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Regulation of DNA replication and coordination of cell cycle events in Caulobacter crescentus

Gesuchsteller/in Collier Close Justine
Nummer 122541
Förderungsinstrument Projektförderung (Abt. I-III)
Forschungseinrichtung Département d'Ecologie et d'Evolution Faculté de Biologie et de Médecine Université de Lausanne
Hochschule Universität Lausanne - LA
Hauptdisziplin Experimentelle Mikrobiologie
Beginn/Ende 01.12.2008 - 30.09.2012
Bewilligter Betrag 299'693.00
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Alle Disziplinen (2)

Disziplin
Experimentelle Mikrobiologie
Zellbiologie, Zytologie

Keywords (8)

Caulobacter crescentus; Cell cycle; DNA replication; Epigenetic; Systems biology; DNA methylation; DnaA; Caulobacter

Lay Summary (Englisch)

Lead
Lay summary
To proliferate, bacteria must grow, duplicate and segregate their genome, and divide to separate the cell into two. We wish to understand how chromosome replication is coordinated with cell division and development, so that DNA replication occurs during the appropriate period of the cell cycle in bacteria. The bacterium Caulobacter crescentus exemplifies this fundamental control problem, because its cellular differentiation is an obligate part of its cell cycle, it replicates its DNA once-and-only-once per cell cycle, and it is easy to obtain a population of cells that are at the same stage of the cell cycle, to study the temporal regulation of the cell cycle. We propose to characterize the multiple regulatory pathways that control the timing of the initiation of DNA replication and the segregation of the chromosome in Caulobacter cells. We expect that epigenetic mechanisms of regulation will play key roles in these processes. Specifically, we will:-Determine how the initiator of DNA replication, DnaA, is temporally and spatially regulated during the cell cycle-Characterize the roles of two dnaA-related genes found in the Caulobacter genome-Identify the various roles of DNA methylation in the control of the timing of gene transcription, DNA replication and chromosome segregation-Determine the mechanisms by which transcription is regulated by DNA methylation-Identify and characterize checkpoint mechanisms that control DNA replication and coordinate it with cell division.Each regulatory pathway will be characterized independently, but our ultimate goal is to combine all the results to analyze the cell cycle control circuit as an integrated system.
Direktlink auf Lay Summary Letzte Aktualisierung: 21.02.2013

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Mitarbeitende

Publikationen

Publikation
Regulation of the Activity of the Dual-Function DnaA Protein in Caulobacter crescentus.
Fernandez-Fernandez Carmen, Gonzalez Diego, Collier Justine (2011), Regulation of the Activity of the Dual-Function DnaA Protein in Caulobacter crescentus., in PloS one, 6(10), 0-0.
A new factor stimulating peptidoglycan hydrolysis to separate daughter cells in Caulobacter crescentus
Collier J (2010), A new factor stimulating peptidoglycan hydrolysis to separate daughter cells in Caulobacter crescentus, in MOLECULAR MICROBIOLOGY, 77(1), 11-14.
Epigenetic regulation of the bacterial cell cycle.
Collier Justine (2009), Epigenetic regulation of the bacterial cell cycle., in Current opinion in microbiology, 12(6), 722-9.
Feedback Control of DnaA-Mediated Replication Initiation by Replisome-Associated HdaA Protein in Caulobacter
Collier J, Shapiro L (2009), Feedback Control of DnaA-Mediated Replication Initiation by Replisome-Associated HdaA Protein in Caulobacter, in JOURNAL OF BACTERIOLOGY, 191(18), 5706-5716.
Regulation of chromosomal replication in Caulobacter crescentus
Collier Justine, Regulation of chromosomal replication in Caulobacter crescentus, in Plasmid.

Wissenschaftliche Veranstaltungen

Aktiver Beitrag

Titel Art des Beitrags Titel des Artikels oder Beitrages Datum Ort Beteiligte Personen
Society for General Microbiology Autumn Conference 10.09.2012 Warwick, UK
14th International Symposium on Microbial Ecology 01.09.2012 Copenhagen, Denmark
4th ASM conference on Prokaryotic Cell Biology and Development 05.05.2012 Montreal, Canada
Caulobacter meeting 01.05.2012 Montreal, Canada
FEMS meeting 01.07.2011 Geneva, Switzerland
ESF "Bacnet" meeting 01.09.2010 San Feliu de Guixol
EMBO "Chromosome organization and replication" meeting 01.06.2010 Freiburg, GER
Swiss Society of Microbiology Meeting 01.06.2010 Zurich
Colloque Jacques Monod 03.05.2010 Paris, France
Caulobacter Meeting 01.06.2009 Boston, USA
Swiss Society of Microbiology Meeting 01.06.2009 Lausanne


Selber organisiert

Titel Datum Ort
Symposium "Bacteria: from genome to life" 03.11.2008 Lausanne

Kommunikation mit der Öffentlichkeit

Kommunikation Titel Medien Ort Jahr
Referate/Veranstaltungen/Ausstellungen Journées de Gymnasiens Westschweiz
Referate/Veranstaltungen/Ausstellungen Journées Osez Tous les Métiers Westschweiz
Referate/Veranstaltungen/Ausstellungen Mystères de l'UNIL Westschweiz

Verbundene Projekte

Nummer Titel Start Förderungsinstrument
140758 Coordination between chromosomal replication and other events of the Caulobacter crescentus cell cycle 01.10.2012 Projektförderung (Abt. I-III)

Abstract

To proliferate, bacteria must grow, duplicate and segregate their genome, and divide to separate the cell into two. We wish to understand how chromosome replication is coordinated with cell division and development, so that DNA replication occurs during the appropriate period of the cell cycle in bacteria. The bacterium Caulobacter crescentus exemplifies this fundamental control problem, because its cellular differentiation is an obligate part of its cell cycle, it replicates its DNA once-and-only-once per cell cycle, and it is easy to obtain a population of cells that are at the same stage of the cell cycle, to study the temporal regulation of the cell cycle. We propose to characterize the multiple regulatory pathways that control the timing of the initiation of DNA replication and the segregation of the chromosome in Caulobacter cells. We expect that epigenetic mechanisms of regulation will play key roles in these processes. Specifically, we will:-Determine how the initiator of DNA replication, DnaA, is temporally and spatially regulated during the cell cycle-Characterize the roles of two dnaA-related genes found in the Caulobacter genome-Identify the various roles of DNA methylation in the control of the timing of gene transcription, DNA replication and chromosome segregation-Determine the mechanisms by which transcription is regulated by DNA methylation-Identify and characterize checkpoint mechanisms that control DNA replication and coordinate it with cell division and DNA repair systems.Each regulatory pathway will be characterized independently, but our ultimate goal is to combine all the results to analyze the cell cycle control circuit an integrated system.
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