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Flow cytometry opens new avenues for cell-based analyses in biomedical research

English title Flow cytometry opens new avenues for cell-based analyses in biomedical research
Applicant Schneiter Roger
Number 117392
Funding scheme R'EQUIP
Research institution Division de Biochimie Département de Biologie Université de Fribourg
Institution of higher education University of Fribourg - FR
Main discipline Biochemistry
Start/End 01.08.2007 - 31.07.2008
Approved amount 140'208.00
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All Disciplines (2)

Discipline
Biochemistry
Cellular Biology, Cytology

Keywords (6)

flow cytometry; lipid uptake and transport; circadian clock; aging; reactive oxygen species; cell cycle

Lay Summary (English)

Lead
Lay summary
This research proposal requests funding for the acquisition of a flow cytometer equipped with a temperature-controlled autosampler. Such an instrument allows a quantitative readout from a variety of fluorescent cell-based assay systems, which are being used, for example, to analyze cell cycle progression, to monitor apoptosis to quantify lipid transport, and to analyze cell surface expression of marker proteins. In combination with an autosampler, the instrument is suited for high-throughput screens, which have now become extremely successful for gene discovery in organisms for which deletion libraries or siRNA collections are available, such as yeast, C. elegans, T. brucei, and mammalian cells.There is presently no flow cytometer or equivalent instrument available at our campus. The instrument would thus allow us to perform experiments that are currently either not possible, or require much more time and reagents than a corresponding readout based on flow cytometry. The four SNF-funded research groups applying for this grant will be the main users of this instrument, but additional groups have already expressed their interest in using this equipment. The four groups will employ the instrument to study (i) lipid homeostasis in yeast, (ii) the connection between the circadian clock and aging, (iii) the coordination between mitochondrial biogenesis and the cell cycle in the parasitic protozoan, Trypanosoma brucei, and (iv) the role of adipose tissues of different origins in obesity-associated vascular dysfunctions. As is evident from this list of topics, the requested instrument is extremely versatile and thus will serve the needs and will be open to the more than 25 research groups in the Life Sciences that are located on the campus. This flow cytometer would thus well complement more qualitative readouts that are presently typically performed by fluorescent microscopy.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

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