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Biomedical applications of femtosecond lasers: Gold colloids and phospholipid membranes.

Applicant Ricka Jaroslav
Number 126957
Funding scheme Project funding (Div. I-III)
Research institution Institut für angewandte Physik Universität Bern
Institution of higher education University of Berne - BE
Main discipline Other disciplines of Physics
Start/End 01.10.2009 - 30.09.2010
Approved amount 68'979.00
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All Disciplines (2)

Discipline
Other disciplines of Physics
Biophysics

Keywords (7)

biophotonics; femtosecond pulses; gold nanoparticles; bilayers; gold nano-particles; phospholipid membranes; multiphoton luminescence

Lay Summary (English)

Lead
Lay summary
The aim of the project is to contribute to the understanding of the interaction of femtosecond NIR pulses with their biological primary target, namely with the cell membrane. Thereby, gold nano-particles will be involved in a two fold role: i) we shall use them as luminescent markers to track the evolution of the state of the membrane after the laser treatment. ii) We shall use them as highly absorbing sensitizers to induce localized membrane changes in an efficient way. The task of the present project period is to expand our knowledge about gold nano-particles response to high intensity laser pulses. In particular, we would like to contribute to the understanding of the origin of their multi-photon excited luminescence.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Name Institute

Associated projects

Number Title Start Funding scheme
113284 Particle tracking in three-dimensions using multiphoton luminescence in gold nanoparticles 01.10.2006 Project funding (Div. I-III)

Abstract

Light can be used as an information carrier for characterization or imaging as well as a tool for manipulating or processing of bio-matter. Ultrashort NIR pulses offer certain unique advantages in both areas of application. At low intensities, near infra¬red light interacts only little with biological matter. However, if the light energy is concentrated into a period of time as short as 100 femtoseconds, in a focal spot as small as a fraction of one wavelength, then non-linear multiphoton light-matter interactions become effective. These non-linear effects are confined in a nano-sized region in the focus of the beam. Thus, one can dissect a selected organelle, without damaging the cell otherwise. The same property of the ultrashort pulses, but at a much lower light level, makes the femtosecond NIR laser an excellent light source for high resolution imaging in living cells. The topic of the present project is a combination of imaging and fundamental aspects of nanosurgery. The primary targets of nanosurgical ablation of a biological cell by femtosecond lasers are the cell membranes or various intracellular membranes. This project’s aim is to elucidate the processes that occur in the phospholipid membrane upon the interaction with the laser pulses. Thereby, gold nano-particles will be involved in a two fold role: i) we shall use them as luminescent markers to track the evolution of the state of the membrane after the laser treatement. ii) we shall use them as highly absorbing sensitizers to induce localized membrane changes in an efficient way.
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