light-driven proton pumps.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Expression, purification and low-resolution structure of human vitamin C transporter SVCT1 (SLC23A1).
Boggavarapu Rajendra, Jeckelmann Jean-Marc, Harder Daniel, Schneider Philipp, Ucurum Zöhre, Hediger Matthias, Fotiadis Dimitrios (2013), Expression, purification and low-resolution structure of human vitamin C transporter SVCT1 (SLC23A1)., in PloS one, 8(10), 76427-76427.
Peptide transporter DtpA has two alternate conformations, one of which is promoted by inhibitor binding.
Bippes Christian A, Ge Lin, Meury Marcel, Harder Daniel, Ucurum Zöhre, Daniel Hannelore, Fotiadis Dimitrios, Müller Daniel J (2013), Peptide transporter DtpA has two alternate conformations, one of which is promoted by inhibitor binding., in Proceedings of the National Academy of Sciences of the United States of America, 110(42), 3978-86.
Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay.
Harder Daniel, Fotiadis Dimitrios (2012), Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay., in Nature protocols, 7(9), 1569-78.
Structure and function of the glucose PTS transporter from Escherichia coli.
Jeckelmann Jean-Marc, Harder Daniel, Mari Stefania A, Meury Marcel, Ucurum Zöhre, Müller Daniel J, Erni Bernhard, Fotiadis Dimitrios (2011), Structure and function of the glucose PTS transporter from Escherichia coli., in Journal of structural biology, 176(3), 395-403.
Structure determination of channel and transport proteins by high-resolution microscopy techniques.
Meury Marcel, Harder Daniel, Ucurum Zöhre, Boggavarapu Rajendra, Jeckelmann Jean-Marc, Fotiadis Dimitrios (2011), Structure determination of channel and transport proteins by high-resolution microscopy techniques., in Biological chemistry, 392(1-2), 143-50.

Associated projects

Number Title Start Funding scheme
139231 Advancement of functional genomics research at the University of Bern by extension of LC-MS platform 01.07.2012 R'EQUIP

-