arabidopsis; chromatin; development; vernalization; transcription; Polycomb; histone; gene expression
Müller Marlen, Patrignani Andrea, Rehrauer Hubert, Gruissem Wilhelm, Hennig Lars (2012), Evaluation of alternative RNA labeling protocols for transcript profiling with Arabidopsis AGRONOMICS1 tiling arrays., in Plant methods
, 8(1), 18-18.
Houba-Hérin Nicole, Hennig Lars, Köhler Claudia, Gaudin Valérie (2012), A fruitful chromatin harvest: meeting summary of the Second European Workshop on Plant Chromatin 2011 in Versailles, France., in Epigenetics : official journal of the DNA Methylation Society
, 7(3), 307-11.
Hennig Lars (2012), Plant gene regulation in response to abiotic stress., in Biochimica et biophysica acta
, 1819(2), 85-85.
Bischof S, Baerenfaller K, Wildhaber T, Troesch R, Vidi PA, Roschitzki B, Hirsch-Hoffmann M, Hennig L, Kessler F, Gruissem W, Baginsky S (2011), Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of N-Acetylated Plastid Precursor Proteins, in PLANT CELL
, 23(11), 3911-3928.
Ohno Yusuke, Narangajavana Jarunya, Yamamoto Akiko, Hattori Tsukaho, Kagaya Yasuaki, Paszkowski Jerzy, Gruissem Wilhelm, Hennig Lars, Takeda Shin (2011), Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance., in Genetics
, 189(1), 83-95.
Gutzat Ruben, Borghi Lorenzo, Fütterer Johannes, Bischof Sylvain, Laizet Yec'han, Hennig Lars, Feil Regina, Lunn John, Gruissem Wilhelm (2011), RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development., in Development (Cambridge, England)
, 138(14), 2977-86.
Exner Vivien, Alexandre Cristina, Rosenfeldt Gesa, Alfarano Pietro, Nater Mena, Caflisch Amedeo, Gruissem Wilhelm, Batschauer Alfred, Hennig Lars (2010), A gain-of-function mutation of Arabidopsis cryptochrome1 promotes flowering., in Plant physiology
, 154(4), 1633-45.
Möller-Steinbach Yvonne, Alexandre Cristina, Hennig Lars (2010), Flowering time control., in Methods in molecular biology (Clifton, N.J.)
, 655, 229-37.
Streitner Corinna, Hennig Lars, Korneli Christin, Staiger Dorothee (2010), Global transcript profiling of transgenic plants constitutively overexpressing the RNA-binding protein AtGRP7., in BMC plant biology
, 10, 221-221.
Hennig Lars (2010), Growth protocols for model plants in developmental biology., in Methods in molecular biology (Clifton, N.J.)
, 655, 1-10.
Weinhofer Isabelle, Hehenberger Elisabeth, Roszak Pawel, Hennig Lars, Köhler Claudia (2010), H3K27me3 profiling of the endosperm implies exclusion of polycomb group protein targeting by DNA methylation., in PLoS genetics
, 6(10), e1001152-e1001152.
Köhler Claudia, Hennig Lars (2010), Regulation of cell identity by plant Polycomb and trithorax group proteins., in Current opinion in genetics & development
, 20(5), 541-7.
Borghi Lorenzo, Gutzat Ruben, Fütterer Johannes, Laizet Yec'han, Hennig Lars, Gruissem Wilhelm (2010), Arabidopsis RETINOBLASTOMA-RELATED is required for stem cell maintenance, cell differentiation, and lateral organ production., in The Plant cell
, 22(6), 1792-811.
Established gene expression profiles need to be faithfully transmitted over many cell generations. In multicellular organisms, this function is carried out by chromatin modifying machineries that maintain established transcriptional states. Although the underlying molecular mechanisms are still only poorly understood, during recent years it became evident that Polycomb group (PcG) proteins play an essential role in this process. PcG proteins are evolutionary conserved proteins that assemble into multimeric complexes with enzymatic activity. The Polycomb Repressive Complex 2 (PRC2) establishes trimethylation marks on histone H3 lysine 27 (H3K27me3). In insects and mammals H3K27me3 is bound by the PRC1 complex that subsequently ubiquitylates histone H2A leading to transcriptional repression. Components of PRC1 are not very well conserved in plants, and the mechanisms of PcG mediated gene repression in plants are unknown. The LIKE HETEROCHROMATIN PROTEIN1 (LHP1) binds to H3K27me3-rich regions; however, it remains enigmatic how LHP1 mediates transcriptional repression. Here, I propose to uncover molecular mechanisms of gene regulation by detailed investigations of PcG protein functions in plants. Specifically, the aim of this research proposal is to elucidate by which mechanisms PcG proteins are directed to their target loci and how different PcG complexes distinguish their target genes. Furthermore, we want to reveal mechanisms of PcG-mediated transcriptional repression in plants and to functionally connect chromatin modifications with changes of the transcriptional status of a gene. Therefore, we plan to identify histone marks that are coregulated with H3K27me3. The precise regulation of PcG target gene expression is crucial to prevent cancer development in mammals as well as to maintain stem cell fate; therefore, the results of our investigations will likely be of high interest for a broad scientific and medical community.