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Combined force and optical spectroscopy on single bio-molecular machines

English title Combined force and optical spectroscopy on single bio-molecular machines
Applicant Hegner Martin
Number 114145
Funding scheme Project funding (Div. I-III)
Research institution School of Physics Trinity College Dublin
Institution of higher education University of Basel - BS
Main discipline Biophysics
Start/End 01.02.2007 - 31.01.2009
Approved amount 131'300.00
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All Disciplines (3)

Molecular Biology

Keywords (6)

single Molecule Manipulation/Spectroscopy; Cantilever Array Sensing; SPM; Optical tweezers; Nucleic Acids; Protein-Nucleic Acid interaction

Lay Summary (English)

Lay summary
Mitochondrial Import of ProteinsBy a novel combination of single molecule analysis with cell biologi-cal methods we plan to describe the mitochondrial protein transloca-tion reaction at a molecular level. In order to characterize the interaction parameter between preproteins and the components of the import machinery an optical force microscopy assay will be utilized. The affinities of receptor proteins and channel-forming components for single preprotein polypeptides will be determined. A focus will be put on the analysis of the driving forces of mitochondrial preprotein import. We will be able to determine the translocation forces on single preprotein molecules in a quantitative manner. The relative energetic contributions of the electric potential across the inner membrane and the ATP-hydrolysis in the matrix to the translocation process will be defined. The obtained data will clarify the controversial question of the generation of a direct translocation force by the mitochondrial Hsp70 chaperone system.This work is done in collaboration with the Group of PD Dr. Wolfgang Voos University of Freiburg, Germany. The support of the SNF allows hiring one PhD student which is operating the optical tweezers instrument in the group of PD Dr. Martin Hegner at the Institute of Physics, University of Basel, Switzerland. He will develop the single molecule assay and assemble the biomolecular structures to allow nanomechanical experiments.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants


Associated projects

Number Title Start Funding scheme
105953 Single molecule investigations using dynamic force spectroscopy and optical tweezers 01.10.2004 Project funding (Div. I-III)