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Tracking of Phosphoinositide Pools - A Key Signalling Component in Cancer and Inflammation (EMRC/ECORES/08-EUROMEMBRANE)

English title Tracking of Phosphoinositide Pools - A Key Signalling Component in Cancer and Inflammation (EMRC/ECORES/08-EUROMEMBRANE)
Applicant Wymann Matthias
Number 126143
Funding scheme Project funding (special)
Research institution Institut für Biochemie und Genetik Universität Basel
Institution of higher education University of Basel - BS
Main discipline Molecular Biology
Start/End 01.07.2009 - 30.06.2012
Approved amount 613'872.00
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All Disciplines (3)

Discipline
Molecular Biology
Biochemistry
Genetics

Keywords (12)

lipid dynamics; phospohinositides; advanced imaging techniques; chemical biology; PI3K; chemistry; Lipid Signaling; Imaging; Molecular Biology; Cancer; Inflammation; Phosphoinositides

Lay Summary (English)

Lead
Lay summary
We propose a unique consortium (TraPPs) between leading European groups in phosphoinositide (PI) signalling, lipid probe chemists and advanced microscopy centers. PI signalling plays a central role in membrane dynamics modulating cell polarity, vesicular trafficking, migration, growth, proliferation, differentiation, and more. TraPPs will provide a quantitative, dynamic and refined view of phosphoinositide flux, required lipid modifying enzymes, e.g. PI3Ks, lipases, and lipid phosphatases. To this end novel activatable PPI probes will be synthesized and loaded into cells; lipid-modifying enzymes will be targeted dynamically to distinct cellular locations; lipid-interacting proteins shall be manipulated to display their free or lipid-bound state; and the activity state of phospholipid-modifying enzymes will be quantified by imaging the formation of enzyme-substrate complexes in living cells. We will use well-defined cellular, genetic fly and mouse models validating the uncovered molecular mechanisms in terms of cell migration, cell-cell contact changes, vesicular trafficking and epithelial-mesenchymal transitions, which together will elucidate cellular signaling processes relevant to cancer and inflammation. We expect a quantum leap in knowledge to the subject of PI signaling since to our knowledge this massive accumulation of expertise and close collaboration has never been realized for this field in Europe before.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Associated projects

Number Title Start Funding scheme
133860 High Content and High Throughput Microscopy to Decipher Signaling Networks 01.12.2010 R'EQUIP
138302 Chemische Induktion der intrazellulären Signalübertragung 01.01.2012 Project funding (Div. I-III)
127574 Regulation von PI3K in Progressiven Krankheiten 01.10.2009 Project funding (Div. I-III)
139260 Rapid, multi-mode, live imaging and photo-manipulation of protein and activation dynamics at the plasma membrane 01.01.2012 R'EQUIP
153211 Targeting PI3K Interactions in Disease 01.01.2015 Project funding (Div. I-III)
143699 Kovalente Tag Chemie - Intrazelluläre Kontrolle von Receptoren und Signalen 01.01.2013 Project funding (Div. I-III)

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