central pattern generator; rhythm generation; oscillations; plasticity; Neural network; spinal cord; cortex; regeneration
Heidemann M, Streit J, Tscherter A (2014), Functional regeneration of intraspinal connections in a new in vitro model., in Neuroscience
, 262, 40-52.
Hofer Sandra, Magloire Vincent, Streit Juerg, Leib Stephen L. (2012), Grafted Neuronal Precursor Cells Differentiate and Integrate in Injured Hippocampus in Experimental Pneumococcal Meningitis, in STEM CELLS
, 30(6), 1206-1215.
Czarnecki Antonny, Tscherter Anne, Streit Jürg (2012), Network activity and spike discharge oscillations in cortical slice cultures from neonatal rat., in The European journal of neuroscience
, 35(3), 375-88.
Magloire V., Czarnecki A., Anwander H., Streit J. (2011), beta-POMPILIDOTOXIN MODULATES SPONTANEOUS ACTIVITY AND PERSISTENT SODIUM CURRENTS IN SPINAL NETWORKS, in NEUROSCIENCE
, 172, 129-138.
Magloire Vincent, Streit Juerg (2009), Intrinsic activity and positive feedback in motor circuits in organotypic spinal cord slice cultures, in EUROPEAN JOURNAL OF NEUROSCIENCE
, 30(8), 1487-1497.
Czarnecki Antonny, Magloire Vincent, Streit Juerg (2009), Modulation of Intrinsic Spiking in Spinal Cord Neurons, in JOURNAL OF NEUROPHYSIOLOGY
, 102(4), 2441-2452.
Czarnecki Antonny, Magloire Vincent, Streit Juerg (2008), Local oscillations of spiking activity in organotypic spinal cord slice cultures, in EUROPEAN JOURNAL OF NEUROSCIENCE
, 27(8), 2076-2088.
Practically all functions of the central nervous system emerge on the level of networks of neurons. In the mammalian brain such networks are interacting in a complex and distributed way, making their analysis difficult. Emerging properties of defined small networks are therefore better investigated in simplified systems like cell cultures. A key function of these networks is the generation of rhythmic patterns of activity. In the spinal cord such activity patterns are used to drive repetitive movements for locomotion. In the cortex, rhythms are involved in perception and memory processes. Our project is involved in studying the mechanisms of rhythm and pattern generation in neural networks in culture. We are doing this by combining multielectrode array (MEA) recordings to investigate the network level with intracellular patch clamp recordings to investigate the cellular level. At the moment we are interested in the influence of network size and network density on rhythm generation. We will test the hypothesis that a minimal network density is required for the generation of oscillatory activity that is based on repetitive network activation through recurrent excitation. In addition, we will investigate the role of propriospinal connections along the longitudinal axis of the spinal cord for the generation of defined patterns of rhythmic activity and their regeneration potential as a function of age. Rhythm generation is also a prominent feature of cortical networks in organotypic slice cultures from neonatal rats. We will investigate the mechanisms underlying such activity. Furthermore we want to know how rhythm generation is modified by electrical stimulation protocols and by chemical neuromodulators. The major aim of our project is to gain new insights into the mechanisms of phenomena, which emerge on the level of neural networks. We hope that such basic knowledge about rhythm and pattern generation in newly formed networks will help to evaluate how fetal neurons form functional networks when transplanted into the injured brain or spinal cord for therapeutic purposes.