Caulobacter crescentus; Cell cycle; DNA replication; Epigenetic; Systems biology; DNA methylation; DnaA; Caulobacter
Fernandez-Fernandez Carmen, Gonzalez Diego, Collier Justine (2011), Regulation of the Activity of the Dual-Function DnaA Protein in Caulobacter crescentus., in PloS one
, 6(10), 0-0.
Collier J (2010), A new factor stimulating peptidoglycan hydrolysis to separate daughter cells in Caulobacter crescentus, in MOLECULAR MICROBIOLOGY
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Collier Justine (2009), Epigenetic regulation of the bacterial cell cycle., in Current opinion in microbiology
, 12(6), 722-9.
Collier J, Shapiro L (2009), Feedback Control of DnaA-Mediated Replication Initiation by Replisome-Associated HdaA Protein in Caulobacter, in JOURNAL OF BACTERIOLOGY
, 191(18), 5706-5716.
Collier Justine, Regulation of chromosomal replication in Caulobacter crescentus, in Plasmid
To proliferate, bacteria must grow, duplicate and segregate their genome, and divide to separate the cell into two. We wish to understand how chromosome replication is coordinated with cell division and development, so that DNA replication occurs during the appropriate period of the cell cycle in bacteria. The bacterium Caulobacter crescentus exemplifies this fundamental control problem, because its cellular differentiation is an obligate part of its cell cycle, it replicates its DNA once-and-only-once per cell cycle, and it is easy to obtain a population of cells that are at the same stage of the cell cycle, to study the temporal regulation of the cell cycle. We propose to characterize the multiple regulatory pathways that control the timing of the initiation of DNA replication and the segregation of the chromosome in Caulobacter cells. We expect that epigenetic mechanisms of regulation will play key roles in these processes. Specifically, we will:-Determine how the initiator of DNA replication, DnaA, is temporally and spatially regulated during the cell cycle-Characterize the roles of two dnaA-related genes found in the Caulobacter genome-Identify the various roles of DNA methylation in the control of the timing of gene transcription, DNA replication and chromosome segregation-Determine the mechanisms by which transcription is regulated by DNA methylation-Identify and characterize checkpoint mechanisms that control DNA replication and coordinate it with cell division and DNA repair systems.Each regulatory pathway will be characterized independently, but our ultimate goal is to combine all the results to analyze the cell cycle control circuit an integrated system.