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Enzyme Studies Using Molecular and Supramolecular Diversity a) Peptide Dendrimers; b) Selectivity Fingerprints

English title Enzyme Studies Using Molecular and Supramolecular Diversity a) Peptide Dendrimers; b) Selectivity Fingerprints
Applicant Reymond Jean-Louis
Number 107690
Funding scheme Project funding (Div. I-III)
Research institution Departement für Chemie und Biochemie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Organic Chemistry
Start/End 01.04.2005 - 31.03.2007
Approved amount 352'929.00
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Keywords (10)

molecular diversity; enzyme models; dendrimers; combinatorial libraries; fluorescence assays; peptides; enzymes; carbohydrates; combinatorial chemistry; solid-phase synthesis

Lay Summary (English)

Lead
Lay summary
We investigated fundamental aspects of enzyme catalysis in two projects. The first project addressed the development of artificial proteins in the form of tree-like assemblies of amino acids called peptide dendrimers. The tree-like architecture is a topological solution to the protein folding problem. We used combinatorial chemistry to screen large numbers of amino acid sequences for functions, thus mimicking natural selection and evolution processes. We discovered peptide dendrimers with enzyme-like properties, in particular aldolase and esterase dendrimers. The origin of catalysis in these dendrimers was found to reside in the multivalent display of functional groups at the dendrimer surface. We also discovered glycoprotein mimics by multivalent display of carbohydrates on the dendrimer surface. In the second project, we investigated multisubstrate systems to characterize enzyme activities, in particular substrate cocktails and microarrays. These analytical tools allow to functionally characterize and classify enzymes in unprecedented detail.
Direct link to Lay Summary Last update: 21.02.2013

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Associated projects

Number Title Start Funding scheme
100496 Molecular approaches to biocatalyst design and analysis 01.04.2003 Project funding (Div. I-III)
116555 Peptide Dendrimers as Synthetic Artificial Proteins for Catalysis and Binding 01.04.2007 Project funding (Div. I-III)

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