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DEAD-box RNA helicases orchestrate translation initiation in bacteria and eukaryotes

English title DEAD-box RNA helicases orchestrate translation initiation in bacteria and eukaryotes
Applicant Linder Patrick
Number 118309
Funding scheme Project funding (Div. I-III)
Research institution Dépt Microbiologie et Médecine Moléculaire Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Molecular Biology
Start/End 01.10.2007 - 30.09.2010
Approved amount 486'409.00
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Keywords (6)

RNA helicase; DEAD-box; translation initiation; biofilm; saccharomyces cerevisiae; yeast

Lay Summary (English)

Lead
Lay summary
DEAD-box RNA helicases orchestrate translation initiation in bacteria and eukaryotesRNA molecules are long linear polymers that tend naturally to form secondary structures based on partially complementary sequences. These molecules have many functions such as information carrier, adaptor molecules, ribozymes, structural components, or regulatory molecules. In order to be functional, many RNA molecules have to go through dynamic processes of maturation and be available in particular conformations. A large class of proteins, the so-called RNA helicases, help to rearrange RNA secondary structures, RNA-RNA interactions or RNA protein complexes. This dynamic rearrangement of complexes does require the input of energy, in form of NTP hydrolysis.The RNA helicases can be subdivided into different families, based on some conserved sequence motifs. One of these families is the DEAD-box family, named after one of the conserved motifs of amino acids (Asp-Glu-Ala-Asp). Proteins of this family are found in all three kingdoms of life and eukaryotic organisms have a multitude of these proteins. They are required for all process involving RNA molecules, such as transcription, pre-mRNA maturation, ribosome biogenesis, RNA export, translation initiation and RNA degradation. Although the eukaryotic genomes encode many different DEAD-box proteins (25 in bakers yeast, 38 in humans), they are all highly specific and cannot replace each other.The high diversity and specificity of these proteins suggest that they are thoroughly regulated and can act only at the right time and at the right place. Our laboratory has been interested in DEAD-box proteins for many years. Recently our focus turned to the protein Ded1, required for translation initiation in the bakers yeast Saccharomyces cerevisiae and to Sa1885, a DEAD-box protein from Staphylococcus aureus required for biofilm formation. We will use in our work both biochemical and genetic methods to analyse the function of these two proteins and their involvement in gene expression.
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
132638 Acquisition and regulation of virulence factors in Staphylococcus aureus 01.10.2010 Project funding (Div. I-III)
105894 RNA helicases in translation initiation and ribosome biogenesis 01.10.2004 Project funding (Div. I-III)

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