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Potential of FGF2-transduced neural progenitors for generating vascular niches

English title Potential of FGF2-transduced neural progenitors for generating vascular niches
Applicant Kiss Jozsef Zoltan
Number 116613
Funding scheme Project funding (Div. I-III)
Research institution Dépt des Neurosciences Fondamentales Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Neurophysiology and Brain Research
Start/End 01.04.2007 - 31.03.2010
Approved amount 260'000.00
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Keywords (6)

cell migration; vascular niche; VEGF; FGF-2; neural progenitor; ischemic brain lesion

Lay Summary (English)

Lay summary
The capacity for structural self-repair in the postnatal cortex is very limited. This is due in part to the lack of a resident population of neural progenitors responsive to signals derived from the damaged tissue. Therefore supplying a pool of multipotential, proliferating and migrating neural stem/progenitor cells (NPCs) would be of considerable interest. However this has proven difficult to achieve mainly because transplanted NPCs rapidly loose their proliferative and migratory phenotype. To address this issue we recently developed a novel system to over-express the major mitogenic growth factor, FGF2 in NPCs and studied its effects on several cellular functions after transplantation into postnatal brain tissue. We found that FGF2 lentiviral transduction increases the proliferative and migratory potential of NPCs in a complex 3-dimensional structure such as rat cortical slices and after in vivo transplantation. A major observation of these studies is that FGF2 over-expressing NPCs had the distinct tendency to form proliferating clusters around small blood vessels. Most importantly, these clusters seem to provide new neurons in an ischemic environment. The central hypothesis we propose to test in this project is that FGF2 over-expressing NPCs are able to form vascular niches with stem cell function after grafting into the postnatal cortex. We predict that the reciprocal interaction of NPCs with the perivascular microenvironment is the key factor dictating the functional outcome of the niche.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants


Associated projects

Number Title Start Funding scheme
128015 Ectopic niche formation by grafted neural stem/progenitors in the ischemic cerebral cortex 01.01.2010 SCOPES
104059 Molecular control of neural progenitor migration 01.04.2004 Project funding (Div. I-III)