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Selective cell diagnosis and killing by nanoparticle absorption of pulsed laser radiation

English title Selective cell diagnosis and killing by nanoparticle absorption of pulsed laser radiation
Applicant Frenz Martin
Number 116343
Funding scheme Project funding (Div. I-III)
Research institution Institut für angewandte Physik Universität Bern
Institution of higher education University of Berne - BE
Main discipline Other disciplines of Physics
Start/End 01.04.2007 - 30.09.2009
Approved amount 180'447.00
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All Disciplines (2)

Discipline
Other disciplines of Physics
Clinical Pharmacology

Keywords (7)

OPTOACOUSTIC IMAGING; minimally invasive therapy; NANOPARTICLES; SURFACE PLASMON RESONANCE ABSORPTION; GOLD COLLOID; selective cell targeting; photodamage

Lay Summary (English)

Lead
Lay summary
Optoacoustic imaging of biological tissue is a technique undergoing rapid development with great promise for early breast and skin cancer diagnostics. It allows to visualize internal biological structures and function of soft tissue. The image contrast is provided by light absorbing chromophores that are either endogenous, such as oxy- or deoxyhemoglobin, or exogenous e.g. dyes, nanoparticles or quantum dots. Optoacoustics is based on the time-resolved detection of ultrasound waves over the tissue surface which were generated following selective absorption of a short laser pulse. The time delay between laser pulse and detected pressure transient and its amplitude and temporal profile provide information about location, strength and spatial dimension of the acoustic source, which can be reconstructed using suitable reconstruction algorithms. Optoacoustic techniques have already been used to visualize breast cancer: The increased blood content supplying the tumor's aggressive growth provides a native contrast for optoacoustic imaging. Detection of early stage tumors in the sub-millimeter range, when angiogenesis is not yet established, makes labeling with antibody-gold nanoparticle conjugates necessary. Gold nanoparticles can be tuned to exhibit high absorption in the near infrared, which is the preferred wavelength region for diagnostic purposes due to the low absorption in the surrounding healthy tissue. Moreover, the selectively bound particles can additionally be used for thermal therapy. In the present research project we concentrate on the study of the interaction of laser radiation with gold nanoparticles of different size on a microscopic level. The question addressed is: what is the mechanism for cell damage? One important outcome will be the determination of laser threshold energies necessary to generate a detectable pressure transient and threshold energies that cause cell damage. In parallel we will develop a combined optoacoustic and ultrasound system to comprehensively compare the two imaging modalities. One important part will be the development of a simulation tool for light propagation, pressure wave generation and propagation, pressure detection as well as for image reconstruction. The project not only includes several pioneering works in the fields of optoacoustic imaging and functional/molecular imaging, it is also an excellent example of the impact of the combination of nano- and biotechnology.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Name Institute

Associated projects

Number Title Start Funding scheme
127274 Deep optoacoustic imaging with nanoparticle enhanced contrast 01.10.2009 Project funding (Div. I-III)
127274 Deep optoacoustic imaging with nanoparticle enhanced contrast 01.10.2009 Project funding (Div. I-III)
103872 Contrast enhancement of optoacoustic imaging techniques 01.04.2004 Project funding (Div. I-III)
128408 Optical micromanipulation in an optoacoustic microscope 01.07.2009 International short research visits

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