Project

Back to overview

Innate immune functions of immature neutrophils (band forms)

English title Innate immune functions of immature neutrophils (band forms)
Applicant Drifte Geneviève
Number 128875
Funding scheme MD-PhD fellowships (funded by SNSF)
Research institution Dépt Microbiologie et Médecine Moléculaire Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Clinical Immunology and Immunopathology
Start/End 01.07.2009 - 31.05.2012
Approved amount 175'000.00
Show all

All Disciplines (2)

Discipline
Clinical Immunology and Immunopathology
Immunology, Immunopathology

Keywords (4)

sepsis; innate immunity; left shift; immature neutrophils

Lay Summary (English)

Lead
Lay summary
Polymorphonuclear neutrophils, or granulocytes, are essential effector cells of the innate immune system against bacterial infections. Their role in sepsis has been long established as the primary phagocyte to clear the infectious process. In the early phase of sepsis, one observes a massive recruitment of immature neutrophils from the bone marrow into peripheral blood, the so-called "band forms" or "left shift cells". Despite the daily clinical use of neutrophil band forms count in the care of septic patients and their abundance in septic blood, no information exists on the fate of these cells, nor on their capacity to mount an efficient innate immune response. It is the goal of this proposal to study the fate and the innate immune functions of immature neutrophils obtained in patients with early septic shock. This is rendered possible by a recently identified differential density of these cells in a Ficoll® gradient, which allows to separate septic blood immature from mature neutrophils. The following functions will be studied ex vivo in mature vs. immature neutrophils from a series of patients with severe sepsis and septic shock: (1) surface expression of receptors of the innate immunity; (2) production of inflammatory mediators and reactive oxygen species in response to bacterial agonists; (3) chemotaxis; (4)phagocytosis of Gram-positive and Gram-negative bacteria; and (5) ex vivo viability (life span) and resistance to apoptosis. Importantly, we have developed and mastered all in vitro assays and cell separation techniques necessary to address and answer these important questions. This project will undoubtedly shed light on the fate and function of a prominent leukocyte population circulating in patients with severe bacterial infections and sepsis.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

-