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Analytical tools for fast phosphoproteome analysis

English title Analytical tools for fast phosphoproteome analysis
Applicant Girault Hubert
Number 113413
Funding scheme Project funding (Div. I-III)
Research institution Laboratoire d'électrochimie physique et analytique EPFL - SB - ISIC - LEPA
Institution of higher education EPF Lausanne - EPFL
Main discipline Physical Chemistry
Start/End 01.01.2007 - 31.12.2009
Approved amount 280'381.00
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Keywords (9)

isoelectric focusing; proteomics; phosphorylation; phosphoproteome; protein phosphorylation; ioelectric fractionation; ampholytes; phosphopeptides; mass spectrometry

Lay Summary (English)

Lead
Lay summary
This proposal is a direct continuation of project 200020-105489/1 that had a two-fold objective: first, to develop proteome fractionation tools based on isoelectric focusing, and second, to develop tagging strategies to increase the information content of mass spectrometric experiment. This proposal follows the same line of research on a more focused target: phosphoproteome analysis. Protein phosphorylation plays a crucial role in many biological processes, such as signal transduction through protein kinase cascades, gene regulation through enzyme activation / inhibition, cytoskeletal activation… Whereas phosphorylation has been the most studied protein post-translational modification so far, all available technologies or methodologies present serious caveats. The gist of this proposal is to develop new tools for proteome fractionation based on isoelectric focusing, and phosphoproteome targeting, isolation and analysis based on metallic ligands.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Associated projects

Number Title Start Funding scheme
127142 Analytical tools for proteome analysis and redoxomics 01.01.2010 Project funding (Div. I-III)
105489 Développement de nouveaux outils analytiques pour la protéomique 01.01.2005 Project funding (Div. I-III)
121570 High throughput SECM imaging: A joint SNF-DFG application 01.01.2009 Project funding (special)

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