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Microscope à fluorescence pour détecter des protéines fluorescentes dans des cellules microscopiques.

Titel Englisch Fluorescence microscope system optimized to detect fluorescent proteins in small microbial cells.
Gesuchsteller/in Collier Close Justine
Nummer 121391
Förderungsinstrument R'EQUIP
Forschungseinrichtung Département d'Ecologie et d'Evolution Faculté de Biologie et de Médecine Université de Lausanne
Hochschule Universität Lausanne - LA
Hauptdisziplin Experimentelle Mikrobiologie
Beginn/Ende 01.09.2008 - 31.08.2009
Bewilligter Betrag 106'457.00
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Alle Disziplinen (2)

Disziplin
Experimentelle Mikrobiologie
Zellbiologie, Zytologie

Keywords (8)

Microbiology; Microbial cell biology; Microbial ecology; Microbial pathogenicity; cell biology; ecology; pathogenicity; protein localisation

Lay Summary (Englisch)

Lead
Lay summary
For more than a century, cell biology has been practiced on eukaryotic cells, because the study of bacterial cell biology was limited by the lack of good cytological techniques to study very small cells. As a consequence, bacteria were long dismissed as featureless, disorganized sacks. Thanks to technological advances that have facilitated the resolution of structural details within bacterial cells, recent work has revealed that bacteria also exhibit a high degree of intracellular organization. Bacteria have evolved mechanisms to target protein complexes and DNA to specific positions within the cells, to actively control cell cycle progression, morphological differentiation, and adaptation to the environment.An excellent widefield microscope system is an indispensable tool for modern bacterial cell biology research. The research we propose to do ourselves with this microscope system ranges from studying the spatial and the temporal regulation of the bacterial cell cycle using Caulobacter crescentus as a model system, to understanding the cellular decisions which engage a bacterial cell in conjugation, utilizing reporters systems for bioavailability studies, studying the interaction od the pathogenic bacteria Staphylococcus aureus with the Caenorhabditis elegans model host, and identifying the resistance strategies elaborated by the pathogenic yeast Candida albicans.
Direktlink auf Lay Summary Letzte Aktualisierung: 21.02.2013

Verantw. Gesuchsteller/in und weitere Gesuchstellende

Verbundene Projekte

Nummer Titel Start Förderungsinstrument
140758 Coordination between chromosomal replication and other events of the Caulobacter crescentus cell cycle 01.10.2012 Projektförderung (Abt. I-III)

Abstract

For more than a century, cell biology has been practiced on eukaryotic cells, because the study of bacterial cell biology was limited by the lack of good cytological techniques to study very small cells. As a consequence, bacteria were long dismissed as featureless, disorganized sacks. Thanks to technological advances that have facilitated the resolution of structural details within bacterial cells, recent work has revealed that bacteria also exhibit a high degree of intracellular organization. Like eukaryotes, prokaryotes have evolved mechanisms to target protein complexes and DNA to specific positions within the cells, to actively control cell cycle progression, morphological differentiation, and adaptation to the environment or to a host.An excellent widefield upright microscope system is an indispensable tool for modern bacterial cell biology research. To detect poor quantities of fluorescent proteins at specific subcellular locations within tiny bacterial cells, the microscope must be equipped with high-performance objectives and a high-end CCD camera. Multiple filters are also necessary to look at the dynamic localization of different fluorescent proteins in the same cells. The automated XYZ stage mounted on this microscope would be very advantageous to take several fields of cells during a single time-lapse experiment, and to do Z-stacks recordings to do deconvolution. We see various important research lines that can highly profit from this tool, and since no such an advanced system is accessible on a regional scale, we believe the investment is definitively justified.The research we propose to do ourselves with this microscope system ranges from studying the spatial and the temporal regulation of the bacterial cell cycle using Caulobacter crescentus as a model system, to understanding the cellular decisions which engage a bacterial cell in conjugation, utilizing reporter systems for bioavailability studies, studying the interaction of the pathogenic bacteria Staphylococcus aureus with the Caenorhabditis elegans model host, and identifying the resistance strategies elaborated by the pathogenic yeast Candida albicans. However, we foresee that many more laboratories working on small organisms, like bacteria, will be interested in studying the localization, structure, and dynamics of macromolecules in living cells.
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